Reviewed, UniProtKB/Swiss-Prot Q8XQB8 (NOSZ_RALSO)

Names and origin

Protein names

Recommended name:
    Nitrous-oxide reductase
    EC=1.7.99.6
Alternative name(s):
    N(2)OR
    N2O reductase

Gene names

Name:	nosZ
Ordered Locus Names:	RSp1368
ORF Names:	RS02090

Encoded on

Plasmid megaplasmid Rsp

Organism

Ralstonia solanacearum (Pseudomonas solanacearum) [Complete proteome] [HAMAP]

Taxonomic identifier

305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Ralstonia

Protein attributes

Sequence length	646 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide By similarity.
Catalytic activity	N(2) + H(2)O + acceptor = N(2)O + reduced acceptor.
Cofactor	Binds 2 calcium ions per subunit By similarity. Binds 6 copper ions per subunit. Each subunit contains 2 copper centers; Cu(A) (binuclear) and Cu(Z) (tetranuclear). Cu(Z) is thought to be the site of nitrous oxide reduction By similarity.
Pathway	Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 4/4.
Subunit structure	Homodimer By similarity.
Subcellular location	PeriplasmBy similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the nosZ family. In the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Calcium Copper Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: InterPro periplasmic space Inferred from electronic annotation. Source: HAMAP
Molecular function	calcium ion binding Inferred from electronic annotation. Source: HAMAP copper ion binding Inferred from electronic annotation. Source: HAMAP cytochrome-c oxidase activity Inferred from electronic annotation. Source: InterPro nitrous-oxide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 52	52	Tat-type signal Potential
	Chain	53 – 646	594	Nitrous-oxide reductase		PRO_0000019832
Regions
	Region	551 – 646	96	COX2-like
Sites
	Metal binding	140	1	Copper Z2 By similarity
	Metal binding	141	1	Copper Z3 By similarity
	Metal binding	189	1	Copper Z2 By similarity
	Metal binding	272	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	275	1	Calcium 2 By similarity
	Metal binding	283	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	289	1	Calcium 2 By similarity
	Metal binding	342	1	Calcium 2 By similarity
	Metal binding	344	1	Copper Z1 By similarity
	Metal binding	400	1	Copper Z1 By similarity
	Metal binding	452	1	Copper Z3 By similarity
	Metal binding	473	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	488	1	Calcium 1 By similarity
	Metal binding	513	1	Copper Z4 By similarity
	Metal binding	592	1	Copper A1 By similarity
	Metal binding	627	1	Copper A1 By similarity
	Metal binding	627	1	Copper A2 By similarity
	Metal binding	629	1	Copper A2; via carbonyl oxygen By similarity
	Metal binding	631	1	Copper A1 By similarity
	Metal binding	631	1	Copper A2 By similarity
	Metal binding	635	1	Copper A2 By similarity
	Metal binding	638	1	Copper A1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XQB8-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 82A70B60D48271E0	FASTA	646	71,307
	10 20 30 40 50 60 MMSKHPHSPS TPQDETPSVP GRRRFMNSAA LAGLATVVAC TDKGAPAGSA AATGVAATAE 70 80 90 100 110 120 EHTVAGLHPK PGQLDTYYGL WSGGHTGDMR VMGMPSGREI HRIPMFVPDA LVGWGITNES 130 140 150 160 170 180 KKVMGTRPDG RLKYTVGDTH HVHASYKDGN YDGRYAWIND KINSRLGRVR LDYFICDKIT 190 200 210 220 230 240 ELPNVQGFHG IFPDKRDPVD PQINYTTRVF CGGEFAIPLP NTAGIDDPAK YRSLFTCVDA 250 260 270 280 290 300 ESMEVRWQVL IDGNCDLVAT SYDGKLAATN QYNTEMGAHY EDMMSAERDA CLFFNVARIE 310 320 330 340 350 360 AAVKAGRFKT IGDSKVPVVD GTHAANQDPK TALTAYVSVP KNPHGVNASP DQKYFICAGK 370 380 390 400 410 420 LSPTATVIEL ARVLDWFDGK LAKIDDAIVA EVELGLGPLH TAFDGRGNAY TTLFLDSQIV 430 440 450 460 470 480 KWNIDAAIRF HKGDKNAKYV VDRLDVHYQP GHLNASQSET VAADGKFLAV GCKFSKDRFL 490 500 510 520 530 540 PVGPLHPENE QFIDISGDKM VLLQDHPIRS EPHDFIIFKR ELLHPKQIYS LDDFPLATKD 550 560 570 580 590 600 PKQSGVVRNG KKVTVRLTSQ APSYSLREFK LKKGDEVTLI LTNLDKVEDL THGFAIPKYD 610 620 630 640 INFIVNPQET ASVTFIADKP GVFWCYCTHF CHALHLEMRS RMIVEA

References

[1]

"Genome sequence of the plant pathogen Ralstonia solanacearum." Salanoubat M., Genin S., Artiguenave F., Gouzy J., Mangenot S., Arlat M., Billault A., Brottier P., Camus J.-C., Cattolico L., Chandler M., Choisne N., Claudel-Renard C., Cunnac S., Demange N., Gaspin C., Lavie M., Moisan A. , Robert C., Saurin W., Schiex T., Siguier P., Thebault P., Whalen M., Wincker P., Levy M., Weissenbach J., Boucher C.A. Nature 415:497-502(2002) [PubMed: 11823852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: GMI1000.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL646053 Genomic DNA. Translation: CAD18519.1.
RefSeq	NP_522927.1.
3D structure databases
HSSP	HSSP built from PDB template 1FWX based on UniProtKB Q51705.
ModBase	Search...
Genome annotation databases
GeneID	1223680.
GenomeReviews	Gene locus RSp1368 in contig AL646053_GR.
KEGG	rso:RSp1368.
NMPDR	fig|267608.1.peg.4806.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8XQB8.
Enzyme and pathway databases
BioCyc	RSOL267608:RSP1368-MON.
Family and domain databases
HAMAP	MF_00716. [Tree]
InterPro	IPR001505. Copper_CuA. IPR002429. COX2_C. IPR008972. Cupredoxin. IPR015943. WD40/YVTN_repeat-like. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 1 hit. G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
Pfam	PF00116. COX2. 1 hit. [Graphical view]
PROSITE	PS00078. COX2. False negative. PS50857. COX2_CUA. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOSZ_RALSO

Accession

Primary (citable) accession number: Q8XQB8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2003
Last sequence update:	March 1, 2002
Last modified:	November 25, 2008
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)