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Q8XVT3 (SYL_RALSO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:RSc2744
ORF Names:RS00110
OrganismRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]
Taxonomic identifier267608 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia

Protein attributes

Sequence length877 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 877877Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152069

Regions

Motif48 – 5811"HIGH" region HAMAP-Rule MF_00049
Motif636 – 6405"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6391ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8XVT3 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 16DAD3713614475F

FASTA87797,368
        10         20         30         40         50         60 
MQDKYSPSEV EQQAQQHWQA QDAYRVTEHA RAADGSDKPK FYACSMLPYP SGKLHMGHVR 

        70         80         90        100        110        120 
NYTINDVMMR QLRMKGYNVL MPMGWDAFGM PAENAALNNG VAPAAWTYDN IAYMKKQMQS 

       130        140        150        160        170        180 
MGLAIDWSRE VATCSPDYYK WNQWLFLKML EKGIAYRKTG TVNWDPIDQT VLANEQVIDG 

       190        200        210        220        230        240 
RGWRSGAVVE KREIPMYYLR ITDYAQELLS DLDPLGWPER VKLMQQNWIG KSEGVRFAFP 

       250        260        270        280        290        300 
HGIPGDDGKV IGDGKLYVFT TRADTIMGVT FCAVAAEHPI AAHAAQGNPA LAAFIDECKH 

       310        320        330        340        350        360 
GSVMEADMAT MEKKGMPTGL TVTHPLTGEA VPVWVGNYVL MTYGDGAVMG VPAHDERDFA 

       370        380        390        400        410        420 
FANKYGLAIK QVIDVKGQPF GTEAWQEWYA DKERGVLVHS GKYDGLDYRQ AVDAVAADLA 

       430        440        450        460        470        480 
AQGLGEKKTT WRLRDWGISR QRYWGTPIPL IHCDSCGVVP VPEQDLPVRL PEDLVPDGSG 

       490        500        510        520        530        540 
NPLAKDARFL ECTCPSCGKP ARRETDTMDT FIDSCWYYMR YTCPDGATMV DGRNDYWMPM 

       550        560        570        580        590        600 
DQYIGGIEHA ILHLLYARFW TKVMRDLGLV KFDEPFTKLL TQGMVLNETY YREDAAGKKQ 

       610        620        630        640        650        660 
WINPADVDVQ TDDRGRPIGA TLKADGQPVV IGGVEKMSKS KNNGIDPQAL IDQYGADTAR 

       670        680        690        700        710        720 
LFTMFAAPPE QQLEWSDAGV EGASRFLRRA WNFGVAHAES IRAGHGNGVV NGATDADRAL 

       730        740        750        760        770        780 
RRELHTVLKQ ANYDYERLQY NTVVSAAMKM LNALEGAKDA GADARREGLG ILLRVLYPVV 

       790        800        810        820        830        840 
PHITHVLWQE LGYAGAYGGL LDAPWPQVDE GALVQSEIEL VLQVNGKVRG SIVVPADADR 

       850        860        870 
AAIEAIAAKD EAVHRFAEGK PPKKIIVVPG RLVNVVA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL646052 Genomic DNA. Translation: CAD16451.1.
RefSeqNP_520865.1. NC_003295.1.

3D structure databases

ProteinModelPortalQ8XVT3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING267608.RSc2744.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD16451; CAD16451; RSc2744.
GeneID1221591.
KEGGrso:RSc2744.
PATRIC20264199. VBIRalSol70888_2791.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycRSOL267608:GCVU-2786-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_RALSO
AccessionPrimary (citable) accession number: Q8XVT3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: March 1, 2002
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries