Reviewed, UniProtKB/Swiss-Prot Q8XRZ0 (HGD_RALSO)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisicase
    Homogentisate oxygenase
    Homogentisic acid oxidase

Gene names

Name:	hmgA
Ordered Locus Names:	RSp0691
ORF Names:	RS01758

Encoded on

Plasmid megaplasmid Rsp

Organism

Ralstonia solanacearum (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP MF_00334
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 4/6. HAMAP MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 448	448	Homogentisate 1,2-dioxygenase HAMAP MF_00334		PRO_0000220252
Sites
	Metal binding	345	1	Iron By similarity
	Metal binding	351	1	Iron By similarity
	Metal binding	381	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XRZ0-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 6467FE12F70996D5	FASTA	448	49,537
	10 20 30 40 50 60 MNMLAPAAKN AFTPASPDRP AYQSGFGNEF ATEALPGALP HGQNSPQQAP YGLYAEQLSG 70 80 90 100 110 120 TAFTAPRAHN RRAWLYRIRP AAVHLPFEPI AQDRFHSDFH AVPASPNQLR WDPLPAPAAG 130 140 150 160 170 180 TDFIDGIVTF AGNGGPDAQT GCGIHLYAAN ASMTGRFFYN ADGELLIVPQ QGRLRLLTEL 190 200 210 220 230 240 GVLDVEPLEI AVIPRGVRFR VELPDGEARG YLCENFGAIF RLPDLGVIGS NGLANPRDFL 250 260 270 280 290 300 TPHAWYEDRE GDFELVAKFH GNLWRARIGH SPLDVVAWHG NYAPYKYDLR LFNTIGSISY 310 320 330 340 350 360 DHPDPSIFLV LQSVSDTPGV DAIDFVIFPP RWLAMEHSFR PPWFHRNIAS EFMGLIQGVY 370 380 390 400 410 420 DAKAEGFVPG GASLHNCMTG HGPDAETFEK ASHADTTQPH KVEATMAFMF ETRGVIRPTR 430 440 FAAESAQLQA RYFECWQGLK KHFDPAKR

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL646053 Genomic DNA. Translation: CAD17842.1.
RefSeq	NP_522252.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1222998.
GenomeReviews	Gene locus RSp0691 in contig AL646053_GR.
KEGG	rso:RSp0691.
NMPDR	fig|267608.1.peg.4131.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8XRZ0.
OMA	Q8XRZ0. HRNCMSE.
Enzyme and pathway databases
BioCyc	RSOL267608:RSP0691-MON.
BRENDA	1.13.11.5. 97066.
Family and domain databases
HAMAP	MF_00334. [Tree]
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_RALSO

Accession

Primary (citable) accession number: Q8XRZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 1, 2002
Last modified:	May 5, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)