Pyridoxal-phosphate-dependent serine hydroxymethyltransferase 2 - Ralstonia solanacearum (strain GMI1000)

Contribute

Read comments (?) or add your own

Q8XTQ1 (GLYA2_RALSO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 66. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

to top of page

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

to top of page

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Pyridoxal-phosphate-dependent serine hydroxymethyltransferase 2
Short name=SHMT 2
Short name=Serine methylase 2
EC=2.1.2.1

Gene names

Name:	glyA2
Ordered Locus Names:	RSp0055
ORF Names:	RS02030

Encoded on

Plasmid megaplasmid Rsp

Organism

Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate serving as the one-carbon carrier By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H₂O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00051
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051 Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00051.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

424

Pyridoxal-phosphate-dependent serine hydroxymethyltransferase 2

PRO_0000113645

Regions

Region

3

Substrate binding By similarity

Sites

Binding site

1

Pyridoxal phosphate By similarity

Binding site

1

Pyridoxal phosphate By similarity

Binding site

1

Substrate By similarity

Binding site

1

Substrate binding By similarity

Binding site

1

Pyridoxal phosphate By similarity

Binding site

1

Substrate By similarity

Binding site

1

Pyridoxal phosphate By similarity

Binding site

1

Pyridoxal phosphate By similarity

Binding site

1

Pyridoxal phosphate By similarity

Binding site

1

Pyridoxal phosphate By similarity

Binding site

1

Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity

Binding site

1

Pyridoxal phosphate By similarity

Amino acid modifications

Modified residue

1

N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q8XTQ1 [UniParc].

Last modified March 27, 2002. Version 1.
Checksum: 8C362B853086A35A
Show »

424

45,667

        10         20         30         40         50         60 
MSNTQSFFSQ SLAERDAPIR SSLLKELERQ QSQVELIASE NIVSRAVLEA QGSVLTNKYA 

        70         80         90        100        110        120 
EGYPGKRYYG GCEYADEVES LAIDRVKQLF NAGFANVQPH SGAQANGAVM LALTKPGDTV 

       130        140        150        160        170        180 
LGMSLDAGGH LTHGAKPALS GKWFNAMQYG VNRDTMLIDY EQVEKLAQEH KPSLIIAGFS 

       190        200        210        220        230        240 
AYPRKLDFAR FRAIADSVGA KLMVDMAHIA GVIAAGRHDN PVDHAHVVTS TTHKTLRGPR 

       250        260        270        280        290        300 
GGFVLTNDEE IAKKINSAVF PGLQGGPLMH VIAGKAVAFG EALKPEFKTY IDSVLANAKA 

       310        320        330        340        350        360 
LGEVLKAGGV DLVTGGTDNH LLLVDLRPKG LKGTQVEQAL ERAGITCNKN GIPFDTEKPT 

       370        380        390        400        410        420 
ITSGIRLGTP AATTRGFGVA EFEQIGRLIL EVFEALRANP DGDRATEHRV RSEIFALCDR 


FPIY

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum."
Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A.

expand/collapse author list

Boucher C.A.
Nature 415:497-502(2002) [PubMed: 11823852] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: GMI1000.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646053 Genomic DNA. Translation: CAD17206.1.
RefSeq	NP_521616.1. NC_003296.1.
3D structure databases
ProteinModelPortal	Q8XTQ1.
SMR	Q8XTQ1. Positions 14-389.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1222603.
GenomeReviews	Gene locus RSp0055 in contig AL646053_GR.
KEGG	rso:RSp0055.
NMPDR	fig\|267608.1.peg.3495.
PATRIC	20265731. VBIRalSol70888_3538.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG301263.
OMA	SHRIDMD.
ProtClustDB	PRK13034.
Enzyme and pathway databases
BioCyc	RSOL267608:RSP0055-MONOMER.
Family and domain databases
HAMAP	MF_00051. SHMT. [Tree]
InterPro	IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K00600.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA2_RALSO

Accession

Primary (citable) accession number: Q8XTQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	March 27, 2002
Last modified:	January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of page

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents