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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei246ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRSOL267608:G1G1H-1249-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:RSc1179
ORF Names:RS04541
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
Proteomesi
  • UP000001436 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001196301 – 465Glutamate--tRNA ligaseAdd BLAST465

Proteomic databases

PRIDEiQ8Y065

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi267608.RSc1179

Structurei

3D structure databases

ProteinModelPortaliQ8Y065
SMRiQ8Y065
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 21"HIGH" regionUniRule annotationAdd BLAST11
Motifi243 – 247"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20 Bacteria
COG0008 LUCA
HOGENOMiHOG000252722
KOiK01885
OMAiAYYAFDT
OrthoDBiPOG091H021W

Family and domain databases

CDDicd00808 GluRS_core, 1 hit
Gene3Di1.10.10.350, 1 hit
1.10.8.70, 1 hit
3.40.50.620, 1 hit
HAMAPiMF_00022 Glu_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR008925 aa-tRNA-synth_I_codon-bd
IPR020752 aa-tRNA-synth_I_codon-bd_sub1
IPR020751 aa-tRNA-synth_I_codon-bd_sub2
IPR001412 aa-tRNA-synth_I_CS
IPR004527 Glu-tRNA-ligase_bac/mito
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR033910 GluRS_core
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF48163 SSF48163, 1 hit
TIGRFAMsiTIGR00464 gltX_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q8Y065-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQRVRTRFA PSPTGFIHLG NIRSALYPWA FARKMKGDFI LRIEDTDVER
60 70 80 90 100
STQEAVDVIL EAMDWLGLDI DEGPFYQMQR MDRYREVIAQ MVAQDLAYPC
110 120 130 140 150
YMSTEELDAL REAQRVRGEK PRYDGTWRPE PGKVLPTPPA GVQPVIRFKN
160 170 180 190 200
PIGGSVVWDD AVKGRIEISN DELDDLVIAR PDGTPTYNFC VVVDDMDMRI
210 220 230 240 250
THVIRGDDHV NNTPRQINIL RALGGTPPIY AHLPTVLNEQ GEKMSKRHGA
260 270 280 290 300
MSVTGYRDAG YLPEAVVNYL ARLGWAHGDA EIFSREQFVE WFDLEHLGKS
310 320 330 340 350
PAQYNPEKLA WLNNHYIKQA DNARLAELVK PFIEALGGRV EGGPQLADVM
360 370 380 390 400
ALVKDRANTL QEVAQTALLF YRTEISVAPE LAAQHLTDEV RPGIMALADK
410 420 430 440 450
LGTLPEWKRE AIGAAFKEVL GAHGWKMPKL AMPVRLLVAG QLQTPSIDAV
460
LELFGREVVL RRLAG
Length:465
Mass (Da):52,199
Last modified:April 30, 2003 - v2
Checksum:i772FB42CAE37EB9C
GO

Sequence cautioni

The sequence CAD14881 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA Translation: CAD14881.1 Different initiation.
RefSeqiWP_043876578.1, NC_003295.1

Genome annotation databases

EnsemblBacteriaiCAD14881; CAD14881; RSc1179
GeneIDi1219995
KEGGirso:RSc1179
PATRICifig|267608.8.peg.1198

Similar proteinsi

Entry informationi

Entry nameiSYE_RALSO
AccessioniPrimary (citable) accession number: Q8Y065
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: May 23, 2018
This is version 90 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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