Q8Y0Y6 (AROA_RALSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	RSc0907
ORF Names:	RS04508

Organism

Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

267608 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088281

Sequences

	Sequence		Length	Mass (Da)
	Q8Y0Y6 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 86807CFC7ECE6E2F	FASTA	436	46,145
	10 20 30 40 50 60 MEHLDVGPLK AARGTVKLPG SKSISNRVLL LAALAEGETV VRDLLDSDDT RVMLAALDTL 70 80 90 100 110 120 GVRCEPLGTA NAYRVTGTGG RFPAKSADLF MGNAGTAIRP LTAALALQGG EYTLHGVPRM 130 140 150 160 170 180 HERPIGDLVD GLRQVGARID YTGNEGFPPL AIRAASIRID APIRVRGDVS SQFLTALLMA 190 200 210 220 230 240 LPLVEGSGRP VTIEVVGELI SKPYIEITLN LMARFGVQVE RNGWASFSVP TGVAYRAPGE 250 260 270 280 290 300 IFVEGDASSA SYFLAAGALG GGPVRVEGVG MSSIQGDVRF ADALNRMGAN VMAGDNWIEV 310 320 330 340 350 360 RGVERDDGKL HALELDCNHI PDAAMTLAVA ALFADGTTTL TNIGSWRVKE TDRLTAMATE 370 380 390 400 410 420 LRKLGAAVEE GTDYIRVTPP SHWTAPAGGI DTYDDHRMAM AFSLAAFGPV PVRINDPRCV 430 AKTFPEYFTA FGGIAA

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646052 Genomic DNA. Translation: CAD14609.1.
RefSeq	NP_519028.1. NC_003295.1.
3D structure databases
ProteinModelPortal	Q8Y0Y6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1219715.
GenomeReviews	Gene locus RSc0907 in contig AL646052_GR.
KEGG	rso:RSc0907.
NMPDR	fig|267608.1.peg.907.
PATRIC	20260397. VBIRalSol70888_0929.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646626.
OMA	GADIEWG.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	RSOL267608:RSC0907-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_RALSO

Accession

Primary (citable) accession number: Q8Y0Y6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program