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Protein

Quinolinate synthase A

Gene

nadA

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.UniRule annotation

Catalytic activityi

Glycerone phosphate + iminosuccinate = pyridine-2,3-dicarboxylate + 2 H2O + phosphate.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster per subunit.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes quinolinate from iminoaspartate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Quinolinate synthase A (nadA)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes quinolinate from iminoaspartate, the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei21 – 211IminoaspartateUniRule annotation
Binding sitei38 – 381Iminoaspartate; via amide nitrogenUniRule annotation
Binding sitei109 – 1091IminoaspartateUniRule annotation
Binding sitei126 – 1261IminoaspartateUniRule annotation
Binding sitei195 – 1951IminoaspartateUniRule annotation
Binding sitei212 – 2121IminoaspartateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Pyridine nucleotide biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciCPER195102:GJFM-431-MONOMER.
UniPathwayiUPA00253; UER00327.

Names & Taxonomyi

Protein namesi
Recommended name:
Quinolinate synthase AUniRule annotation (EC:2.5.1.72UniRule annotation)
Gene namesi
Name:nadAUniRule annotation
Ordered Locus Names:CPE0394
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000818 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301Quinolinate synthase APRO_0000155785Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ8XNE3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the quinolinate synthase A family. Type 2 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000222770.
KOiK03517.
OMAiDKCLGEN.
OrthoDBiEOG6DJZ56.

Family and domain databases

HAMAPiMF_00568. NadA_type2.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8XNE3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIRDEILKL KKEKGAIILA HYYQIPEIQE IADYVGDSYY LSKIAKDCEE
60 70 80 90 100
NIIVFCGVKF MAESAKILSP EKTVILPVME AGCVMADMAT EEGLAKLKEE
110 120 130 140 150
HPNAKVVCYI NSSTEVKALS DVCCTSSNAE NIINNLEEKE IIFLPDRNLG
160 170 180 190 200
SYIQEKTPDK KFILWNGFCI VHEAIQKEEI LRLKSEHEGI LTVAHPECSK
210 220 230 240 250
EIRDISDFIG STSEIINFVN NSSNKKFIII TEEGVLHQLR KNGEEKEFYI
260 270 280 290 300
PYGKMVCRNM KMTTLKDLYE SLLKMENKIE IDEDLRLKAY NSLKNMHKLG

G
Length:301
Mass (Da):34,328
Last modified:March 1, 2002 - v1
Checksum:i67898B2EDFA5B649
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA. Translation: BAB80100.1.
RefSeqiWP_003455349.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB80100; BAB80100; BAB80100.
GeneIDi988647.
KEGGicpe:CPE0394.
PATRICi19494696. VBICloPer59675_0453.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA. Translation: BAB80100.1.
RefSeqiWP_003455349.1. NC_003366.1.

3D structure databases

ProteinModelPortaliQ8XNE3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB80100; BAB80100; BAB80100.
GeneIDi988647.
KEGGicpe:CPE0394.
PATRICi19494696. VBICloPer59675_0453.

Phylogenomic databases

HOGENOMiHOG000222770.
KOiK03517.
OMAiDKCLGEN.
OrthoDBiEOG6DJZ56.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00327.
BioCyciCPER195102:GJFM-431-MONOMER.

Family and domain databases

HAMAPiMF_00568. NadA_type2.
InterProiIPR003473. NadA.
IPR023066. Quinolinate_synth_type2.
[Graphical view]
PfamiPF02445. NadA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00550. nadA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13 / Type A.

Entry informationi

Entry nameiNADA_CLOPE
AccessioniPrimary (citable) accession number: Q8XNE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: March 1, 2002
Last modified: December 9, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.