Reviewed, UniProtKB/Swiss-Prot Q8XZJ6 (DEF2_RALSO)

Names and origin

Protein names

Recommended name:
    Peptide deformylase 2
      Short name=PDF 2
    EC=3.5.1.88
Alternative name(s):
    Polypeptide deformylase 2

Gene names

Name:	def2
Ordered Locus Names:	RSc1399
ORF Names:	RS05293

Organism

Ralstonia solanacearum (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP MF_00163
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity. HAMAP MF_00163
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Peptide deformylase 2 HAMAP MF_00163		PRO_0000082825
Sites
	Active site	142	1	By similarity
	Metal binding	99	1	Iron By similarity
	Metal binding	141	1	Iron By similarity
	Metal binding	145	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XZJ6-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: BC1E8FE92EDC1037	FASTA	177	19,950
	10 20 30 40 50 60 MIRPILKMGD SRLLRVAKPV QRFQTPELTA LIEDMFDTMD AARGAGLAAP QIGVDLQVVI 70 80 90 100 110 120 FGFDRNDRYP DAPAVPKTVL INPTIEPLSD AMEDGWEGCL SVPGLRGVVP RYTRLRYTGY 130 140 150 160 170 DQHGHAIDRI AEGFHARVVQ HECDHLQGIL YPMRVQDFTR FGFTEILFPE LPAHHND

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646052 Genomic DNA. Translation: CAD15101.1.
RefSeq	NP_519520.1.
3D structure databases
SMR	Q8XZJ6. Positions 2-167.
ModBase	Search...
Genome annotation databases
GeneID	1220223.
GenomeReviews	Gene locus RSc1399 in contig AL646052_GR.
KEGG	rso:RSc1399.
NMPDR	fig|267608.1.peg.1399.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG665227.
OMA	HNERYPD.
Enzyme and pathway databases
BioCyc	RSOL267608:RSC1399-MONOMER.
BRENDA	3.5.1.88. 97066.
Family and domain databases
HAMAP	MF_00163. Pep_deformylase. [Tree]
InterPro	IPR000181. Fmet_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
TIGRFAMs	TIGR00079. pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF2_RALSO

Accession

Primary (citable) accession number: Q8XZJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2002
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)