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Protein

Chemotaxis response regulator protein-glutamate methylesterase

Gene

cheB

Organism
Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR.UniRule annotation

Catalytic activityi

Protein L-glutamate O5-methyl ester + H2O = protein L-glutamate + methanol.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei195UniRule annotation1
Active sitei221UniRule annotation1
Active sitei317UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processChemotaxis

Names & Taxonomyi

Protein namesi
Recommended name:
Chemotaxis response regulator protein-glutamate methylesteraseUniRule annotation (EC:3.1.1.61UniRule annotation)
Gene namesi
Name:cheBUniRule annotation
Ordered Locus Names:RSp1403
ORF Names:RS02055
Encoded oniPlasmid megaplasmid Rsp0 Publication
OrganismiRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)
Taxonomic identifieri267608 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia
Proteomesi
  • UP000001436 Componenti: Plasmid megaplasmid Rsp

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001580171 – 381Chemotaxis response regulator protein-glutamate methylesteraseAdd BLAST381

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei594-aspartylphosphateUniRule annotation1

Post-translational modificationi

Phosphorylated by CheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain.UniRule annotation

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliQ8XQ83.
SMRiQ8XQ83.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 125Response regulatoryPROSITE-ProRule annotationAdd BLAST118
Domaini183 – 375CheB-type methylesteraseUniRule annotationAdd BLAST193

Domaini

The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000151423.
KOiK03412.
OMAiMLEMHRA.
OrthoDBiPOG091H045J.

Family and domain databases

CDDicd16432. CheB_Rec. 1 hit.
cd00156. REC. 1 hit.
Gene3Di3.40.50.180. 1 hit.
HAMAPiMF_00099. CheB_methylest. 1 hit.
InterProiView protein in InterPro
IPR035909. CheB_C.
IPR011006. CheY-like_superfamily.
IPR008248. Sig_transdc_resp-reg_CheB.
IPR000673. Sig_transdc_resp-reg_Me-estase.
IPR001789. Sig_transdc_resp-reg_receiver.
PfamiView protein in Pfam
PF01339. CheB_methylest. 1 hit.
PF00072. Response_reg. 1 hit.
PIRSFiPIRSF000876. RR_chemtxs_CheB. 1 hit.
SMARTiView protein in SMART
SM00448. REC. 1 hit.
SUPFAMiSSF52172. SSF52172. 1 hit.
SSF52738. SSF52738. 1 hit.
PROSITEiView protein in PROSITE
PS50122. CHEB. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8XQ83-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEKRKIQVL CIDDSALIRS ILKEIINAQP DMEVVGVAPD PIIARDLIKQ
60 70 80 90 100
TNPDVLTLDV EMPKMDGLDF LEKLMRLRPT PVVMISSLTE RGSEATLRAL
110 120 130 140 150
ELGAVDFVAK PKLGMRDGMN EYADQIADKI RAAARARLRP RTASPAPAPA
160 170 180 190 200
AASPAHAVHT AHAVHARPEH DAAAVPAPAR THFSSTEKLI IVGASTGGTE
210 220 230 240 250
AIKDFLLEMP PDCPGILIVQ HMPAGFTTSF AKRLDGLCRI RVKEAVHGER
260 270 280 290 300
VLPGHAYIAP GDMHLSLGRS GANYVTELDQ NPPVNRHRPA VDVLFRSAAR
310 320 330 340 350
NAGANALGVI LTGMGKDGAA GMLEMRNAGA YNVAQDEASC VVYGMPKEAV
360 370 380
AHGGVHEILP LHQIGPHVLA RLSAHGRVTR V
Length:381
Mass (Da):40,762
Last modified:March 1, 2002 - v1
Checksum:i027C7A7197E4523D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL646053 Genomic DNA. Translation: CAD18554.1.
RefSeqiWP_011004653.1. NC_003296.1.

Genome annotation databases

EnsemblBacteriaiCAD18554; CAD18554; RSp1403.
GeneIDi1223715.
KEGGirso:RSp1403.
PATRICifig|267608.8.peg.4882.

Similar proteinsi

Entry informationi

Entry nameiCHEB_RALSO
AccessioniPrimary (citable) accession number: Q8XQ83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 1, 2002
Last modified: November 22, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome