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Protein

Argininosuccinate synthase

Gene

argG

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei43 – 431ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei99 – 991CitrullineBy similarity
Binding sitei129 – 1291ATP; via amide nitrogenBy similarity
Binding sitei131 – 1311AspartateBy similarity
Binding sitei131 – 1311ATPBy similarity
Binding sitei135 – 1351AspartateBy similarity
Binding sitei135 – 1351CitrullineBy similarity
Binding sitei136 – 1361AspartateBy similarity
Binding sitei136 – 1361ATPBy similarity
Binding sitei139 – 1391CitrullineBy similarity
Binding sitei192 – 1921CitrullineBy similarity
Binding sitei194 – 1941ATPBy similarity
Binding sitei201 – 2011CitrullineBy similarity
Binding sitei203 – 2031CitrullineBy similarity
Binding sitei280 – 2801CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 259ATPBy similarity

GO - Molecular functioni

  1. argininosuccinate synthase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-2595-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:argG
Ordered Locus Names:RSc2553
ORF Names:RS00732
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000001436: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445Argininosuccinate synthasePRO_0000148704Add
BLAST

Proteomic databases

PRIDEiQ8XWC1.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi267608.RSc2553.

Structurei

3D structure databases

ProteinModelPortaliQ8XWC1.
SMRiQ8XWC1. Positions 3-440.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230094.
KOiK01940.
OMAiMRNLDIA.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8XWC1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
METILQHVPV GQKVGIAFSG GLDTSAALRW MKNKGALPYA YTANLGQPDE
60 70 80 90 100
EDYDAIPRKA LEYGAEKARL IDCRPQLANE GIAAIQAGAF HISTGGITYF
110 120 130 140 150
NTTPLGRAVT GTMLVAAMKE DDVHIWGDGS TFKGNDIERF YRYGLLTNPA
160 170 180 190 200
LKIYKPWLDQ TFIDELGGRA EMSAFMTKEG FGYKMSAEKA YSTDSNMLGA
210 220 230 240 250
THEAKDLEHL NSGIRIVNPI MGVAFWKPEV EVKAEEVSIT FDEGRPVAVN
260 270 280 290 300
GREIADPVEM FLELNRIGGR HGLGMSDQIE NRIIEAKSRG IYEAPGMALL
310 320 330 340 350
HIAYERLVTG IHNEDTIEQY RINGLRLGRL LYQGRWFDPQ AIMLRETAQR
360 370 380 390 400
WVARAVTGTV TLELRRGNDY SILNTESPNL TYAPERLSME KVEDAPFSPA
410 420 430 440
DRIGQLTMRN LDLMDTRDKL AIYSKAGLLS LGTSSALPQL GGDKK
Length:445
Mass (Da):49,300
Last modified:March 1, 2002 - v1
Checksum:i1F73F180ED2543B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD16260.1.
RefSeqiNP_520674.1. NC_003295.1.

Genome annotation databases

EnsemblBacteriaiCAD16260; CAD16260; RSc2553.
GeneIDi1221400.
KEGGirso:RSc2553.
PATRICi20263809. VBIRalSol70888_2596.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD16260.1.
RefSeqiNP_520674.1. NC_003295.1.

3D structure databases

ProteinModelPortaliQ8XWC1.
SMRiQ8XWC1. Positions 3-440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267608.RSc2553.

Proteomic databases

PRIDEiQ8XWC1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD16260; CAD16260; RSc2553.
GeneIDi1221400.
KEGGirso:RSc2553.
PATRICi20263809. VBIRalSol70888_2596.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230094.
KOiK01940.
OMAiMRNLDIA.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciRSOL267608:GCVU-2595-MONOMER.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GMI1000.

Entry informationi

Entry nameiASSY_RALSO
AccessioniPrimary (citable) accession number: Q8XWC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: March 1, 2002
Last modified: January 7, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.