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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-1424-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:RSc1402
ORF Names:RS05290
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000001436 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 861861Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192757Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi267608.RSc1402.

Structurei

3D structure databases

ProteinModelPortaliQ8XZJ3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini442 – 558117HDUniRule annotationAdd
BLAST
Domaini680 – 76384ACT 1UniRule annotationAdd
BLAST
Domaini792 – 86170ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 322322UridylyltransferaseAdd
BLAST
Regioni323 – 679357Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHTLFWIA.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
IPR010043. UTase/UR.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8XZJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHTAAAATPA TSPRDILKAE RAHLFAQFEQ HANVNLLVTK LARAVDQALI
60 70 80 90 100
LLWQDEGMPD TCALVAVGGY GRGELFPHSD VDILLLLPQT ADKALETRLE
110 120 130 140 150
AFIGHCWDMG LDIGSSVRTV DECISEATQD VTVCTSLLEA RLLTGDEGLY
160 170 180 190 200
RTFETHYQGH LDAADFYQSK MLEMRQRHAK YQDTPYSLEP NCKESPGGLR
210 220 230 240 250
DLQVILWMTR AAGFGSSWNE LLVNQLLTRR EAKELAANER LLKTIRARLH
260 270 280 290 300
LLAGRRQDVL VFDLQTQLGE AFGYRPNAAK RTSEQLMRRY YWAAKAVTQL
310 320 330 340 350
NTVVLQNIEA RLFPTELGIT RTINGRFVER QGMLEIADPE LYQREPAAIL
360 370 380 390 400
ETFLVYEQTR GVKGLAANTL RALYNARTQM DARWRRDPAN RATFLSILQQ
410 420 430 440 450
PQGITHALRL MNQTSVLGRY LVNFRRIVGQ MQHDLFHVYT VDQHILMVVR
460 470 480 490 500
NVRRFAIVEH AHEFPFCSQL MANFDKPWVL TVAALFHDIA KGRGGDHSVL
510 520 530 540 550
GMADARRFCK QHGIASEDAD LIVWLVEHHL TMSQVAQKQD LGDPEVIRHF
560 570 580 590 600
ADQVGSERYL SALYLLTVAD IRGTSPKVWN AWKAKLLEDL YRITLRVLGG
610 620 630 640 650
ATTDPHAVLE GRKEEARVLL RLAAMDPHAH EALWAQLDVG VFLRHDARDI
660 670 680 690 700
AWFTRHFYNR VDTTLPIVRA RISPVGEGLQ VAVYSPDRPD LFARICGYFE
710 720 730 740 750
RKGLTILDAK IHTTKHGYAL DTFQVADPGS GLVEPGHYRD IITLVEHELA
760 770 780 790 800
ELIARETVLA EPPRGRISRQ SRSFPIKPRV DLRPDERGQY YLLSLSATDR
810 820 830 840 850
TGLLYAIARV LARHRVSVHT ARINTLGERV EDVFLLDGRR LTQDNKLQLA
860
LESELLEALA I
Length:861
Mass (Da):97,493
Last modified:March 1, 2002 - v1
Checksum:i3C4F9172C180F7C0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD15104.1.
RefSeqiWP_011001351.1. NC_003295.1.

Genome annotation databases

EnsemblBacteriaiCAD15104; CAD15104; RSc1402.
GeneIDi1220226.
KEGGirso:RSc1402.
PATRICi20261435. VBIRalSol70888_1432.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD15104.1.
RefSeqiWP_011001351.1. NC_003295.1.

3D structure databases

ProteinModelPortaliQ8XZJ3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267608.RSc1402.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD15104; CAD15104; RSc1402.
GeneIDi1220226.
KEGGirso:RSc1402.
PATRICi20261435. VBIRalSol70888_1432.

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHTLFWIA.
OrthoDBiEOG6CCH44.

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-1424-MONOMER.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
IPR010043. UTase/UR.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GMI1000.

Entry informationi

Entry nameiGLND_RALSO
AccessioniPrimary (citable) accession number: Q8XZJ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: March 1, 2002
Last modified: July 22, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.