Q8XRV9 (ASPD_RALSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable L-aspartate dehydrogenase
EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	RSp0722
ORF Names:	RS01727

Encoded on

Plasmid megaplasmid Rsp

Organism

Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

267608 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP MF_01265
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity. HAMAP MF_01265
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro NADP catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 268	268	Probable L-aspartate dehydrogenase HAMAP MF_01265		PRO_0000144891
Sites
	Active site	221	1	By similarity
	Binding site	125	1	NAD; via amide nitrogen By similarity
	Binding site	191	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XRV9 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 9195BAB7EAA9AAE9	FASTA	268	28,259
	10 20 30 40 50 60 MLHVSMVGCG AIGQGVLELL KSDPDLCFDT VIVPEHGMDR ARAAIAPFAP RTRVMTRLPA 70 80 90 100 110 120 QADRPDLLVE CAGHDALREH VVPALEQGID CLVVSVGALS EPGLAERLEA AARRGHAQMQ 130 140 150 160 170 180 LLSGAIGAID ALAAARVGGL DAVVYTGRKP PRAWKGTPAE RQFDLDALDR TTVIFEGKAS 190 200 210 220 230 240 DAALLFPKNA NVAATLALAG LGMERTHVRL LADPTIDENI HHVEARGAFG GFELIMRGKP 250 260 LAANPKTSAL TVFSVVRALG NRAHAVSI

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646053 Genomic DNA. Translation: CAD17873.1.
RefSeq	NP_522283.1. NC_003296.1.
3D structure databases
ProteinModelPortal	Q8XRV9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1223029.
GenomeReviews	Gene locus RSp0722 in contig AL646053_GR.
KEGG	rso:RS01727.
NMPDR	fig|267608.1.peg.4162.
PATRIC	20267051. VBIRalSol70888_4197.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG649642.
OMA	ECAGHSA.
ProtClustDB	PRK13303.
Enzyme and pathway databases
BioCyc	RSOL267608:RSP0722-MONOMER.
Family and domain databases
HAMAP	MF_01265. NadX. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR020626. Asp_DH_NAD_syn_prok. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K06989.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASPD_RALSO

Accession

Primary (citable) accession number: Q8XRV9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program