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Protein

Urease subunit alpha

Gene

ureC

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit alpha (ureC), Urease subunit beta (ureB), Urease subunit gamma (ureA)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi135 – 1351Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi137 – 1371Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi218 – 2181Nickel 1; via carbamate groupUniRule annotation
Metal bindingi218 – 2181Nickel 2; via carbamate groupUniRule annotation
Binding sitei220 – 2201SubstrateUniRule annotation
Metal bindingi247 – 2471Nickel 2; via pros nitrogenUniRule annotation
Metal bindingi273 – 2731Nickel 2; via tele nitrogenUniRule annotation
Active sitei321 – 3211Proton donorUniRule annotation
Metal bindingi361 – 3611Nickel 1UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-2068-MONOMER.
UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
Ordered Locus Names:RSc2032
ORF Names:RS02789
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
Proteomesi
  • UP000001436 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Urease subunit alphaPRO_0000234176Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei218 – 2181N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi267608.RSc2032.

Structurei

3D structure databases

ProteinModelPortaliQ8XXT1.
SMRiQ8XXT1. Positions 5-572.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini130 – 572443UreaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.
OrthoDBiEOG6ND0GM.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8XXT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLKITRRAY AEMFGPTTGD RVRLADTDLI VEVERDYTIY GEEVKFGGGK
60 70 80 90 100
VIRDGMGQSQ RESKDCADTV ITNALIIDHW GIVKADIGLK HGRIAAIGKA
110 120 130 140 150
GNPDIQPGVT IVIGPGTEII AGEGMIVTAG GVDTHIHFIC PQQIDEALNS
160 170 180 190 200
GVTTMIGGGT GPATGTYATT CTPGPWHLQR MLQAADAYPM NIGFLGKGNG
210 220 230 240 250
SLPGALREQI DAGAIGLKLH EDWGSTPAAI DCCLGVADDT DTQVAIHTDT
260 270 280 290 300
LNESGFVEAT IAAFKGRTIH TYHTEGAGGG HAPDIIRVCG ESNVLPSSTN
310 320 330 340 350
PTRPFTVNTL DEHLDMLMVC HHLDASIAED IAFAESRIRR ETIAAEDILH
360 370 380 390 400
DLGAFSMLSS DSQAMGRVGE VILRTWQTAH KMKAQRGKLA GDPNDARGGH
410 420 430 440 450
DNFRVKRYVA KYTINPAITH GIAHEVGSIE VGKWADLVLW KPAFFGVKPS
460 470 480 490 500
LILKGGMIAS AAMGDANASI PTPQPVHYRP MFAAAGGALA RSSLSFLSQS
510 520 530 540 550
AAQAGVAERY GLAKTTAVVR GTRAVTKAQM IHNDWQPSIT VDPETYQVVA
560 570
DGMLLTCEPA EVLPMAQRYF LF
Length:572
Mass (Da):60,944
Last modified:March 1, 2002 - v1
Checksum:iF88F080386E40E67
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD15734.1.
RefSeqiWP_011001967.1. NC_003295.1.

Genome annotation databases

EnsemblBacteriaiCAD15734; CAD15734; RSc2032.
GeneIDi1220872.
KEGGirso:RSc2032.
PATRICi20262731. VBIRalSol70888_2064.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD15734.1.
RefSeqiWP_011001967.1. NC_003295.1.

3D structure databases

ProteinModelPortaliQ8XXT1.
SMRiQ8XXT1. Positions 5-572.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267608.RSc2032.

Protein family/group databases

MEROPSiM38.982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD15734; CAD15734; RSc2032.
GeneIDi1220872.
KEGGirso:RSc2032.
PATRICi20262731. VBIRalSol70888_2064.

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.
OrthoDBiEOG6ND0GM.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.
BioCyciRSOL267608:GCVU-2068-MONOMER.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GMI1000.

Entry informationi

Entry nameiURE1_RALSO
AccessioniPrimary (citable) accession number: Q8XXT1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: March 1, 2002
Last modified: November 11, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.