Q8XYW3 (ISPD_RALSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
EC=2.7.7.60

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
MEP cytidylyltransferase
Short name=MCT

Gene names

Name:	ispD
Ordered Locus Names:	RSc1643
ORF Names:	RS04018

Organism

Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

267608 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108
Sequence similarities	Belongs to the IspD family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108		PRO_0000075607
Sites
	Site	23	1	Transition state stabilizer By similarity
	Site	30	1	Transition state stabilizer By similarity
	Site	171	1	Positions MEP for the nucleophilic attack By similarity
	Site	227	1	Positions MEP for the nucleophilic attack By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XYW3 [UniParc]. Last modified March 5, 2002. Version 1. Checksum: 22CC2D6CFAE3B46E	FASTA	253	26,134
	10 20 30 40 50 60 MSVSSRPGRR RFALIPSAGT GTRAGGDLPK QYQPLAGRPM LWHTVQAFLA SAAIDRVVVV 70 80 90 100 110 120 TSPGAPALAA RFPGLGFDAA RLVEVDCGGD SRHASVTNGL AALRGLGAHE DDWVLVHDAA 130 140 150 160 170 180 RPGLTPALIA RLVGAVEADG GQAVGGILAL PVADTLKRAD AGQHIAETVP RGGLWQAQTP 190 200 210 220 230 240 QMFPLGLLQR ALSEALTAQR IVTDEASAIE AAGHRPLLVA GALRNFKVTY PDDFALAEAI 250 LAGAAPTQED SQA

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646052 Genomic DNA. Translation: CAD15345.1.
RefSeq	NP_519764.1. NC_003295.1.
3D structure databases
ProteinModelPortal	Q8XYW3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1220478.
GenomeReviews	Gene locus RSc1643 in contig AL646052_GR.
KEGG	rso:RSc1643.
NMPDR	fig|267608.1.peg.1643.
PATRIC	20261961. VBIRalSol70888_1684.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672839.
OMA	FPQGAIL.
ProtClustDB	PRK00155.
Enzyme and pathway databases
BioCyc	RSOL267608:RSC1643-MONOMER.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ISPD_RALSO

Accession

Primary (citable) accession number: Q8XYW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 5, 2002
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program