ID   CAPP_RALN1              Reviewed;         985 AA.
AC   Q8XWW2;
DT   08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   27-MAR-2024, entry version 110.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000255|HAMAP-Rule:MF_00595};
DE            Short=PEPC {ECO:0000255|HAMAP-Rule:MF_00595};
DE            Short=PEPCase {ECO:0000255|HAMAP-Rule:MF_00595};
DE            EC=4.1.1.31 {ECO:0000255|HAMAP-Rule:MF_00595};
GN   Name=ppc {ECO:0000255|HAMAP-Rule:MF_00595}; OrderedLocusNames=RSc2358;
GN   ORFNames=RS01188;
OS   Ralstonia nicotianae (strain GMI1000) (Ralstonia solanacearum).
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
OX   NCBI_TaxID=267608;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GMI1000;
RX   PubMed=11823852; DOI=10.1038/415497a;
RA   Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M.,
RA   Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M.,
RA   Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M.,
RA   Moisan A., Robert C., Saurin W., Schiex T., Siguier P., Thebault P.,
RA   Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A.;
RT   "Genome sequence of the plant pathogen Ralstonia solanacearum.";
RL   Nature 415:497-502(2002).
CC   -!- FUNCTION: Forms oxaloacetate, a four-carbon dicarboxylic acid source
CC       for the tricarboxylic acid cycle. {ECO:0000255|HAMAP-Rule:MF_00595}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00595};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_00595};
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family. {ECO:0000255|HAMAP-
CC       Rule:MF_00595}.
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DR   EMBL; AL646052; CAD16065.1; -; Genomic_DNA.
DR   RefSeq; WP_011002281.1; NC_003295.1.
DR   AlphaFoldDB; Q8XWW2; -.
DR   SMR; Q8XWW2; -.
DR   STRING; 267608.RSc2358; -.
DR   EnsemblBacteria; CAD16065; CAD16065; RSc2358.
DR   GeneID; 60501867; -.
DR   KEGG; rso:RSc2358; -.
DR   PATRIC; fig|267608.8.peg.2397; -.
DR   eggNOG; COG2352; Bacteria.
DR   HOGENOM; CLU_006557_2_0_4; -.
DR   Proteomes; UP000001436; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation; Lyase; Magnesium.
FT   CHAIN           1..985
FT                   /note="Phosphoenolpyruvate carboxylase"
FT                   /id="PRO_0000166616"
FT   REGION          1..55
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        29..51
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        193
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
FT   ACT_SITE        634
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00595"
SQ   SEQUENCE   985 AA;  108869 MW;  B6F7B42856505B1D CRC64;
     MTQSAARRAS SRATPARKTP PAPASQTPAP SPGGTAGTAL GPTSRRSSGS AAAKDQPLKE
     DIRFLGRLLG DVLREQEGAA AFETVETIRQ TAVRFRRDGD RQAEQELDRL LKTLSRDQTT
     SVVRAFSYFS HLANIAEDQH HNRRRRVHAL AGSSPQPGSL LRALLSAADE GLSGDALRRF
     FDAALIVPVL TAHPTEVQRK SILDAQREIA RLLAERDAPL TVRERERNVT LLRAHVTKLW
     QTRMLRTTRL MVADEIENAL SYYQTTFLRE IPALYRELEE DVATVFPRRG ARGEPAPLPA
     FFQMGSWIGG DRDGNPFVTA QTLRHAAQRQ ASVILTWYLD EIHALGAELS MSTLLVDVSA
     DLLALAERSP DHSEHRADEP YRRALIGVYA RLAATCRELT GEDAGRHAVG PAPAYTRAEE
     LRADLQIVID SLAAHHGEAL ADARLASLAR AIDVFGFHLA SIDLRQVSDV HEATVAELLR
     VAGVEGAYAA LSEADKRTLL LRELQQPRLL TLPFHTYSET TASELDIFRA AREVRARYGS
     RIVRNYIISH TETLSDLLEV MLLQKEAGMF RHGTNGSGGA GLDVMVIPLF ETIEDLRNAP
     QIMGELLALP GFDAVLAAQG NEQEVMLGYS DSNKDGGFLT SNWELYKTEL ALVELFERKG
     VRLRLFHGRG GTVGRGGGPT YQAILSQPPG TVNGQIRLTE QGEIISSKFA NPEIGRRNLE
     TIVAATLEAT LLPTRNRPKG LEEFEAAMQA LSDHAFSAYR HLVYETPGFK DYFFATTPIT
     EIADLNLGSR PASRKLMDRK QRRIEDLRAI PWGFSWGQCR LLLPGWFGFG SAVQRWLDEA
     GSAKAKAARL ATLKRMHKQW PFFANLLSNM DMVLSKADLN VASRYAQLCE DRKLRNAVFS
     RISAEFTLTE QVLGAITGQS ERLADNPLLA RSIKNRFPYL DPLNHLQVEL LKRFRSGKAG
     SNDARVRRGI HLSINGIAAG LRNSG
//