Reviewed, UniProtKB/Swiss-Prot Q8XYN6 (PUR4_RALSO)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	RSc1722
ORF Names:	RS02912

Organism

Ralstonia solanacearum (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	1369 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer. HAMAP MF_00419
Subcellular location	Cytoplasm. HAMAP MF_00419
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1369	1369	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000100416
Regions
	Domain	1116 – 1369	254	Glutamine amidotransferase type-1
	Nucleotide binding	330 – 341	12	ATP Potential
Sites
	Active site	1209	1	Nucleophile By similarity
	Active site	1330	1	By similarity
	Active site	1332	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XYN6-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: FAB0B960D77C6A1F	FASTA	1,369	146,481
	10 20 30 40 50 60 MAHFSCFPGA LALSAFRQQR LLSTLKRIDP EIDAVSAQYL HFVAADAPLS ADDAARVQAL 70 80 90 100 110 120 LTYGSPASAE TEGDRFVVIP RFGTISPWAS KATEIARHCA LPQIHRIERG VEFTVTCKKG 130 140 150 160 170 180 LLRGLTGGRK QLDEATRAAV AAHLHDRMTE IVVATREAGY GLFDVLPAKA LRFVDLGSGD 190 200 210 220 230 240 AAAGRGALEA ANTEMGLALS DDEIDYLVDA YRKLGRNPTD VELMMFAQAN SEHCRHKIFN 250 260 270 280 290 300 ADWTIDGETQ DKSLFAMIRN THQLAPQGTV VAYSDNAAVM EGGMAERWFP HAGTDGETGV 310 320 330 340 350 360 PQYGRREALT HTLMKVETHN HPTAISPFPG ASTGAGGEIR DEGATGRGAK PKAGLTGFTV 370 380 390 400 410 420 SNLLLPEAVQ SWENARDTAQ PAAQRNPGDT APGPVGKPDR IASPLQIMIE GPIGGAAFNN 430 440 450 460 470 480 EFGRPNLGGY FRVYEQNVGG TVRGYHKPIM IAGGIGNIDA GHTHKHGLPA GTLLVQLGGP 490 500 510 520 530 540 GMRIGMGGGA ASSMATGTNT ADLDFDSVQR GNPEMQRRAQ EVINACWALG EDNPILSIHD 550 560 570 580 590 600 VGAGGISNAF PELVDGADRG ARFDLRQVHL EESGLSPAEI WCNESQERYT LAIAPGDFPR 610 620 630 640 650 660 FQAMCERERA PFSVVGFATE EQQLQVVDGD APADAVEHFP VNMPMDVLLG KPPRMHRDVR 670 680 690 700 710 720 RVAQALPEVD VTGLDLETVA RDVLRHPTVA SKSFLITIGD RTVGGLNTRD QMVGPWQVPV 730 740 750 760 770 780 ADVAVTTLDY KGYAGEAMTM GERTPLAVID APASGRMAIG EAITNIAAAP IASLAQLKLS 790 800 810 820 830 840 ANWMAACGVD GEDARLYDTV KAVGMELCPA LGISIPVGKD SLSMRTKWDD AGEAKEVVAP 850 860 870 880 890 900 VSLIVSAFAP VTDVRKTLTP QLKPVASAGE AADTTLIVID LGHGKHRLGG SILAQVTQQI 910 920 930 940 950 960 GNSVPDVGDA EDLKRFFAAI QQLNAAGMLL AYHDRSDGGL WATVCEMAFA GHCGVSINVD 970 980 990 1000 1010 1020 MLTLDGAHAS DYGDAKNWAQ QVSGRRADMT LRALFAEELG AVIQVPAAQR DAVFAVLREH 1030 1040 1050 1060 1070 1080 GLAACSHVIG APNASGQIEI WRDAKKVFSA PRIELQRAWT DVSWRIASLR DNPECTQSEY 1090 1100 1110 1120 1130 1140 DRLLDAEDPG ISPNLTFDLA EDVAAPFIAT GARPRMAILR EQGVNSQVEM AYAMDKAGFD 1150 1160 1170 1180 1190 1200 AYDVHMSDLL AGRTRLADFK GFVACGGFSY GDVLGAGEGW AKTILFNGML AEQFAAFFNR 1210 1220 1230 1240 1250 1260 ADSIALGVCN GCQMMANLAP IIPGAGAWPK FTRNQSEQYE GRLVTVQVEA SPSIFYAGME 1270 1280 1290 1300 1310 1320 GSRIPIVVAH GEGYADFSQQ GDIGKVAVGL RYVDNRGEVT QTYPLNPNGS PQGIASVTTH 1330 1340 1350 1360 DGRFTVLMPH PERVFRAVQM SWHPKDWAAA GDGSSPWMRM FRNARKQMG

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL646052 Genomic DNA. Translation: CAD15424.1.
RefSeq	NP_519843.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1220560.
GenomeReviews	Gene locus RSc1722 in contig AL646052_GR.
KEGG	rso:RSc1722.
NMPDR	fig|267608.1.peg.1722.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8XYN6.
OMA	ERGIAYY.
Enzyme and pathway databases
BioCyc	RSOL267608:RSC1722-MON.
BRENDA	6.3.5.3. 97066.
Family and domain databases
HAMAP	MF_00419. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_RALSO

Accession

Primary (citable) accession number: Q8XYN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)