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Q8XT76 (GLGB_RALSO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:RSp0239
ORF Names:RS06097
Encoded onPlasmid megaplasmid Rsp
OrganismRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]
Taxonomic identifier267608 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia

Protein attributes

Sequence length775 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7757751,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188733

Sites

Active site4541Nucleophile By similarity
Active site5071Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8XT76 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: F6A3496E6A56D56F

FASTA77585,728
        10         20         30         40         50         60 
MTSVHDFATA TRPATPSAAA QEPAPALPPG LDRNTLDALT HGRLGDPFAV LGPHRLETAE 

        70         80         90        100        110        120 
GERPHRVVRA FHPGARRVQA IDPRGQVMAE LAPVGRTGLF HGRLPDDAPD ADGHPGAYRL 

       130        140        150        160        170        180 
RVVWPAGAGH EAVQEAEDPY AFGLLLGDLD LHLIAEGRHW ELARCLGAQA MRQDDVAGVR 

       190        200        210        220        230        240 
FAVWAPNARR VSVVGDFNQW DGRRHPMRLR HGTGVWELFV PAGLGAGPGS RYKFELVGAD 

       250        260        270        280        290        300 
GHLLVKADPV ARRTEAPPAT ASVVADPLPF RWSDAAWMET RAARQRPDAP IAIYEVHAGS 

       310        320        330        340        350        360 
WLRDVEDGGR SLDWDALGER LIPYVAGLGF THVELLPVAE HPFGGSWGYQ PLGLFAPSAR 

       370        380        390        400        410        420 
FGPPEAFARF VERCHQAALG VIVDWVPAHF PSDPHGLARF DGTALYEHAD PREGFHQDWN 

       430        440        450        460        470        480 
TLIYNFGRHE VRGFLIASAL EWLEHFHIDG LRVDAVASML YRDYSRPADA WVPNRYGGRE 

       490        500        510        520        530        540 
NLEAVAFLQQ MNAVVHARCP GAITIAEEST AWPGVTAAVE FNGLGFDYKW NMGWMHDTLH 

       550        560        570        580        590        600 
YMQRDPIYRQ HHHDGLTFGL VYAFSERFIL PLSHDEVVHG KGSLLGKMPG DDWQRLANLR 

       610        620        630        640        650        660 
AYLAFMWTHP GKKLLFMGGE FGQLGEWNHD AAPEWHLLDD PRHRGVQRLV HDLNALYRSE 

       670        680        690        700        710        720 
PALHARDCAP EGFSWVIGDD RANSVFAYLR LDTAGTPMLI VANMTPVPRD GYRIGVPDVD 

       730        740        750        760        770 
GAVRWREMLN TDSAVYGGTN LGNGGVVDVE DVESHGWRRS VVVRLPPLAV VVLKV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL646053 Genomic DNA. Translation: CAD17390.1.
RefSeqNP_521800.1. NC_003296.1.

3D structure databases

ProteinModelPortalQ8XT76.
SMRQ8XT76. Positions 162-774.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING267608.RSp0239.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD17390; CAD17390; RSp0239.
GeneID1222370.
KEGGrso:RSp0239.
PATRIC20266071. VBIRalSol70888_3708.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAAKLLFMG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycRSOL267608:GCVU-3742-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_RALSO
AccessionPrimary (citable) accession number: Q8XT76
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2002
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries