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Protein

tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC

Gene

mnmC

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the last two steps in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm5s2U34 to nm5s2U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm5s2U34, to form mnm5s2U34.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + tRNA containing 5-aminomethyl-2-thiouridine = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate.UniRule annotation

Cofactori

FADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-1742-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmCUniRule annotation
Short name:
tRNA mnm(5)s(2)U biosynthesis bifunctional proteinUniRule annotation
Including the following 2 domains:
tRNA (mnm(5)s(2)U34)-methyltransferaseUniRule annotation (EC:2.1.1.61UniRule annotation)
FAD-dependent cmnm(5)s(2)U34 oxidoreductaseUniRule annotation (EC:1.5.-.-UniRule annotation)
Gene namesi
Name:mnmCUniRule annotation
Ordered Locus Names:RSc1709
ORF Names:RS02895
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
Proteomesi
  • UP000001436 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 657657tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmCPRO_0000095024Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi267608.RSc1709.

Structurei

3D structure databases

ProteinModelPortaliQ8XYP9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 233233tRNA (mnm(5)s(2)U34)-methyltransferaseAdd
BLAST
Regioni256 – 657402FAD-dependent cmnm(5)s(2)U34 oxidoreductaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family.UniRule annotation
In the C-terminal section; belongs to the DAO family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CWG. Bacteria.
COG0665. LUCA.
COG4121. LUCA.
HOGENOMiHOG000218143.
KOiK15461.
OMAiKSVLCYD.
OrthoDBiEOG61P6QH.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
3.50.50.60. 3 hits.
HAMAPiMF_01102. MnmC.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR008471. MnmC-like_methylTransf.
IPR029063. SAM-dependent_MTases.
IPR023032. tRNA_MAMT_biosynth_bifunc_MnmC.
IPR017610. tRNA_S-uridine_synth_MnmC_C.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF05430. Methyltransf_30. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR03197. MnmC_Cterm. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8XYP9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRGLILATP QLPPDGTPFS ARYDDVYHST EGGLAQAHHV FLGGNGLPGG
60 70 80 90 100
WQGKRSFTIV ETGFGQGLNF LATWAAWRDD PQRCGRLDFV SVEKHPFDRA
110 120 130 140 150
GLALVHAQEG ALASLAEALR QAWPVPVPGV HRLVFDAGRV TLTLMLGDAE
160 170 180 190 200
TLLPQLSCAA DAFYLDGFSP AKNPDLWQPG VFKQLARIAR GGATLATYTA
210 220 230 240 250
AGAVRHGLRE AGFEVRKAPG FGRKRDMTVA AFPTHWRTRR GPAEAPVWPE
260 270 280 290 300
RHAIVLGAGL AGCSVAERLA ARGWRITLID EHEAPAGGAS AHRAAAMHPH
310 320 330 340 350
VSIDDSVLSR LSRAGNLLAR RHWEALDRAG FATGFHPTGV LQLAEHADDA
360 370 380 390 400
AEQQRIVQAL GFPADFIAWL DAARAADRVG AGVPQGGWWF AQAGWVAPPD
410 420 430 440 450
ICRAALAAAG EAVELRWRTR IESLLRHDAG WRACDAQGNV IAHAPVVVLA
460 470 480 490 500
NSLDAVRLAP LASAALKPVR GQLTDVPVAA LEDGVPWPRA VVCGDGYLLP
510 520 530 540 550
RERGAPTVRV GSSFQPGETD PDARTADHAA NLRRLAGLQP AHAAALARVD
560 570 580 590 600
PARLLGYVGV RCVSANRLPL IGAVADEAAI MAPGFRLRGP HAALPRVPGL
610 620 630 640 650
YAALAYGSRG LTWSVLGAEL LAAQIDGGPL PLESELAAAL DPGRFLMRAL

RHGKHAA
Length:657
Mass (Da):69,668
Last modified:March 1, 2002 - v1
Checksum:iD55F5032A07F52E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD15411.1.
RefSeqiWP_011001650.1. NC_003295.1.

Genome annotation databases

EnsemblBacteriaiCAD15411; CAD15411; RSc1709.
GeneIDi1220545.
KEGGirso:RSc1709.
PATRICi20262099. VBIRalSol70888_1752.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD15411.1.
RefSeqiWP_011001650.1. NC_003295.1.

3D structure databases

ProteinModelPortaliQ8XYP9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267608.RSc1709.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD15411; CAD15411; RSc1709.
GeneIDi1220545.
KEGGirso:RSc1709.
PATRICi20262099. VBIRalSol70888_1752.

Phylogenomic databases

eggNOGiENOG4105CWG. Bacteria.
COG0665. LUCA.
COG4121. LUCA.
HOGENOMiHOG000218143.
KOiK15461.
OMAiKSVLCYD.
OrthoDBiEOG61P6QH.

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-1742-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
3.50.50.60. 3 hits.
HAMAPiMF_01102. MnmC.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR008471. MnmC-like_methylTransf.
IPR029063. SAM-dependent_MTases.
IPR023032. tRNA_MAMT_biosynth_bifunc_MnmC.
IPR017610. tRNA_S-uridine_synth_MnmC_C.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF05430. Methyltransf_30. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR03197. MnmC_Cterm. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GMI1000.

Entry informationi

Entry nameiMNMC_RALSO
AccessioniPrimary (citable) accession number: Q8XYP9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: March 1, 2002
Last modified: November 11, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.