Reviewed, UniProtKB/Swiss-Prot Q8XNE2 (NADB_CLOPE)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:	nadB
Ordered Locus Names:	CPE0395

Organism

Clostridium perfringens [Complete proteome] [HAMAP]

Taxonomic identifier

1502 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + H(2)O + O(2) = oxaloacetate + NH(3) + H(2)O(2).
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 439	439	L-aspartate oxidase		PRO_0000184383
Regions
	Nucleotide binding	8 – 22	15	FAD Potential
Sites
	Active site	225	1	By similarity
	Active site	246	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XNE2-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: D89A50E494E5F5C7	FASTA	439	49,236
	10 20 30 40 50 60 MKRVADVLIV GSGVAGLYAS LNLREDLEII MVSKKSVNLC NSSLAQGGIA VARGKEDFQS 70 80 90 100 110 120 FIEDTLKAGK YENNIDSVRV LVEESMDNIN KLIDLGANFE KDENGVLFTK EGAHEINRIV 130 140 150 160 170 180 YHKDITGKHV EDILLENVKR RKNIKIIEDC EMVDIYHRGN RCIGALFNKD GQDLSIYAKV 190 200 210 220 230 240 VILATGGIGG LFKKSTNERI ITGDSIGVAI RNNIEIKDLS YIQIHPTAFF SKKSEEKRFL 250 260 270 280 290 300 ISESVRGEGG KLLNCNGERF VDELLPRDIV SKKIYEEMKK TNSNNVFLDV SFMEKSFLQN 310 320 330 340 350 360 RFPNIYNKCL EEGIDISKEP IPVAPAQHYF MGGIKVDLNG KTSMENLYAF GETSCTGVHG 370 380 390 400 410 420 ANRLASNSLL EALVFSRRGA LEINSYIDNL ELIIEERECE DLDKYRLLNR KILIDEICRL 430 RGDIKDELVT CGGECKKSS

References

[1]

"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000016 Genomic DNA. Translation: BAB80101.1.
RefSeq	NP_561311.1.
3D structure databases
HSSP	HSSP built from PDB template 1CHU based on UniProtKB P10902.
ModBase	Search...
Genome annotation databases
GeneID	988648.
GenomeReviews	Gene locus CPE0395 in contig BA000016_GR.
KEGG	cpe:CPE0395.
NMPDR	fig|195102.1.peg.458.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8XNE2.
Enzyme and pathway databases
BioCyc	CPER195102:CPE0395-MON.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NADB_CLOPE

Accession

Primary (citable) accession number: Q8XNE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	March 1, 2002
Last modified:	November 25, 2008
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)