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Q8XPA2 (GLGB1_CLOPE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB 1

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 1
Alpha-(1->4)-glucan branching enzyme 1
Glycogen branching enzyme 1
Short name=BE 1
Gene names
Name:glgB1
Ordered Locus Names:CPE0063
OrganismClostridium perfringens (strain 13 / Type A) [Complete proteome] [HAMAP]
Taxonomic identifier195102 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length674 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6746741,4-alpha-glucan branching enzyme GlgB 1 HAMAP-Rule MF_00685
PRO_0000188695

Sites

Active site3361Nucleophile By similarity
Active site3891Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8XPA2 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 0178859EB7CC70DB

FASTA67480,323
        10         20         30         40         50         60 
MTLVEVKDDK LEIKPVRLRK EKYKTQLKNK IFNEDDLYLF HEGRNYNAYN FMGAHFTSEN 

        70         80         90        100        110        120 
RKRGVRFTLW APRAKNIFLV GDFSNWETKE ENKLERINET GLWSIFIPRL KEGIKYKYYI 

       130        140        150        160        170        180 
EQEDGKAVLK ADPYGIYSEV RPNTASILCE KTKIRWSDKK WLNKREETNY FESPINIYEL 

       190        200        210        220        230        240 
HLGSWKRKDE DEFLSYDELS IILPKYIKEM GYTHVEFMPL NEHPLDASWG YQVTGYYSIT 

       250        260        270        280        290        300 
SRYGDIKGLK RLINALHKED IGVILDWVPG HFCKDEQGLY MFDGTPTYEY EEKWKADNKG 

       310        320        330        340        350        360 
WGTFNFDLGK PEVKSFLISN AFYFINEFHI DGLRVDAVSN MLYLNYGRNH GEWVPNIYGG 

       370        380        390        400        410        420 
NENLEAIQFI KELNEAIKTY SKGVITIAEE STSWPNVTND TEYGGLGFDF KWNMGWMNDT 

       430        440        450        460        470        480 
LEYNELDPIY RKYHHNKLTF PMMYNHSEKF ILPISHDEVV HGKKSLIDKM QGDYWNKLAN 

       490        500        510        520        530        540 
LRAYMAYMYG HPGKKLMFMG CEFGQFIEWR EYEELEWKLI DKFDMHRKTH NFFKDLNNFY 

       550        560        570        580        590        600 
KNNSELWELD YDQDGFQWID ADNNEQSIYI FIRKSKNIEK YKIFVCNFTP MVYYDFNIGV 

       610        620        630        640        650        660 
PEKGVYREIF NTDKEEYGGS GQVIKGNLFS RKGWCHNQQY TLTIKVPPMA VSVFERIIEE 

       670 
NKTEEKIVKE DKYI 

« Hide

References

[1]"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater."
Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H.
Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 13 / Type A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000016 Genomic DNA. Translation: BAB79769.1.
RefSeqNP_560979.1. NC_003366.1.

3D structure databases

ProteinModelPortalQ8XPA2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING195102.CPE0063.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB79769; BAB79769; BAB79769.
GeneID988296.
KEGGcpe:CPE0063.
PATRIC19493992. VBICloPer59675_0121.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAECHQNNI.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycCPER195102:GJFM-80-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 2 hits.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB1_CLOPE
AccessionPrimary (citable) accession number: Q8XPA2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2002
Last modified: June 11, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries