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Protein

Homogentisate 1,2-dioxygenase

Gene

hmgA

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the catabolism of homogentisate (2,5-dihydroxyphenylacetate or 2,5-OH-PhAc), a central intermediate in the degradation of phenylalanine and tyrosine. Catalyzes the oxidative ring cleavage of the aromatic ring of homogentisate to yield maleylacetoacetate.UniRule annotation

Catalytic activityi

Homogentisate + O2 = 4-maleylacetoacetate.UniRule annotation

Cofactori

Fe cationUniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei302 – 3021Proton acceptorUniRule annotation
Metal bindingi345 – 3451IronUniRule annotation
Metal bindingi351 – 3511IronUniRule annotation
Binding sitei360 – 3601homogentisateUniRule annotation
Metal bindingi381 – 3811IronUniRule annotation
Binding sitei381 – 3811homogentisateUniRule annotation

GO - Molecular functioni

  1. homogentisate 1,2-dioxygenase activity Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-phenylalanine catabolic process Source: UniProtKB-HAMAP
  2. tyrosine catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Phenylalanine catabolism, Tyrosine catabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciRSOL267608:GCVU-4195-MONOMER.
UniPathwayiUPA00139; UER00339.

Names & Taxonomyi

Protein namesi
Recommended name:
Homogentisate 1,2-dioxygenaseUniRule annotation (EC:1.13.11.5UniRule annotation)
Short name:
HGDOUniRule annotation
Alternative name(s):
Homogentisate oxygenaseUniRule annotation
Homogentisic acid oxidaseUniRule annotation
HomogentisicaseUniRule annotation
Gene namesi
Name:hmgAUniRule annotation
Ordered Locus Names:RSp0691
ORF Names:RS01758
Encoded oniPlasmid megaplasmid Rsp0 Publication
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
ProteomesiUP000001436: Plasmid megaplasmid Rsp

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 448448Homogentisate 1,2-dioxygenasePRO_0000220252Add
BLAST

Interactioni

Subunit structurei

Hexamer; dimer of trimers.UniRule annotation

Protein-protein interaction databases

STRINGi267608.RSp0691.

Structurei

3D structure databases

ProteinModelPortaliQ8XRZ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the homogentisate dioxygenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3508.
HOGENOMiHOG000139824.
KOiK00451.
OMAiRGYLCEN.
OrthoDBiEOG6D5FZK.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00334. Homogentis_dioxygen.
InterProiIPR005708. Homogentis_dOase.
IPR022950. Homogentis_dOase_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR11056. PTHR11056. 1 hit.
PfamiPF04209. HgmA. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01015. hmgA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8XRZ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMLAPAAKN AFTPASPDRP AYQSGFGNEF ATEALPGALP HGQNSPQQAP
60 70 80 90 100
YGLYAEQLSG TAFTAPRAHN RRAWLYRIRP AAVHLPFEPI AQDRFHSDFH
110 120 130 140 150
AVPASPNQLR WDPLPAPAAG TDFIDGIVTF AGNGGPDAQT GCGIHLYAAN
160 170 180 190 200
ASMTGRFFYN ADGELLIVPQ QGRLRLLTEL GVLDVEPLEI AVIPRGVRFR
210 220 230 240 250
VELPDGEARG YLCENFGAIF RLPDLGVIGS NGLANPRDFL TPHAWYEDRE
260 270 280 290 300
GDFELVAKFH GNLWRARIGH SPLDVVAWHG NYAPYKYDLR LFNTIGSISY
310 320 330 340 350
DHPDPSIFLV LQSVSDTPGV DAIDFVIFPP RWLAMEHSFR PPWFHRNIAS
360 370 380 390 400
EFMGLIQGVY DAKAEGFVPG GASLHNCMTG HGPDAETFEK ASHADTTQPH
410 420 430 440
KVEATMAFMF ETRGVIRPTR FAAESAQLQA RYFECWQGLK KHFDPAKR
Length:448
Mass (Da):49,537
Last modified:March 1, 2002 - v1
Checksum:i6467FE12F70996D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646053 Genomic DNA. Translation: CAD17842.1.
RefSeqiNP_522252.1. NC_003296.1.

Genome annotation databases

EnsemblBacteriaiCAD17842; CAD17842; RSp0691.
GeneIDi1222998.
KEGGirso:RSp0691.
PATRICi20266987. VBIRalSol70888_4165.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646053 Genomic DNA. Translation: CAD17842.1.
RefSeqiNP_522252.1. NC_003296.1.

3D structure databases

ProteinModelPortaliQ8XRZ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi267608.RSp0691.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAD17842; CAD17842; RSp0691.
GeneIDi1222998.
KEGGirso:RSp0691.
PATRICi20266987. VBIRalSol70888_4165.

Phylogenomic databases

eggNOGiCOG3508.
HOGENOMiHOG000139824.
KOiK00451.
OMAiRGYLCEN.
OrthoDBiEOG6D5FZK.

Enzyme and pathway databases

UniPathwayiUPA00139; UER00339.
BioCyciRSOL267608:GCVU-4195-MONOMER.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00334. Homogentis_dioxygen.
InterProiIPR005708. Homogentis_dOase.
IPR022950. Homogentis_dOase_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR11056. PTHR11056. 1 hit.
PfamiPF04209. HgmA. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01015. hmgA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: GMI1000.

Entry informationi

Entry nameiHGD_RALSO
AccessioniPrimary (citable) accession number: Q8XRZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2002
Last modified: January 7, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.