Q8XQG4 (NADB2_RALSO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-aspartate oxidase 2
Short name=LASPO 2
EC=1.4.3.16

Alternative name(s):
Quinolinate synthase B 2

Gene names

Name:	nadB2
Ordered Locus Names:	RSp1263
ORF Names:	RS05308

Encoded on

Plasmid megaplasmid Rsp

Organism

Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

267608 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + O2 = iminosuccinate + H2O2.
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC L-aspartate:fumarate oxidoreductase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 536	536	L-aspartate oxidase 2		PRO_0000184395
Regions
	Nucleotide binding	18 – 32	15	FAD Potential
Sites
	Active site	238	1	By similarity
	Active site	257	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XQG4 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: BDF545C8A33E82E7	FASTA	536	57,653
	10 20 30 40 50 60 MTSGQSKHEI RDMKFDVLVV GEGLAALTLL LHLPPSLKIG VISRNKYDEP SSYWAQGGIS 70 80 90 100 110 120 AVFSTDDDHD KHIRDTLLAG DGLCDEAAVR QIVCEGGDVL RWLIDQGVPF TREDGEIHLT 130 140 150 160 170 180 REGGHSERRV AHVDDMTGRG IMRALQAKVA QLPNVIWIRQ YEAVELLSDG QAVSGVIAES 190 200 210 220 230 240 LADGEVTVFS APTVVLAAGG LTGLYQYATN PHASKGEAIA MAWRAGATIE NLEFVQFHPT 250 260 270 280 290 300 AFQIEGRVIS LITEAVRGEG GLLYNVANER FMPGYSSQQE LAPRDVVARA IYSEMQAHGT 310 320 330 340 350 360 SHVWLDITHQ GTAFVEQHFP NLVEITRAHG CDLSQHRVPV SPAAHYTCGG IGADVAGRTN 370 380 390 400 410 420 IEGLYAIGEV ANCGLHGANR LASNSLLECV VMGKACAQSV TDTAGSASRL RLTLPERIET 430 440 450 460 470 480 PFHPNLLADL RAILWNHAGI VRSNTGLEIG LQNMAQLQER HASVLPYGQA LRAQNIFDAA 490 500 510 520 530 HLVLLSAAAR KESRGGHFNK DHADKAEAQT TQIPGVPVNF WAAGENASHA AQLAAA

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL646053 Genomic DNA. Translation: CAD18414.1.
RefSeq	NP_522822.1. NC_003296.1.
3D structure databases
ProteinModelPortal	Q8XQG4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1223575.
GenomeReviews	Gene locus RSp1263 in contig AL646053_GR.
KEGG	rso:RSp1263.
NMPDR	fig|267608.1.peg.4701.
PATRIC	20268175. VBIRalSol70888_4755.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG293998.
OMA	NVANERF.
ProtClustDB	CLSK2754027.
Enzyme and pathway databases
BioCyc	RSOL267608:RSP1263-MONOMER.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR015939. Fum_Rdtase/Succ_DH_flav-like_C. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR005288. NadB. [Graphical view]
Gene3D	G3DSA:1.20.58.100. Fum_Rdtase/Succ_DH_flav-like_C. 1 hit.
KO	K00278.
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
SUPFAM	SSF46977. Succ_DH_flav_C. 1 hit.
TIGRFAMs	TIGR00551. NadB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB2_RALSO

Accession

Primary (citable) accession number: Q8XQG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program