Reviewed, UniProtKB/Swiss-Prot Q8XNH2 (K6PF1_CLOPE)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase 1
    EC=2.7.1.11
Alternative name(s):
    Phosphofructokinase 1
    Phosphohexokinase 1

Gene names

Name:	pfkA1
Synonyms:	pfk
Ordered Locus Names:	CPE0361

Organism

Clostridium perfringens [Complete proteome] [HAMAP]

Taxonomic identifier

1502 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	6-phosphofructokinase 1 HAMAP MF_00339		PRO_0000111944
Regions
	Nucleotide binding	21 – 25	5	ATP By similarity
	Nucleotide binding	154 – 158	5	ATP By similarity
	Nucleotide binding	171 – 187	17	ATP By similarity
Sites
	Active site	127	1	Proton acceptor By similarity
	Metal binding	185	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	187	1	Magnesium By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	243	1	Substrate By similarity
	Binding site	249	1	Substrate By similarity
	Binding site	252	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XNH2-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 95DE9CAFBA11DF48	FASTA	319	34,066
	10 20 30 40 50 60 MKKIAVLTSG GDAPGMNAAL RAVTRMALHH GLEVMGVQRG YAGLINGELF KMDRKSVSEI 70 80 90 100 110 120 INRGGTILRT ARCLEFKQEE VREKAAQILK AYGVEALVVI GGDGSFMGAK LLSKLGVKTV 130 140 150 160 170 180 GLPGTIDNDL SYTDYTIGFD TALNTVVDAI NKLRDTSTSH ERVSIVEVMG RNCGDLALYA 190 200 210 220 230 240 GIAGGAEAII VPEMPFDKDE LIKTILEGRT NGKTHSIIVV AEGVGGSAEL AKEIESVTGI 250 260 270 280 290 300 ETRATILGHI QRGGSPTADD IVLASRMGAK AVEVLLEGKT SKVIGIKENR IFDMDIDEAL 310 DIERSFDESL YEMANAINK

References

[1]

"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000016 Genomic DNA. Translation: BAB80067.1.
RefSeq	NP_561277.1.
3D structure databases
HSSP	HSSP built from PDB template 6PFK based on UniProtKB P00512.
ModBase	Search...
Genome annotation databases
GeneID	988614.
GenomeReviews	Gene locus CPE0361 in contig BA000016_GR.
KEGG	cpe:CPE0361.
NMPDR	fig|195102.1.peg.424.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8XNH2.
OMA	DTCLNTV.
Enzyme and pathway databases
BioCyc	CPER195102:CPE0361-MON.
BRENDA	2.7.1.11. 2406.
Family and domain databases
HAMAP	MF_00339. [Tree]
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
ProDom	PD000707. Ppfruckinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF1_CLOPE

Accession

Primary (citable) accession number: Q8XNH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)