Q8XRY7 (T23O_RALSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	RSp0694

Encoded on

Plasmid megaplasmid Rsp

Organism

Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

267608 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Tryptophan 2,3-dioxygenase		PRO_0000360130
Regions
	Region	6 – 10	5	Substrate binding By similarity
	Region	36 – 40	5	Substrate binding By similarity
Sites
	Metal binding	303	1	Iron (heme axial ligand) By similarity
	Binding site	107	1	Substrate By similarity
	Binding site	114	1	Heme By similarity
	Binding site	317	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XRY7 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 5554D0579E7E3161	FASTA	369	42,166
	10 20 30 40 50 60 MKPMSYWDYI KVEELLALQG GANGDETQVG NDEALFIVVH QVYELWFKLI LRELTFARDL 70 80 90 100 110 120 LRQDTVPGHQ IALGVRSLRR AIAVFEQANQ HFRVMETMTA RDFLDFRERL MPASGFQSAQ 130 140 150 160 170 180 LREIEILLGL EDNERIAVCQ GGSFKDALKL PNGALSSAAY RVEAREAHGQ SLKHCLYAWL 190 200 210 220 230 240 SRIPIDGSNE PAAVKRFLRD YIGSVRAESQ RRLQTAIDRQ LAPAEVERLR ARYQADDVGA 250 260 270 280 290 300 ETFLLAEEDP QADAMTREKR RAVRAAMLFV ESYRELPQLA WPRELLESIL ELEQSMLIWR 310 320 330 340 350 360 QRHARMVERI IGRRVGTGGS SGVDYLDQTA LRYRVFTDLW TVRSLLLRKS SVPPIRQGAS YAFAEEALV

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646053 Genomic DNA. Translation: CAD17845.1.
RefSeq	NP_522255.1. NC_003296.1.
3D structure databases
HSSP	HSSP built from PDB template 1YW0 based on UniProtKB Q8PDA8.
ProteinModelPortal	Q8XRY7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1223001.
GenomeReviews	Gene locus RSp0694 in contig AL646053_GR.
KEGG	rso:RS01755.
NMPDR	fig|267608.1.peg.4134.
PATRIC	20266995. VBIRalSol70888_4169.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG690843.
OMA	HFRVMET.
ProtClustDB	CLSK897804.
Enzyme and pathway databases
BioCyc	RSOL267608:RSP0694-MONOMER.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23O_RALSO

Accession

Primary (citable) accession number: Q8XRY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program