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Protein
Submitted name:

Exo-alpha-sialidase

Gene

nanJ

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi68 – 681Calcium; via carbonyl oxygenCombined sources
Metal bindingi71 – 711CalciumCombined sources
Metal bindingi73 – 731Calcium; via carbonyl oxygenCombined sources
Metal bindingi76 – 761CalciumCombined sources
Binding sitei110 – 1101GalactoseCombined sources
Metal bindingi174 – 1741Calcium; via carbonyl oxygenCombined sources
Metal bindingi175 – 1751CalciumCombined sources

GO - Molecular functioni

  1. carbohydrate binding Source: InterPro
  2. exo-alpha-sialidase activity Source: InterPro
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cell adhesion Source: InterPro
Complete GO annotation...

Keywords - Ligandi

CalciumCombined sources, Metal-bindingCombined sources

Enzyme and pathway databases

BioCyciCPER195102:GJFM-591-MONOMER.

Protein family/group databases

CAZyiCBM32. Carbohydrate-Binding Module Family 32.
CBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Submitted name:
Exo-alpha-sialidaseImported
Gene namesi
Name:nanJImported
OrganismiClostridium perfringens (strain 13 / Type A)Imported
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000818: Chromosome

Interactioni

Protein-protein interaction databases

DIPiDIP-46267N.
STRINGi195102.CPE0553.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V72X-ray2.25A42-180[»]
ProteinModelPortaliQ8XMY5.
SMRiQ8XMY5. Positions 44-180, 185-367, 378-821.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8XMY5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni79 – 824Galactose bindingCombined sources

Sequence similaritiesi

Contains 1 fibronectin type-III domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000120093.
KOiK01186.
OMAiEMTASAT.
OrthoDBiEOG61CM2G.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
2.60.120.200. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR008965. Carb-bd_dom.
IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR013320. ConA-like_dom.
IPR003961. FN3_dom.
IPR008979. Galactose-bd-like.
IPR004124. Glyco_hydro_33_N.
IPR013783. Ig-like_fold.
IPR026856. Sialidase_fam.
IPR021984. Sialidase_pen_C.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF00754. F5_F8_type_C. 1 hit.
PF00041. fn3. 1 hit.
PF02973. Sialidase. 1 hit.
PF12135. Sialidase_penC. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49384. SSF49384. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 2 hits.
PROSITEiPS50022. FA58C_3. 1 hit.
PS50853. FN3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8XMY5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSKKIIATL VASLVISNMG GYLVKANPNV NHKAVIIEDR QAIIETAIPQ
60 70 80 90 100
SEMTASATSE EGQDPASSAI DGNINTMWHT KWNGSDALPQ SLSVNLGKAR
110 120 130 140 150
KVSSIAITPR TSGNNGFITK YEIHAINNGV ETLVAEGTWE ENNLVKTVTF
160 170 180 190 200
DSPIDAEEIK ITAIQGVGGF ASIAELNVYE IKGEVDEIAN YGNLKITKEE
210 220 230 240 250
ERLNITGDLE KFSSLDEGTI VTRFNMNDTS IQSLIGLSDG NKANNYFSLY
260 270 280 290 300
VSGGKVGYEL RRQEGNGDFN VHHSADVTFN KGINTLALKI EKGVGAKIFL
310 320 330 340 350
NGSLVKTVSD PNIKFLNAIN LNSGFIGKTD RANGYNEYLF RGNIDFMNIY
360 370 380 390 400
DKPVSDNYLL RKTGETKAPS EDSLLPDDVY KTQPVELFYP GYLESRGYRI
410 420 430 440 450
PALETTKKGT VLASIDVRNN GDHDAPNNNI DVGIRRKEVN GEWEEGKVIL
460 470 480 490 500
DYPGKSAAID TSLMSATIEE NGIEKERIFL IVTHFPEGYG FPNTEGGSGY
510 520 530 540 550
KEIDGKYYFI LKDAQNNEYT VREDGIVYNS EGNQTDYVMK NDKTLIQNGE
560 570 580 590 600
EVGNALLSNS PLKAVGTAHI EMIYSDDDGK TWSEPEDLNP GLKKEWMKFF
610 620 630 640 650
GTAPGKGIQI KNGEHKGRLV FPIYYTNQNN FQSSAVIYSD DFGETWKLGE
660 670 680 690 700
SPIDTASVSS ETVSSGTQLT ECQVVEMPNG QLKLFMRNTG SYTKIATSFD
710 720 730 740 750
GGATWHDEVP EDTSLREPYC QLSVINYSGK INGKDAIIFS NPDASSRVNG
760 770 780 790 800
SVKVGLINEN GTYDNGEPRY EFDWIYNKTV KPGSFAYSCL TELPDGNLGL
810 820 830 840 850
FYEGEGAGRM AYTEFDLNYL KFNASEDSPS ASVQSIEVLD EDLAYNSGEE
860 870 880 890 900
VSIKVNFNQL VSIIGDRKIT LDIGGVDVPL NMVNYEGKSS AIFKGTIPEG
910 920 930 940 950
INQGNYEIKL KENNTLELNT VYNKVSTFNG LDNTGINVQI GELKTTVGNS
960 970 980 990 1000
TIKVNDEVQV GSAFEAILGI EGLNGDTEVY SAEYLFEYNV EAFILNEITS
1010 1020 1030 1040 1050
FNDSLFVKSK EVEPGKVRIL VASLGNEIEK DSDLVKVNLT PKISSELEVL
1060 1070 1080 1090 1100
GLTTALVGAG DGNTHDLELS SKEVKINEEA SGEIVVNPVQ NFEIPEINKK
1110 1120 1130 1140 1150
NVKLTWNAPI TTEGLEGYVI YKDGKKLSEV PAESTEFVVS KLNRHTIYNF
1160 1170
KVAAKYSNGE LSAKESKTIR TAR
Length:1,173
Mass (Da):128,782
Last modified:March 1, 2002 - v1
Checksum:i276A1E0A43A788CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA. Translation: BAB80259.1.
RefSeqiNP_561469.1. NC_003366.1.
WP_011009886.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB80259; BAB80259; BAB80259.
GeneIDi988807.
KEGGicpe:CPE0553.
PATRICi19495016. VBICloPer59675_0612.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA. Translation: BAB80259.1.
RefSeqiNP_561469.1. NC_003366.1.
WP_011009886.1. NC_003366.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2V72X-ray2.25A42-180[»]
ProteinModelPortaliQ8XMY5.
SMRiQ8XMY5. Positions 44-180, 185-367, 378-821.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46267N.
STRINGi195102.CPE0553.

Protein family/group databases

CAZyiCBM32. Carbohydrate-Binding Module Family 32.
CBM40. Carbohydrate-Binding Module Family 40.
GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB80259; BAB80259; BAB80259.
GeneIDi988807.
KEGGicpe:CPE0553.
PATRICi19495016. VBICloPer59675_0612.

Phylogenomic databases

HOGENOMiHOG000120093.
KOiK01186.
OMAiEMTASAT.
OrthoDBiEOG61CM2G.

Enzyme and pathway databases

BioCyciCPER195102:GJFM-591-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ8XMY5.

Family and domain databases

Gene3Di2.120.10.10. 2 hits.
2.60.120.200. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 1 hit.
InterProiIPR008965. Carb-bd_dom.
IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR013320. ConA-like_dom.
IPR003961. FN3_dom.
IPR008979. Galactose-bd-like.
IPR004124. Glyco_hydro_33_N.
IPR013783. Ig-like_fold.
IPR026856. Sialidase_fam.
IPR021984. Sialidase_pen_C.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF00754. F5_F8_type_C. 1 hit.
PF00041. fn3. 1 hit.
PF02973. Sialidase. 1 hit.
PF12135. Sialidase_penC. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49384. SSF49384. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 2 hits.
PROSITEiPS50022. FA58C_3. 1 hit.
PS50853. FN3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13 / Type AImported.
  2. "Carbohydrate recognition by a large sialidase toxin from Clostridium perfringens."
    Boraston A.B., Ficko-Blean E., Healey M.
    Biochemistry 46:11352-11360(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.25 ANGSTROMS) OF 42-180 IN COMPLEX WITH CALCIUM AND GALACTOSE.

Entry informationi

Entry nameiQ8XMY5_CLOPE
AccessioniPrimary (citable) accession number: Q8XMY5
Entry historyi
Integrated into UniProtKB/TrEMBL: March 1, 2002
Last sequence update: March 1, 2002
Last modified: January 7, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.