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Protein

Shikimate kinase

Gene

aroK

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.UniRule annotation

Catalytic activityi

ATP + shikimate = ADP + shikimate 3-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG2), Phospho-2-dehydro-3-deoxyheptonate aldolase (aroG1)
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase 2 (aroQ2), 3-dehydroquinate dehydratase 1 (aroQ1)
  4. Shikimate dehydrogenase (NADP(+)) (aroE), Shikimate dehydrogenase (NADP(+)) (aroE2)
  5. Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi17MagnesiumUniRule annotation1
Binding sitei35SubstrateUniRule annotation1
Binding sitei59SubstrateUniRule annotation1
Binding sitei81Substrate; via amide nitrogenUniRule annotation1
Binding sitei119ATPUniRule annotation1
Binding sitei138SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 18ATPUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00053; UER00088.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate kinaseUniRule annotation (EC:2.7.1.71UniRule annotation)
Short name:
SKUniRule annotation
Gene namesi
Name:aroKUniRule annotation
Ordered Locus Names:RSc2970
ORF Names:RS01327
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
Proteomesi
  • UP000001436 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002379181 – 190Shikimate kinaseAdd BLAST190

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi267608.RSc2970.

Structurei

3D structure databases

ProteinModelPortaliQ8XV61.
SMRiQ8XV61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the shikimate kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105KHV. Bacteria.
COG0703. LUCA.
HOGENOMiHOG000032568.
KOiK00891.
OMAiHGEPHFR.
OrthoDBiPOG091H02J5.

Family and domain databases

CDDicd00464. SK. 1 hit.
HAMAPiMF_00109. Shikimate_kinase. 1 hit.
InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR031322. Shikimate/glucono_kinase.
IPR000623. Shikimate_kinase/TSH1.
IPR023000. Shikimate_kinase_CS.
PfamiView protein in Pfam
PF01202. SKI. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS01128. SHIKIMATE_KINASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8XV61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSNVFFVG LMGAGKTTVG RAVARRLDLP FFDSDHEIEA RCGVRVPVIF
60 70 80 90 100
EHEGEMGFRD RETQMIDELT ARHGVVVATG GGAVLRPENR AFLRERGTVI
110 120 130 140 150
YLRANPHDLY LRTRHDKNRP LLQTENPRAR LEELHAIRDP LYREVAHFVI
160 170 180 190
ETGKPTVAQL VNMVLMQLEV AGIVVPPAAS SPTSQVSRQS
Length:190
Mass (Da):21,220
Last modified:March 1, 2002 - v1
Checksum:i3D1754E5398B66F6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646052 Genomic DNA. Translation: CAD16679.1.

Genome annotation databases

EnsemblBacteriaiCAD16679; CAD16679; RSc2970.
KEGGirso:RSc2970.

Similar proteinsi

Entry informationi

Entry nameiAROK_RALSO
AccessioniPrimary (citable) accession number: Q8XV61
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: March 1, 2002
Last modified: June 7, 2017
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families