Reviewed, UniProtKB/Swiss-Prot Q8XU39 (PH4H_RALSO)

Names and origin

Protein names

Recommended name:
    Phenylalanine-4-hydroxylase
      Short name=PAH
    EC=1.14.16.1
Alternative name(s):
    Phe-4-monooxygenase

Gene names

Name:	phhA
Ordered Locus Names:	RSc3355
ORF Names:	RS02630

Organism

Ralstonia solanacearum (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-phenylalanine + tetrahydrobiopterin + O2 = L-tyrosine + 4a-hydroxytetrahydrobiopterin.
Cofactor	Binds 1 Fe2+ ion By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 1/6.
Sequence similarities	Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism
Ligand	Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine 4-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Phenylalanine-4-hydroxylase		PRO_0000205556
Sites
	Metal binding	154	1	Iron Potential
	Metal binding	159	1	Iron Potential
	Metal binding	200	1	Iron Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8XU39-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 2EBA9E216476371B	FASTA	313	34,901
	10 20 30 40 50 60 MAIATPTSAA PTPAPAGFTG TLTDKLREQF AEGLDGQTLR PDFTMEQPVH RYTAADHATW 70 80 90 100 110 120 RTLYDRQEAL LPGRACDEFL QGLSTLGMSR EGVPSFDRLN ETLMRATGWQ IVAVPGLVPD 130 140 150 160 170 180 EVFFEHLANR RFPASWWMRR PDQLDYLQEP DGFHDIFGHV PLLINPVFAD YMQAYGQGGL 190 200 210 220 230 240 KAARLGALDM LARLYWYTVE FGLIRTPAGL RIYGAGIVSS KSESVYALDS ASPNRIGFDV 250 260 270 280 290 300 HRIMRTRYRI DTFQKTYFVI DSFEQLFDAT RPDFTPLYEA LGTLPTFGAG DVVDGDAVLN 310 AGTREGWADT ADI

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646052 Genomic DNA. Translation: CAD17143.1.
RefSeq	NP_521474.1.
3D structure databases
SMR	Q8XU39. Positions 15-271, 42-301.
ModBase	Search...
Genome annotation databases
GeneID	1222219.
GenomeReviews	Gene locus RSc3355 in contig AL646052_GR.
KEGG	rso:RSc3355.
NMPDR	fig|267608.1.peg.3353.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG403840.
OMA	FLARLYW.
Enzyme and pathway databases
BioCyc	RSOL267608:RSC3355-MONOMER.
BRENDA	1.14.16.1. 97066.
Family and domain databases
InterPro	IPR001273. ArAA_hydroxylase. IPR018301. ArAA_hydroxylase_Fe/CU_BS. IPR019774. Aromatic-AA_hydroxylase_C. IPR005960. Phe-4-hydroxylase_mono. [Graphical view]
Gene3D	G3DSA:1.10.800.10. Aaa_hydroxylase. 1 hit.
PANTHER	PTHR11473. Aaa_hydroxylase. 1 hit.
Pfam	PF00351. Biopterin_H. 1 hit. [Graphical view]
PRINTS	PR00372. FYWHYDRXLASE.
TIGRFAMs	TIGR01267. Phe4hydrox_mono. 1 hit.
PROSITE	PS00367. BIOPTERIN_HYDROXYL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PH4H_RALSO

Accession

Primary (citable) accession number: Q8XU39

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)