Q8XZC3 (BIOF_RALSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
8-amino-7-oxononanoate synthase
Short name=AONS
EC=2.3.1.47

Alternative name(s):
7-keto-8-amino-pelargonic acid synthase
Short name=7-KAP synthase
Short name=KAPA synthase
8-amino-7-ketopelargonate synthase

Gene names

Name:	bioF
Ordered Locus Names:	RSc1478

Organism

Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

267608 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia › Ralstonia solanacearum

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide By similarity. HAMAP-Rule MF_01693
Catalytic activity	Pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein]. HAMAP-Rule MF_01693
Cofactor	Pyridoxal phosphate By similarity. HAMAP-Rule MF_01693
Pathway	Cofactor biosynthesis; biotin biosynthesis. HAMAP-Rule MF_01693
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01693
Sequence similarities	Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	8-amino-7-oxononanoate synthase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	8-amino-7-oxononanoate synthase HAMAP-Rule MF_01693		PRO_0000381090
Regions
	Region	117 – 118	2	Pyridoxal phosphate binding By similarity
	Region	220 – 223	4	Pyridoxal phosphate binding By similarity
	Region	251 – 254	4	Pyridoxal phosphate binding By similarity
Sites
	Binding site	21	1	Substrate By similarity
	Binding site	149	1	Substrate By similarity
	Binding site	195	1	Pyridoxal phosphate By similarity
	Binding site	374	1	Substrate By similarity
Amino acid modifications
	Modified residue	254	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XZC3 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 1CED02079365489F	FASTA	416	43,677
	10 20 30 40 50 60 MRLLDDLRAG LDAIDAAHLR RVRRTAYSPT DRSQRIDVPG GEPREILGFC GNDYLGLAAH 70 80 90 100 110 120 PALMQAVGEG ARRYGFGSGA SHLVSGHSVA HARLEARMAA LQAPHIPDAD ALCFCTGYMA 130 140 150 160 170 180 NMAVVSAMAQ AGGIQPAQDC TVFSDALNHA SLIDGARLSR AAVKVYPHVD LAALEAQLAA 190 200 210 220 230 240 CGSRNKLIVT DGVFSMDGDI APLPELLALA ERFDAWLIVD DAHGLGVLGA DGVGVLSHFG 250 260 270 280 290 300 LRSERLIYIG TFGKAAGGAG ACVVAHRMAV DWLVQRARTY IFTTATPPAV ACGVEAALDL 310 320 330 340 350 360 IAGEEGAARR ARLAGHIARW SDHAQRLAAR FGWQWMPSPT AIQPLVIGEN APALALAAAL 370 380 390 400 410 EREGIRVAAI RPPTVPAGTA RLRITLSAAH TEDDIERLAR ALEAAGQSQQ SAARAA

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646052 Genomic DNA. Translation: CAD15180.1.
RefSeq	NP_519599.1. NC_003295.1.
3D structure databases
ProteinModelPortal	Q8XZC3.
ModBase	Search...
Protein-protein interaction databases
STRING	267608.RSc1478.
Proteomic databases
PRIDE	Q8XZC3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD15180; CAD15180; RSc1478.
GeneID	1220307.
KEGG	rso:RSc1478.
PATRIC	20261607. VBIRalSol70888_1513.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0156.
HOGENOM	HOG000221021.
KO	K00652.
OMA	DFYAITQ.
ProtClustDB	PRK05958.
Enzyme and pathway databases
BioCyc	RSOL267608:GCVU-1505-MONOMER.
UniPathway	UPA00078.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
HAMAP	MF_01693. BioF_aminotrans_2.
InterPro	IPR022834. Amino_oxononanoate_synth. IPR004839. Aminotransferase_I/II. IPR004723. BioF. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00858. bioF. 1 hit.
PROSITE	PS00599. AA_TRANSFER_CLASS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BIOF_RALSO

Accession

Primary (citable) accession number: Q8XZC3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 1, 2002
Last modified:	May 29, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program