Q8XS05 (METE_RALSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	RSp0676
ORF Names:	RS01773

Encoded on

Plasmid megaplasmid Rsp

Organism

Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

267608 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	776 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 776	776	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_0000098651
Sites
	Metal binding	642	1	Zinc By similarity
	Metal binding	644	1	Zinc By similarity
	Metal binding	727	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XS05 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: DA9BF9A14DC2C0C8	FASTA	776	85,308
	10 20 30 40 50 60 MTTIHTLGYP RIGAQRELKF ALESFWKGAS TEADLRDTGR ALRERHWNAQ RDAGLDFVTV 70 80 90 100 110 120 GDFAWYDQVL QTAALLGALP TRYGFDPAQL TLAQSFVLAR GNADHAAMEM TKWFDTNYHY 130 140 150 160 170 180 LVPELTPDLR FGPGTGWLFD EVREAQAAGH RVKVALLGPV TFLHLAKARG GLADKLSLLP 190 200 210 220 230 240 QLLPAYAAVL KRLAAEGVEW VQIDEPALVL DLPQAWSDAY GPAYATLAAA GGPRLLLATY 250 260 270 280 290 300 FEAASHHAAL IRSLPVAGVH LDLVRAPQQL EAFAPWPADK VLSAGVVDGR NIWRTDLEQA 310 320 330 340 350 360 LARVAPLAQT LGERLWLAPS CSLLHVPVDL AAETRLDDEL KGWLAFARQK LDELAVLKRA 370 380 390 400 410 420 VIDGRDAAHA ALAGSAAAIA SRAASRRVHN DSVKKRAAAI RAQDAERAAP YPVRAAAQQA 430 440 450 460 470 480 RLNLPLLPTT TIGSFPQTAE IRQARAQYKR GELQALTYLE RMRAEIADVV QRQEALGLDM 490 500 510 520 530 540 LVHGEAERND MVEYFGELLW GYAFTANGWV QSYGSRCVKP PVIYGDVYRP EPMTVEWSRY 550 560 570 580 590 600 AQSLTAKPMK GMLTGPVTML QWSFVRDDQP REQTALQIAL ALRDEVRDLE AAGIAAIQID 610 620 630 640 650 660 EPAFREGLPL RAGDVAVYLE WAARVFRVSA SGVRNDTQIH THMCYSEFND ILPAIASMDA 670 680 690 700 710 720 DVITIETSRS NMELLDAFGE FAYPNEIGPG VYDIHSPRVP RVEEMEALLD KAAQVVPVQR 730 740 750 760 770 LWVNPDCGLK TRGWPEVEAA LRGMVEATRR LRARHAGAVH AGTPATRAEH AESALA

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646053 Genomic DNA. Translation: CAD17827.1.
RefSeq	NP_522237.1. NC_003296.1.
3D structure databases
ProteinModelPortal	Q8XS05.
SMR	Q8XS05. Positions 4-754.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1222983.
GenomeReviews	Gene locus RSp0676 in contig AL646053_GR.
KEGG	rso:RSp0676.
NMPDR	fig|267608.1.peg.4116.
PATRIC	20266955. VBIRalSol70888_4149.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	NIWANDF.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	RSOL267608:RSP0676-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_RALSO

Accession

Primary (citable) accession number: Q8XS05

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program