Q8XZH8 (LPXB_RALSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipid-A-disaccharide synthase
EC=2.4.1.182

Gene names

Name:	lpxB
Ordered Locus Names:	RSc1417
ORF Names:	RS05275

Organism

Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

267608 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia › Ralstonia solanacearum

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP-Rule MF_00392
Catalytic activity	UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate = UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. HAMAP-Rule MF_00392
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 5/6. HAMAP-Rule MF_00392
Sequence similarities	Belongs to the LpxB family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid biosynthesis Lipid metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	lipid-A-disaccharide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Lipid-A-disaccharide synthase HAMAP-Rule MF_00392		PRO_0000190180

Sequences

	Sequence		Length	Mass (Da)
	Q8XZH8 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 8AC1151064879FA9	FASTA	390	42,549
	10 20 30 40 50 60 MTQPPAAPAR RIGMAAGEAS GDLLASLLLK GLHARLPRDV AYEGIGGARM AEQGFASHWP 70 80 90 100 110 120 MHKLSVNGYV EVLGQLREIL AIRRELKQNL LADPPMAFIG VDAPDFNFNV EIAMRRAGVP 130 140 150 160 170 180 VVHFVSPSIW AWRAGRIKTI ARAVDHILCL FPFEPEIYAR AGIPATYVGH PLADEIPLEP 190 200 210 220 230 240 DVEGARSRLG LPLGRKVVAV LPGSRNSEVK LLGPTLFAAM ARMQAVEPDL AFVLPAANAM 250 260 270 280 290 300 LRERIDAMRA EHPGLHLWVV DGQSHAAMEA ADVILLASGT ATLEAALYKK PMVITYKVPW 310 320 330 340 350 360 LTAQIMKRKG YLPYVGLPNI LSGRFVVPEL LQDDATPEAL ARETLLQLSD HANAAFLREH 370 380 390 FTQMHLTLKQ NMADIGAQVV VDLLRGRGKL

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646052 Genomic DNA. Translation: CAD15119.1.
RefSeq	NP_519538.1. NC_003295.1.
3D structure databases
ProteinModelPortal	Q8XZH8.
ModBase	Search...
Protein-protein interaction databases
STRING	267608.RSc1417.
Protein family/group databases
CAZy	GT19. Glycosyltransferase Family 19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD15119; CAD15119; RSc1417.
GeneID	1220241.
KEGG	rso:RSc1417.
PATRIC	20261469. VBIRalSol70888_1449.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0763.
HOGENOM	HOG000018003.
KO	K00748.
OMA	PTVWAWR.
ProtClustDB	PRK00025.
Enzyme and pathway databases
UniPathway	UPA00359; UER00481.
Family and domain databases
HAMAP	MF_00392. LpxB.
InterPro	IPR003835. Glyco_trans_19. [Graphical view]
Pfam	PF02684. LpxB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00215. lpxB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXB_RALSO

Accession

Primary (citable) accession number: Q8XZH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program