Reviewed, UniProtKB/Swiss-Prot Q8XWN8 (ARGD_RALSO)

Names and origin

Protein names

Recommended name:
    Acetylornithine aminotransferase
      Short name=ACOAT
    EC=2.6.1.11

Gene names

Name:	argD
Ordered Locus Names:	RSc2435
ORF Names:	RS02668

Organism

Ralstonia solanacearum (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate. HAMAP MF_01107
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity. HAMAP MF_01107
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4. HAMAP MF_01107
Subunit structure	Homodimer By similarity. HAMAP MF_01107
Subcellular location	Cytoplasm Probable HAMAP MF_01107.
Miscellaneous	May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis. HAMAP MF_01107
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Acetylornithine aminotransferase HAMAP MF_01107		PRO_0000112772
Regions
	Region	223 – 226	4	Pyridoxal phosphate binding By similarity
Sites
	Binding site	138	1	Pyridoxal phosphate; via carbonyl oxygen By similarity
	Binding site	141	1	N(2)-acetyl-L-ornithine By similarity
	Binding site	280	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	252	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XWN8-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: D1D6C190E8351FAC	FASTA	399	42,778
	10 20 30 40 50 60 MAFADYPVQS LMYITNRPEI VFTEGKGSWL TDHNGKRYLD FVQGWAVNCL GHSNDGMIEA 70 80 90 100 110 120 LNAQAKKLIN PSPAFYNEPM AKLAGLLSAH SCFDKVFFAN SGAEANEGAI KLARKWGKKH 130 140 150 160 170 180 KSGAGKNRFE IITFDHSFHG RTLATMSASG KAGWDTIFAP QVPGFPKAIL NDIASVEALI 190 200 210 220 230 240 TDETVGVMLE PVQGEGGVLP ATQEFMQQLR ALTRKHKLLL IVDEVQAGCG RCGTLFAYQL 250 260 270 280 290 300 SNIEPDIMTL GKGIGGGVPL SALLCTDEVA SFEAGDQGGT YNGNPLMTAV GCSVIEQLLA 310 320 330 340 350 360 PGFLAGVQER GAYLRAQLLE LSEAFGLAGE RGEGLLRALL LGKDIGGQLV EAAREMNPTG 370 380 390 LLLNAPRPNI LRFMPALNVT TDEIDTMIGM LRTLLKAHG

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646052 Genomic DNA. Translation: CAD16142.1.
RefSeq	NP_520556.1.
3D structure databases
SMR	Q8XWN8. Positions 18-395.
ModBase	Search...
Genome annotation databases
GeneID	1221282.
GenomeReviews	Gene locus RSc2435 in contig AL646052_GR.
KEGG	rso:RSc2435.
NMPDR	fig|267608.1.peg.2435.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG725944.
OMA	LFAYELS.
Enzyme and pathway databases
BioCyc	RSOL267608:RSC2435-MONOMER.
BRENDA	2.6.1.11. 97066.
Family and domain databases
HAMAP	MF_01107. ArgD_aminotrans_3. [Tree]
InterPro	IPR004636. AcOrn/succinylOrn_aminoTrfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11986. Aminotrans_3. 1 hit. PTHR11986:SF19. ArgD_aminotrans. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGD_RALSO

Accession

Primary (citable) accession number: Q8XWN8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	March 1, 2002
Last modified:	January 19, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)