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Q8XZJ3 (GLND_RALSO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme

Short name=UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein

Including the following 2 domains:

  1. [Protein-PII] uridylyltransferase
    Short name=PII uridylyltransferase
    Short name=UTase
    EC=2.7.7.59
  2. [Protein-PII]-UMP uridylyl-removing enzyme
    Short name=UR
    EC=3.1.4.-
Gene names
Name:glnD
Ordered Locus Names:RSc1402
ORF Names:RS05290
OrganismRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]
Taxonomic identifier267608 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia

Protein attributes

Sequence length861 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity. HAMAP-Rule MF_00277

Catalytic activity

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP-Rule MF_00277

Uridylyl-[protein-PII] + H2O = UMP + [protein-PII]. HAMAP-Rule MF_00277

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00277

Enzyme regulation

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity. HAMAP-Rule MF_00277

Domain

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity. HAMAP-Rule MF_00277

Sequence similarities

Belongs to the GlnD family.

Contains 2 ACT domains.

Contains 1 HD domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 861861Bifunctional uridylyltransferase/uridylyl-removing enzyme HAMAP-Rule MF_00277
PRO_0000192757

Regions

Domain442 – 558117HD
Domain680 – 76384ACT 1
Domain792 – 86170ACT 2
Region1 – 322322Uridylyltransferase HAMAP-Rule MF_00277
Region323 – 679357Uridylyl-removing HAMAP-Rule MF_00277

Sequences

Sequence LengthMass (Da)Tools
Q8XZJ3 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 3C4F9172C180F7C0

FASTA86197,493
        10         20         30         40         50         60 
MHTAAAATPA TSPRDILKAE RAHLFAQFEQ HANVNLLVTK LARAVDQALI LLWQDEGMPD 

        70         80         90        100        110        120 
TCALVAVGGY GRGELFPHSD VDILLLLPQT ADKALETRLE AFIGHCWDMG LDIGSSVRTV 

       130        140        150        160        170        180 
DECISEATQD VTVCTSLLEA RLLTGDEGLY RTFETHYQGH LDAADFYQSK MLEMRQRHAK 

       190        200        210        220        230        240 
YQDTPYSLEP NCKESPGGLR DLQVILWMTR AAGFGSSWNE LLVNQLLTRR EAKELAANER 

       250        260        270        280        290        300 
LLKTIRARLH LLAGRRQDVL VFDLQTQLGE AFGYRPNAAK RTSEQLMRRY YWAAKAVTQL 

       310        320        330        340        350        360 
NTVVLQNIEA RLFPTELGIT RTINGRFVER QGMLEIADPE LYQREPAAIL ETFLVYEQTR 

       370        380        390        400        410        420 
GVKGLAANTL RALYNARTQM DARWRRDPAN RATFLSILQQ PQGITHALRL MNQTSVLGRY 

       430        440        450        460        470        480 
LVNFRRIVGQ MQHDLFHVYT VDQHILMVVR NVRRFAIVEH AHEFPFCSQL MANFDKPWVL 

       490        500        510        520        530        540 
TVAALFHDIA KGRGGDHSVL GMADARRFCK QHGIASEDAD LIVWLVEHHL TMSQVAQKQD 

       550        560        570        580        590        600 
LGDPEVIRHF ADQVGSERYL SALYLLTVAD IRGTSPKVWN AWKAKLLEDL YRITLRVLGG 

       610        620        630        640        650        660 
ATTDPHAVLE GRKEEARVLL RLAAMDPHAH EALWAQLDVG VFLRHDARDI AWFTRHFYNR 

       670        680        690        700        710        720 
VDTTLPIVRA RISPVGEGLQ VAVYSPDRPD LFARICGYFE RKGLTILDAK IHTTKHGYAL 

       730        740        750        760        770        780 
DTFQVADPGS GLVEPGHYRD IITLVEHELA ELIARETVLA EPPRGRISRQ SRSFPIKPRV 

       790        800        810        820        830        840 
DLRPDERGQY YLLSLSATDR TGLLYAIARV LARHRVSVHT ARINTLGERV EDVFLLDGRR 

       850        860 
LTQDNKLQLA LESELLEALA I 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL646052 Genomic DNA. Translation: CAD15104.1.
RefSeqNP_519523.1. NC_003295.1.

3D structure databases

ProteinModelPortalQ8XZJ3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING267608.RSc1402.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD15104; CAD15104; RSc1402.
GeneID1220226.
KEGGrso:RSc1402.
PATRIC20261435. VBIRalSol70888_1432.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2844.
HOGENOMHOG000261778.
KOK00990.
OMAHHLLMSV.
OrthoDBEOG6CCH44.

Enzyme and pathway databases

BioCycRSOL267608:GCVU-1424-MONOMER.

Family and domain databases

Gene3D1.10.3210.10. 1 hit.
HAMAPMF_00277. PII_uridylyl_transf.
InterProIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFPIRSF006288. PII_uridyltransf. 1 hit.
SMARTSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsTIGR01693. UTase_glnD. 1 hit.
PROSITEPS51671. ACT. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLND_RALSO
AccessionPrimary (citable) accession number: Q8XZJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: March 1, 2002
Last modified: June 11, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families