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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei639 – 6391ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-2786-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:RSc2744
ORF Names:RS00110
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000001436 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 877877Leucine--tRNA ligasePRO_0000152069Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi267608.RSc2744.

Structurei

3D structure databases

ProteinModelPortaliQ8XVT3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi636 – 6405"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8XVT3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDKYSPSEV EQQAQQHWQA QDAYRVTEHA RAADGSDKPK FYACSMLPYP
60 70 80 90 100
SGKLHMGHVR NYTINDVMMR QLRMKGYNVL MPMGWDAFGM PAENAALNNG
110 120 130 140 150
VAPAAWTYDN IAYMKKQMQS MGLAIDWSRE VATCSPDYYK WNQWLFLKML
160 170 180 190 200
EKGIAYRKTG TVNWDPIDQT VLANEQVIDG RGWRSGAVVE KREIPMYYLR
210 220 230 240 250
ITDYAQELLS DLDPLGWPER VKLMQQNWIG KSEGVRFAFP HGIPGDDGKV
260 270 280 290 300
IGDGKLYVFT TRADTIMGVT FCAVAAEHPI AAHAAQGNPA LAAFIDECKH
310 320 330 340 350
GSVMEADMAT MEKKGMPTGL TVTHPLTGEA VPVWVGNYVL MTYGDGAVMG
360 370 380 390 400
VPAHDERDFA FANKYGLAIK QVIDVKGQPF GTEAWQEWYA DKERGVLVHS
410 420 430 440 450
GKYDGLDYRQ AVDAVAADLA AQGLGEKKTT WRLRDWGISR QRYWGTPIPL
460 470 480 490 500
IHCDSCGVVP VPEQDLPVRL PEDLVPDGSG NPLAKDARFL ECTCPSCGKP
510 520 530 540 550
ARRETDTMDT FIDSCWYYMR YTCPDGATMV DGRNDYWMPM DQYIGGIEHA
560 570 580 590 600
ILHLLYARFW TKVMRDLGLV KFDEPFTKLL TQGMVLNETY YREDAAGKKQ
610 620 630 640 650
WINPADVDVQ TDDRGRPIGA TLKADGQPVV IGGVEKMSKS KNNGIDPQAL
660 670 680 690 700
IDQYGADTAR LFTMFAAPPE QQLEWSDAGV EGASRFLRRA WNFGVAHAES
710 720 730 740 750
IRAGHGNGVV NGATDADRAL RRELHTVLKQ ANYDYERLQY NTVVSAAMKM
760 770 780 790 800
LNALEGAKDA GADARREGLG ILLRVLYPVV PHITHVLWQE LGYAGAYGGL
810 820 830 840 850
LDAPWPQVDE GALVQSEIEL VLQVNGKVRG SIVVPADADR AAIEAIAAKD
860 870
EAVHRFAEGK PPKKIIVVPG RLVNVVA
Length:877
Mass (Da):97,368
Last modified:March 1, 2002 - v1
Checksum:i16DAD3713614475F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD16451.1.
RefSeqiWP_011002651.1. NC_003295.1.

Genome annotation databases

EnsemblBacteriaiCAD16451; CAD16451; RSc2744.
GeneIDi1221591.
KEGGirso:RSc2744.
PATRICi20264199. VBIRalSol70888_2791.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD16451.1.
RefSeqiWP_011002651.1. NC_003295.1.

3D structure databases

ProteinModelPortaliQ8XVT3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267608.RSc2744.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD16451; CAD16451; RSc2744.
GeneIDi1221591.
KEGGirso:RSc2744.
PATRICi20264199. VBIRalSol70888_2791.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-2786-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GMI1000.

Entry informationi

Entry nameiSYL_RALSO
AccessioniPrimary (citable) accession number: Q8XVT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: March 1, 2002
Last modified: July 22, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.