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Reviewed, UniProtKB/Swiss-Prot Q8XYN6 (PUR4_RALSO)

Last modified February 9, 2010. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase
Gene names
Name: purL
Ordered Locus Names: RSc1722
ORF Names: RS02912
OrganismRalstonia solanacearum (Pseudomonas solanacearum) [Complete proteome] [HAMAP]
Taxonomic identifier305 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

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Protein attributes

Sequence length1369 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419

Subunit structure

Monomer. HAMAP MF_00419

Subcellular location

Cytoplasm HAMAP MF_00419.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

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Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13691369Phosphoribosylformylglycinamidine synthase HAMAP MF_00419
PRO_0000100416

Regions

Domain1116 – 1369254Glutamine amidotransferase type-1
Nucleotide binding330 – 34112ATP Potential

Sites

Active site12091Nucleophile By similarity
Active site13301 By similarity
Active site13321 By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q8XYN6-1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: FAB0B960D77C6A1F

FASTA1,369146,481
        10         20         30         40         50         60 
MAHFSCFPGA LALSAFRQQR LLSTLKRIDP EIDAVSAQYL HFVAADAPLS ADDAARVQAL 

        70         80         90        100        110        120 
LTYGSPASAE TEGDRFVVIP RFGTISPWAS KATEIARHCA LPQIHRIERG VEFTVTCKKG 

       130        140        150        160        170        180 
LLRGLTGGRK QLDEATRAAV AAHLHDRMTE IVVATREAGY GLFDVLPAKA LRFVDLGSGD 

       190        200        210        220        230        240 
AAAGRGALEA ANTEMGLALS DDEIDYLVDA YRKLGRNPTD VELMMFAQAN SEHCRHKIFN 

       250        260        270        280        290        300 
ADWTIDGETQ DKSLFAMIRN THQLAPQGTV VAYSDNAAVM EGGMAERWFP HAGTDGETGV 

       310        320        330        340        350        360 
PQYGRREALT HTLMKVETHN HPTAISPFPG ASTGAGGEIR DEGATGRGAK PKAGLTGFTV 

       370        380        390        400        410        420 
SNLLLPEAVQ SWENARDTAQ PAAQRNPGDT APGPVGKPDR IASPLQIMIE GPIGGAAFNN 

       430        440        450        460        470        480 
EFGRPNLGGY FRVYEQNVGG TVRGYHKPIM IAGGIGNIDA GHTHKHGLPA GTLLVQLGGP 

       490        500        510        520        530        540 
GMRIGMGGGA ASSMATGTNT ADLDFDSVQR GNPEMQRRAQ EVINACWALG EDNPILSIHD 

       550        560        570        580        590        600 
VGAGGISNAF PELVDGADRG ARFDLRQVHL EESGLSPAEI WCNESQERYT LAIAPGDFPR 

       610        620        630        640        650        660 
FQAMCERERA PFSVVGFATE EQQLQVVDGD APADAVEHFP VNMPMDVLLG KPPRMHRDVR 

       670        680        690        700        710        720 
RVAQALPEVD VTGLDLETVA RDVLRHPTVA SKSFLITIGD RTVGGLNTRD QMVGPWQVPV 

       730        740        750        760        770        780 
ADVAVTTLDY KGYAGEAMTM GERTPLAVID APASGRMAIG EAITNIAAAP IASLAQLKLS 

       790        800        810        820        830        840 
ANWMAACGVD GEDARLYDTV KAVGMELCPA LGISIPVGKD SLSMRTKWDD AGEAKEVVAP 

       850        860        870        880        890        900 
VSLIVSAFAP VTDVRKTLTP QLKPVASAGE AADTTLIVID LGHGKHRLGG SILAQVTQQI 

       910        920        930        940        950        960 
GNSVPDVGDA EDLKRFFAAI QQLNAAGMLL AYHDRSDGGL WATVCEMAFA GHCGVSINVD 

       970        980        990       1000       1010       1020 
MLTLDGAHAS DYGDAKNWAQ QVSGRRADMT LRALFAEELG AVIQVPAAQR DAVFAVLREH 

      1030       1040       1050       1060       1070       1080 
GLAACSHVIG APNASGQIEI WRDAKKVFSA PRIELQRAWT DVSWRIASLR DNPECTQSEY 

      1090       1100       1110       1120       1130       1140 
DRLLDAEDPG ISPNLTFDLA EDVAAPFIAT GARPRMAILR EQGVNSQVEM AYAMDKAGFD 

      1150       1160       1170       1180       1190       1200 
AYDVHMSDLL AGRTRLADFK GFVACGGFSY GDVLGAGEGW AKTILFNGML AEQFAAFFNR 

      1210       1220       1230       1240       1250       1260 
ADSIALGVCN GCQMMANLAP IIPGAGAWPK FTRNQSEQYE GRLVTVQVEA SPSIFYAGME 

      1270       1280       1290       1300       1310       1320 
GSRIPIVVAH GEGYADFSQQ GDIGKVAVGL RYVDNRGEVT QTYPLNPNGS PQGIASVTTH 

      1330       1340       1350       1360 
DGRFTVLMPH PERVFRAVQM SWHPKDWAAA GDGSSPWMRM FRNARKQMG

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL646052 Genomic DNA. Translation: CAD15424.1.
RefSeqNP_519843.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID1220560.
GenomeReviewsGene locus RSc1722 in contig AL646052_GR.
KEGGrso:RSc1722.
NMPDRfig|267608.1.peg.1722.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG335309.
OMAERGIAYY.

Enzyme and pathway databases

BioCycRSOL267608:RSC1722-MONOMER.
BRENDA6.3.5.3. 97066.

Family and domain databases

HAMAPMF_00419. PurL_1.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR016188. PurM_N-like.
[Graphical view]
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePUR4_RALSO
AccessionPrimary (citable) accession number: Q8XYN6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: March 1, 2002
Last modified: February 9, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents