Q8XWC0 (OTC_RALSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ornithine carbamoyltransferase
Short name=OTCase
EC=2.1.3.3

Gene names

Name:	argF
Ordered Locus Names:	RSc2554
ORF Names:	RS00733

Organism

Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

267608 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP MF_01109
Subcellular location	Cytoplasm Probable HAMAP MF_01109.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Ornithine carbamoyltransferase HAMAP MF_01109		PRO_0000112995
Regions
	Region	57 – 61	5	Carbamoyl phosphate binding By similarity
	Region	263 – 266	4	Ornithine binding By similarity
Sites
	Binding site	108	1	Carbamoyl phosphate By similarity
	Binding site	135	1	Carbamoyl phosphate By similarity
	Site	32	1	Important for structural integrity By similarity
	Site	148	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XWC0 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 2666D4214824A24A	FASTA	308	35,338
	10 20 30 40 50 60 MNPPSKIKHY LQFKDFSLEE YAYLLDRSRI LKAKFKNYET WHPLHDRTLA MIFEKNSTRT 70 80 90 100 110 120 RLSFEAGIHQ LGGHAVFLNT RDSQLGRGEP IEDAAQVISR MTDIIMIRTF GQEIIERFAT 130 140 150 160 170 180 HSRVPVINGL TNEYHPCQVL ADIFTFIEHR GPIQGKTVTW VGDANNMAYT WIQAAEILGF 190 200 210 220 230 240 RFHFSAPKGY QLDPVMVADS SRPFVHVFEN PLEACEGAHL VTTDVWTSMG YEAENEARKK 250 260 270 280 290 300 AFGDWMVTEA MMQRAQPDAL FMHCLPAHRG EEVEAAVIDG KQSVVWDEAE NRLHVQKALM EYLLCGRY

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646052 Genomic DNA. Translation: CAD16261.1.
RefSeq	NP_520675.1. NC_003295.1.
3D structure databases
ProteinModelPortal	Q8XWC0.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1221401.
GenomeReviews	Gene locus RSc2554 in contig AL646052_GR.
KEGG	rso:RSc2554.
NMPDR	fig|267608.1.peg.2554.
PATRIC	20263811. VBIRalSol70888_2597.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG579429.
OMA	GVDCVVT.
ProtClustDB	PRK00779.
Enzyme and pathway databases
BioCyc	RSOL267608:RSC2554-MONOMER.
Family and domain databases
HAMAP	MF_01109. OTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024904. Orn_carbamltrans. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC_RALSO

Accession

Primary (citable) accession number: Q8XWC0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program