Exo-alpha-sialidase - Clostridium perfringens (strain 13 / Type A)

Preferred order of sections

Names and origin

Protein names

Submitted name:
Exo-alpha-sialidase EMBL BAB80259.1

Gene names

Name:

nanJ EMBL BAB80259.1

Organism

Clostridium perfringens (strain 13 / Type A) [Complete proteome] [HAMAP] EMBL BAB80259.1

Taxonomic identifier

195102 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium ›

Protein attributes

Sequence length	1173 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Contains 1 fibronectin type-III domain. RuleBase RU000718

Ontologies

Keywords
Ligand	Calcium PDB 2V72 Metal-binding PDB 2V72
Technical term	3D-structure PDB 2V72 Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell adhesion Inferred from electronic annotation. Source: InterPro
Molecular_function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Region

79 – 82

4

Galactose binding PDB 2V72

Sites

Metal binding

68

1

Calcium; via carbonyl oxygen PDB 2V72

Metal binding

71

1

Calcium PDB 2V72

Metal binding

73

1

Calcium; via carbonyl oxygen PDB 2V72

Metal binding

76

1

Calcium PDB 2V72

Metal binding

174

1

Calcium; via carbonyl oxygen PDB 2V72

Metal binding

175

1

Calcium PDB 2V72

Binding site

110

1

Galactose PDB 2V72

Sequences

Sequence

Length

Mass (Da)

Tools

Q8XMY5 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 276A1E0A43A788CA
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FASTA

1,173

128,782

        10         20         30         40         50         60 
MKSKKIIATL VASLVISNMG GYLVKANPNV NHKAVIIEDR QAIIETAIPQ SEMTASATSE 

        70         80         90        100        110        120 
EGQDPASSAI DGNINTMWHT KWNGSDALPQ SLSVNLGKAR KVSSIAITPR TSGNNGFITK 

       130        140        150        160        170        180 
YEIHAINNGV ETLVAEGTWE ENNLVKTVTF DSPIDAEEIK ITAIQGVGGF ASIAELNVYE 

       190        200        210        220        230        240 
IKGEVDEIAN YGNLKITKEE ERLNITGDLE KFSSLDEGTI VTRFNMNDTS IQSLIGLSDG 

       250        260        270        280        290        300 
NKANNYFSLY VSGGKVGYEL RRQEGNGDFN VHHSADVTFN KGINTLALKI EKGVGAKIFL 

       310        320        330        340        350        360 
NGSLVKTVSD PNIKFLNAIN LNSGFIGKTD RANGYNEYLF RGNIDFMNIY DKPVSDNYLL 

       370        380        390        400        410        420 
RKTGETKAPS EDSLLPDDVY KTQPVELFYP GYLESRGYRI PALETTKKGT VLASIDVRNN 

       430        440        450        460        470        480 
GDHDAPNNNI DVGIRRKEVN GEWEEGKVIL DYPGKSAAID TSLMSATIEE NGIEKERIFL 

       490        500        510        520        530        540 
IVTHFPEGYG FPNTEGGSGY KEIDGKYYFI LKDAQNNEYT VREDGIVYNS EGNQTDYVMK 

       550        560        570        580        590        600 
NDKTLIQNGE EVGNALLSNS PLKAVGTAHI EMIYSDDDGK TWSEPEDLNP GLKKEWMKFF 

       610        620        630        640        650        660 
GTAPGKGIQI KNGEHKGRLV FPIYYTNQNN FQSSAVIYSD DFGETWKLGE SPIDTASVSS 

       670        680        690        700        710        720 
ETVSSGTQLT ECQVVEMPNG QLKLFMRNTG SYTKIATSFD GGATWHDEVP EDTSLREPYC 

       730        740        750        760        770        780 
QLSVINYSGK INGKDAIIFS NPDASSRVNG SVKVGLINEN GTYDNGEPRY EFDWIYNKTV 

       790        800        810        820        830        840 
KPGSFAYSCL TELPDGNLGL FYEGEGAGRM AYTEFDLNYL KFNASEDSPS ASVQSIEVLD 

       850        860        870        880        890        900 
EDLAYNSGEE VSIKVNFNQL VSIIGDRKIT LDIGGVDVPL NMVNYEGKSS AIFKGTIPEG 

       910        920        930        940        950        960 
INQGNYEIKL KENNTLELNT VYNKVSTFNG LDNTGINVQI GELKTTVGNS TIKVNDEVQV 

       970        980        990       1000       1010       1020 
GSAFEAILGI EGLNGDTEVY SAEYLFEYNV EAFILNEITS FNDSLFVKSK EVEPGKVRIL 

      1030       1040       1050       1060       1070       1080 
VASLGNEIEK DSDLVKVNLT PKISSELEVL GLTTALVGAG DGNTHDLELS SKEVKINEEA 

      1090       1100       1110       1120       1130       1140 
SGEIVVNPVQ NFEIPEINKK NVKLTWNAPI TTEGLEGYVI YKDGKKLSEV PAESTEFVVS 

      1150       1160       1170 
KLNRHTIYNF KVAAKYSNGE LSAKESKTIR TAR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.
[2]	"Carbohydrate recognition by a large sialidase toxin from Clostridium perfringens." Boraston A.B., Ficko-Blean E., Healey M. Biochemistry 46:11352-11360(2007) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 42-180 IN COMPLEX WITH CALCIUM AND GALACTOSE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BA000016 Genomic DNA. Translation: BAB80259.1.

RefSeq

NP_561469.1. NC_003366.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2V72	X-ray	2.25	A	42-180	[»]

ProteinModelPortal

Q8XMY5.

SMR

Q8XMY5. Positions 44-180, 185-367, 378-821.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-46267N.

STRING

195102.CPE0553.

Protein family/group databases

CAZy

CBM32. Carbohydrate-Binding Module Family 32.
CBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

BAB80259; BAB80259; BAB80259.

GeneID

988807.

KEGG

cpe:CPE0553.

PATRIC

19495016. VBICloPer59675_0612.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

HOG000120093.

KO

K01186.

OMA

EVDEIAN.

ProtClustDB

CLSK518658.

Enzyme and pathway databases

BioCyc

CPER195102:GJFM-591-MONOMER.

Family and domain databases

Gene3D

2.120.10.10. 2 hits.
2.60.120.200. 1 hit.
2.60.40.10. 1 hit.

InterPro

IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR003961. Fibronectin_type3.
IPR008979. Galactose-bd-like.
IPR004124. Glyco_hydro_33_N.
IPR013783. Ig-like_fold.
IPR026856. Sialidase_fam.
IPR021984. Sialidase_penC.
IPR011040. Sialidases.
[Graphical view]

PANTHER

PTHR10628. PTHR10628. 1 hit.

Pfam

PF00754. F5_F8_type_C. 1 hit.
PF00041. fn3. 1 hit.
PF02973. Sialidase. 1 hit.
PF12135. Sialidase_penC. 1 hit.
[Graphical view]

SMART

SM00060. FN3. 1 hit.
[Graphical view]

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.
SSF49265. FN_III-like. 1 hit.
SSF49785. Gal_bind_like. 1 hit.
SSF50939. Sialidase. 1 hit.

PROSITE

PS50022. FA58C_3. 1 hit.
PS50853. FN3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8XMY5.

Entry information

Entry name

Q8XMY5_CLOPE

Accession

Primary (citable) accession number: Q8XMY5

Entry history

Integrated into UniProtKB/TrEMBL:	March 1, 2002
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)