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Q8XT76

- GLGB_RALSO

UniProt

Q8XT76 - GLGB_RALSO

Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei454 – 4541NucleophileUniRule annotation
    Active sitei507 – 5071Proton donorUniRule annotation

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

    Enzyme and pathway databases

    BioCyciRSOL267608:GCVU-3742-MONOMER.
    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan branching enzyme GlgBUniRule annotation (EC:2.4.1.18UniRule annotation)
    Alternative name(s):
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferaseUniRule annotation
    Alpha-(1->4)-glucan branching enzymeUniRule annotation
    Glycogen branching enzymeUniRule annotation
    Short name:
    BEUniRule annotation
    Gene namesi
    Name:glgBUniRule annotation
    Ordered Locus Names:RSp0239
    ORF Names:RS06097
    Encoded oniPlasmid megaplasmid Rsp0 Publication
    OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
    Taxonomic identifieri267608 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
    ProteomesiUP000001436: Plasmid megaplasmid Rsp

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7757751,4-alpha-glucan branching enzyme GlgBPRO_0000188733Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi267608.RSp0239.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8XT76.
    SMRiQ8XT76. Positions 162-774.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0296.
    HOGENOMiHOG000283037.
    KOiK00700.
    OMAiAKLLFMG.
    OrthoDBiEOG6JX7GT.

    Family and domain databases

    Gene3Di2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_00685. GlgB.
    InterProiIPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000463. GlgB. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 2 hits.
    TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8XT76-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTSVHDFATA TRPATPSAAA QEPAPALPPG LDRNTLDALT HGRLGDPFAV    50
    LGPHRLETAE GERPHRVVRA FHPGARRVQA IDPRGQVMAE LAPVGRTGLF 100
    HGRLPDDAPD ADGHPGAYRL RVVWPAGAGH EAVQEAEDPY AFGLLLGDLD 150
    LHLIAEGRHW ELARCLGAQA MRQDDVAGVR FAVWAPNARR VSVVGDFNQW 200
    DGRRHPMRLR HGTGVWELFV PAGLGAGPGS RYKFELVGAD GHLLVKADPV 250
    ARRTEAPPAT ASVVADPLPF RWSDAAWMET RAARQRPDAP IAIYEVHAGS 300
    WLRDVEDGGR SLDWDALGER LIPYVAGLGF THVELLPVAE HPFGGSWGYQ 350
    PLGLFAPSAR FGPPEAFARF VERCHQAALG VIVDWVPAHF PSDPHGLARF 400
    DGTALYEHAD PREGFHQDWN TLIYNFGRHE VRGFLIASAL EWLEHFHIDG 450
    LRVDAVASML YRDYSRPADA WVPNRYGGRE NLEAVAFLQQ MNAVVHARCP 500
    GAITIAEEST AWPGVTAAVE FNGLGFDYKW NMGWMHDTLH YMQRDPIYRQ 550
    HHHDGLTFGL VYAFSERFIL PLSHDEVVHG KGSLLGKMPG DDWQRLANLR 600
    AYLAFMWTHP GKKLLFMGGE FGQLGEWNHD AAPEWHLLDD PRHRGVQRLV 650
    HDLNALYRSE PALHARDCAP EGFSWVIGDD RANSVFAYLR LDTAGTPMLI 700
    VANMTPVPRD GYRIGVPDVD GAVRWREMLN TDSAVYGGTN LGNGGVVDVE 750
    DVESHGWRRS VVVRLPPLAV VVLKV 775
    Length:775
    Mass (Da):85,728
    Last modified:March 1, 2002 - v1
    Checksum:iF6A3496E6A56D56F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL646053 Genomic DNA. Translation: CAD17390.1.
    RefSeqiNP_521800.1. NC_003296.1.
    WP_011003553.1. NC_003296.1.

    Genome annotation databases

    EnsemblBacteriaiCAD17390; CAD17390; RSp0239.
    GeneIDi1222370.
    KEGGirso:RSp0239.
    PATRICi20266071. VBIRalSol70888_3708.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL646053 Genomic DNA. Translation: CAD17390.1 .
    RefSeqi NP_521800.1. NC_003296.1.
    WP_011003553.1. NC_003296.1.

    3D structure databases

    ProteinModelPortali Q8XT76.
    SMRi Q8XT76. Positions 162-774.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 267608.RSp0239.

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD17390 ; CAD17390 ; RSp0239 .
    GeneIDi 1222370.
    KEGGi rso:RSp0239.
    PATRICi 20266071. VBIRalSol70888_3708.

    Phylogenomic databases

    eggNOGi COG0296.
    HOGENOMi HOG000283037.
    KOi K00700.
    OMAi AKLLFMG.
    OrthoDBi EOG6JX7GT.

    Enzyme and pathway databases

    UniPathwayi UPA00164 .
    BioCyci RSOL267608:GCVU-3742-MONOMER.

    Family and domain databases

    Gene3Di 2.60.40.10. 2 hits.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_00685. GlgB.
    InterProi IPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000463. GlgB. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 2 hits.
    TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: GMI1000.

    Entry informationi

    Entry nameiGLGB_RALSO
    AccessioniPrimary (citable) accession number: Q8XT76
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 15, 2003
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Plasmid, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3