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Q8XW25 (NADK_RALSO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NAD kinase

EC=2.7.1.23
Alternative name(s):
ATP-dependent NAD kinase
Gene names
Name:nadK
Ordered Locus Names:RSc2650
ORF Names:RS04566
OrganismRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]
Taxonomic identifier267608 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia

Protein attributes

Sequence length302 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP By similarity. HAMAP-Rule MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361

Cofactor

Divalent metal ions By similarity. HAMAP-Rule MF_00361

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00361.

Sequence similarities

Belongs to the NAD kinase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
NAD
NADP
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processNAD metabolic process

Inferred from electronic annotation. Source: InterPro

NADP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

NAD+ kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 302302NAD kinase HAMAP-Rule MF_00361
PRO_0000120649

Regions

Nucleotide binding79 – 802NAD By similarity
Nucleotide binding153 – 1542NAD By similarity
Nucleotide binding194 – 1996NAD By similarity

Sites

Active site791Proton acceptor By similarity
Binding site1811NAD By similarity
Binding site1831NAD By similarity
Binding site2181NAD; via carbonyl oxygen By similarity
Binding site2521NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8XW25 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 1C4ADC5D6402CE5F

FASTA30232,528
        10         20         30         40         50         60 
MTSRANATSP FKTVALVGRY SAANIAAPLL ELASCIAARG HDIVFERETA LNIGVQDYPA 

        70         80         90        100        110        120 
LPPDEMARHA DVAVVLGGDG TLLGIGRHLA GASVPVIGVN HGRLGFMTDI PFEDVHNVLP 

       130        140        150        160        170        180 
DMLAGQYEAE TRSLLQAQVV RDDETIFSAL AFNDVVVNRS GFSGMVELAV SVDGFFMYNQ 

       190        200        210        220        230        240 
RSDGLIVSTP TGSTAYALSA GGPILHPALS GLVLVPIAPH ALSNRPIVIP HDAEVVIQVT 

       250        260        270        280        290        300 
SGRDASVNFD MQSLTSLLPG DRIVVRRSER TVRLLHPVGY NYYATLRKKL HWHEYPTEDN 


RL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL646052 Genomic DNA. Translation: CAD16357.1.
RefSeqNP_520771.1. NC_003295.1.

3D structure databases

ProteinModelPortalQ8XW25.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING267608.RSc2650.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD16357; CAD16357; RSc2650.
GeneID1221497.
KEGGrso:RSc2650.
PATRIC20263999. VBIRalSol70888_2691.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0061.
HOGENOMHOG000227221.
KOK00858.
OMATHEMLYH.
OrthoDBEOG6PZXDR.

Enzyme and pathway databases

BioCycRSOL267608:GCVU-2692-MONOMER.

Family and domain databases

Gene3D2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPMF_00361. NAD_kinase.
InterProIPR017438. ATP-NAD_kinase_dom_1.
IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. PolyP/ATP_NADK.
[Graphical view]
PANTHERPTHR20275. PTHR20275. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. SSF111331. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNADK_RALSO
AccessionPrimary (citable) accession number: Q8XW25
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families