Reviewed, UniProtKB/Swiss-Prot Q8XRN1 (DNAE2_RALSO)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	RSp0800
ORF Names:	RS01907

Encoded on

Plasmid megaplasmid Rsp

Organism

Ralstonia solanacearum (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	1075 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1075	1075	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103393

Sequences

	Sequence		Length	Mass (Da)
	Q8XRN1-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 0602FCF53EDFDC60	FASTA	1,075	119,253
	10 20 30 40 50 60 MNAPTPLPIG LPDYAELHCI SNFTFLTGAS HPHELVARAQ AFGYRALALT DECSVAGTVR 70 80 90 100 110 120 AHMAAKEAGL KLIIGSRFTV RDAQGEPWLR LVLLAQDIDG YGNLCECITQ ARLAAPKGDY 130 140 150 160 170 180 RLLADDLAAP AGELAHLRGM PHCLAILLPD YDPDPRHLLA QAQWCRQVFG DRLSMALELP 190 200 210 220 230 240 LRHADDRHRG TVAAVSEQTD VPMVATGDVA MHSRQRKPLH DVLTAIRLSR PIAECGLALA 250 260 270 280 290 300 PSAEQAMRTR MQLAFFYQGE RGAQVLRRSV ELASLCDFSL DEIAYEYPHE VVPEGQTPAG 310 320 330 340 350 360 YLLAEVMAGA ARRYGKDIPS KVRAQLDEEL ALIAELRYEP FFLTVYDVVR FARSQSILCQ 370 380 390 400 410 420 GRGSAANSAV CYCLGITEVN PESGNTLFAR FLSRARNEPP DIDVDFEHQR REEVIQYIYK 430 440 450 460 470 480 KYGVTRTALA AALITYRTRS ALRDVGRALG IDLGVIEQVA KAQAWWDGRH EFAQRAGQQG 490 500 510 520 530 540 LDPESPLVQR WAGLIEQLRG FPRHLSQHVG GFVIAQGKLS RLVPIENAAM PERRVIQWDK 550 560 570 580 590 600 DDLESLRLLK VDVLALGMLT AIRRTLDMLD ALPGRRAHYG APDKLAMQHL PDEDTATYEM 610 620 630 640 650 660 ICRAETIGVF QIESRAQQSM LPRLRPRTYY DLVIQVAIVR PGPIQGGMVH PYLQRREAVR 670 680 690 700 710 720 NGNPDCVTYP GPEVKAVLER TLGVPIFQEQ VMEIAMKAGG FTADEADRLR RDMAAWKRKG 730 740 750 760 770 780 NLTQHQARLM KEMVEVRHYD PAFVEALCRQ MEGFAEYGFP ESHAAGFARL AYVSSFLKCH 790 800 810 820 830 840 EPAAFFAALL NSQPMGFYSP SQLVQEARRS RVRVLPADVT ASAWDSTLHA RPDGAGGQPD 850 860 870 880 890 900 IRLGLNRIRG MRPAAGERIV VARAQAPFSS VEDLAHRAAL DRHDLSVLAA ANALLSLAGH 910 920 930 940 950 960 RRQALWQTLA LQEPGQDHAL LRQARPVEAP LVLPAPSLGE EVVADYGSLG LSLQSHPLSL 970 980 990 1000 1010 1020 LRPRLERMRF VTAAALAGYR NGQLARACGI VTVRQRPGTA KGTIFVSIED ETGAVNVVLW 1030 1040 1050 1060 1070 PRLIERQRRE VLHARLLGVY GKWQCERETR HLVAQHLVDL TPLLGRLASS SRDFH

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646053 Genomic DNA. Translation: CAD17951.1.
RefSeq	NP_522361.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1223107.
GenomeReviews	Gene locus RSp0800 in contig AL646053_GR.
KEGG	rso:RSp0800.
NMPDR	fig|267608.1.peg.4240.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734490.
OMA	LATNRNG.
Enzyme and pathway databases
BioCyc	RSOL267608:RSP0800-MONOMER.
BRENDA	2.7.7.7. 97066.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_RALSO

Accession

Primary (citable) accession number: Q8XRN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)