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Q8XXC4 (PUR2_RALSO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:RSc2191
ORF Names:RS01408
OrganismRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]
Taxonomic identifier267608 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia

Protein attributes

Sequence length422 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 422422Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151472

Regions

Domain107 – 314208ATP-grasp
Nucleotide binding133 – 19462ATP By similarity

Sites

Metal binding2841Magnesium or manganese By similarity
Metal binding2861Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8XXC4 [UniParc].

Last modified March 27, 2002. Version 1.
Checksum: 1248E0A487C7A297

FASTA42245,016
        10         20         30         40         50         60 
MKVMVVGSGG REHALAWKLA RSPKVQVVYV APGNGGTALD KRLQNVPITD PEVLAAFAER 

        70         80         90        100        110        120 
EGVHFTVVGP EAPLAAGIVD LFRAKGLRIF GPTRAAAQLE SSKDFAKAFM QRHGIPTAKY 

       130        140        150        160        170        180 
QTFGNAAEAH AYVDREGAPI VIKADGLAAG KGVVVAMTLE EAHGAIDMML ADNRLGDAGA 

       190        200        210        220        230        240 
RVVIEEFLAG EEASFIVVCD GKDVVAMATS QDHKRLLDGD AGPNTGGMGA YSPAPVVTPT 

       250        260        270        280        290        300 
LHARVLREII LPTIRGMEKD GIPYTGFLYA GLMIDADGTP KTLEFNCRMG DPETQPIMAR 

       310        320        330        340        350        360 
MKTDLFDVLD RAIDGKLDGM ELDWDRRTAL GVVMAAYNYP DTPRKGDVIT GIPKETEDSV 

       370        380        390        400        410        420 
TFHAGTTLKD GALTTSGGRV LCVVGLADTV KAAQRAAYGA VEQIQFDGAQ YRKDIGHRAI 


RR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL646052 Genomic DNA. Translation: CAD15898.1.
RefSeqNP_520312.1. NC_003295.1.

3D structure databases

ProteinModelPortalQ8XXC4.
SMRQ8XXC4. Positions 1-422.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING267608.RSc2191.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD15898; CAD15898; RSc2191.
GeneID1221035.
KEGGrso:RSc2191.
PATRIC20263069. VBIRalSol70888_2229.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMAYKGFLYA.
OrthoDBEOG69SKD1.

Enzyme and pathway databases

BioCycRSOL267608:GCVU-2230-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_RALSO
AccessionPrimary (citable) accession number: Q8XXC4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: May 14, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways