Reviewed, UniProtKB/Swiss-Prot Q8XPA2 (GLGB1_CLOPE)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme 1
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme 1
      Short name=BE 1
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 1

Gene names

Name:	glgB1
Ordered Locus Names:	CPE0063

Organism

Clostridium perfringens [Complete proteome] [HAMAP]

Taxonomic identifier

1502 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	674 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 674	674	1,4-alpha-glucan-branching enzyme 1 HAMAP MF_00685		PRO_0000188695
Sites
	Active site	231	1	By similarity
	Active site	266	1	By similarity
	Active site	271	1	By similarity
	Active site	334	1	By similarity
	Active site	336	1	By similarity
	Active site	389	1	By similarity
	Active site	456	1	By similarity
	Active site	457	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XPA2-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 0178859EB7CC70DB	FASTA	674	80,323
	10 20 30 40 50 60 MTLVEVKDDK LEIKPVRLRK EKYKTQLKNK IFNEDDLYLF HEGRNYNAYN FMGAHFTSEN 70 80 90 100 110 120 RKRGVRFTLW APRAKNIFLV GDFSNWETKE ENKLERINET GLWSIFIPRL KEGIKYKYYI 130 140 150 160 170 180 EQEDGKAVLK ADPYGIYSEV RPNTASILCE KTKIRWSDKK WLNKREETNY FESPINIYEL 190 200 210 220 230 240 HLGSWKRKDE DEFLSYDELS IILPKYIKEM GYTHVEFMPL NEHPLDASWG YQVTGYYSIT 250 260 270 280 290 300 SRYGDIKGLK RLINALHKED IGVILDWVPG HFCKDEQGLY MFDGTPTYEY EEKWKADNKG 310 320 330 340 350 360 WGTFNFDLGK PEVKSFLISN AFYFINEFHI DGLRVDAVSN MLYLNYGRNH GEWVPNIYGG 370 380 390 400 410 420 NENLEAIQFI KELNEAIKTY SKGVITIAEE STSWPNVTND TEYGGLGFDF KWNMGWMNDT 430 440 450 460 470 480 LEYNELDPIY RKYHHNKLTF PMMYNHSEKF ILPISHDEVV HGKKSLIDKM QGDYWNKLAN 490 500 510 520 530 540 LRAYMAYMYG HPGKKLMFMG CEFGQFIEWR EYEELEWKLI DKFDMHRKTH NFFKDLNNFY 550 560 570 580 590 600 KNNSELWELD YDQDGFQWID ADNNEQSIYI FIRKSKNIEK YKIFVCNFTP MVYYDFNIGV 610 620 630 640 650 660 PEKGVYREIF NTDKEEYGGS GQVIKGNLFS RKGWCHNQQY TLTIKVPPMA VSVFERIIEE 670 NKTEEKIVKE DKYI

References

[1]

"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000016 Genomic DNA. Translation: BAB79769.1.
RefSeq	NP_560979.1.
3D structure databases
HSSP	HSSP built from PDB template 1M7X based on UniProtKB P07762.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	988296.
GenomeReviews	Gene locus CPE0063 in contig BA000016_GR.
KEGG	cpe:CPE0063.
NMPDR	fig|195102.1.peg.126.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8XPA2.
OMA	EHPYDPS.
Enzyme and pathway databases
BioCyc	CPER195102:CPE0063-MON.
BRENDA	2.4.1.18. 2406.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB1_CLOPE

Accession

Primary (citable) accession number: Q8XPA2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)