Reviewed, UniProtKB/Swiss-Prot Q8XWM7 (NADB1_RALSO)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase 1
      Short name=LASPO 1
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B 1

Gene names

Name:	nadB1
Ordered Locus Names:	RSc2447
ORF Names:	RS01159

Organism

Ralstonia solanacearum (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	533 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + O2 = iminosuccinate + H2O2.
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 533	533	L-aspartate oxidase 1		PRO_0000184394
Regions
	Nucleotide binding	6 – 20	15	FAD Potential
Sites
	Active site	235	1	By similarity
	Active site	254	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XWM7-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: D1D53EE6104DB7AD	FASTA	533	58,453
	10 20 30 40 50 60 MNFDVAVVGS GLAGLTVALH LADHRRVVVI SKRTLPEGAS DWAQGGIAAV LDSNDSHDEH 70 80 90 100 110 120 VDDTLIAGAG LCDEAATRYI VENGRAAIEW LIGHGVPFTR DARAELGFHL TREGGHRHRR 130 140 150 160 170 180 IIHAADATGH AVVTTLVDKV RAHPNITLLE DHFAIDLVTD AKLGLPGMRC HGLYVLDCKR 190 200 210 220 230 240 GDVKTIIASQ TVLATGGAGK VYLYTTNPDT ATGDGIAMAW RAGCRVANME FIQFHPTCLY 250 260 270 280 290 300 HPFAKSFLIS EAVRGEGGKL VLPDGTRFMP AHDERAELAP RDIVARAIDF EMKKRGLDCV 310 320 330 340 350 360 YLDISHQSPA FIQEHFPTIL ARCLELGIDI TRQPIPVVPA AHYTCGGVVT DQLGRTDIAG 370 380 390 400 410 420 LYAVGETAYT GLHGANRLAS NSLLECMVIG RGAAQDILGQ PATAPTPTPI PAWDESRVTD 430 440 450 460 470 480 ADEEVVVSHN WDELRRMMWN YVGIVRTNKR LERAQHRIAL LREEIAEYYA NFRVSHDLLE 490 500 510 520 530 LRNLVEAASL IVDSALSRHE SRGLHFSRDY PQTLPKALPT VMQPAHRRTS RKH

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL646052 Genomic DNA. Translation: CAD16154.1.
RefSeq	NP_520568.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHU based on UniProtKB P10902.
ModBase	Search...
Genome annotation databases
GeneID	1221294.
GenomeReviews	Gene locus RSc2447 in contig AL646052_GR.
KEGG	rso:RSc2447.
NMPDR	fig|267608.1.peg.2447.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8XWM7.
OMA	GAYIWNR.
Enzyme and pathway databases
BioCyc	RSOL267608:RSC2447-MON.
BRENDA	1.4.3.16. 97066.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR005288. NadB. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00551. nadB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB1_RALSO

Accession

Primary (citable) accession number: Q8XWM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)