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Q8XMQ5 (SYW_CLOPE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:CPE0633
OrganismClostridium perfringens (strain 13 / Type A) [Complete proteome] [HAMAP]
Taxonomic identifier195102 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length332 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 332332Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136623

Regions

Motif14 – 229"HIGH" region HAMAP-Rule MF_00140
Motif197 – 2015"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site2001ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8XMQ5 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 5C7BA3B918D58BC1

FASTA33237,158
        10         20         30         40         50         60 
MEDKKKIIFS GIKPSGDLTL GNYLGAIKNW TKLQEEYDCY FCVVDLHAIT VKQLPADLRR 

        70         80         90        100        110        120 
RTLEVLAIYI ASGINPEENT LFIQSHVPAH SEASWLLTCN SYMGELSRMT QYKDKSKKMG 

       130        140        150        160        170        180 
DSIGAGLFNY PVLMAADILL YNADLVPVGK DQMQHLELAR DIGTRFNNSY SKTFTIPEGY 

       190        200        210        220        230        240 
VPKEGAKIMD LQDPSKKMSK SDANPNGFIL IMDEPNVIRK KISRAVTDSI GIVNYTDEQP 

       250        260        270        280        290        300 
GVKNLINILC AIKGYTPDEV VKMYDGKGYA EFKNDVAEAI VEELAPVQEK VKALLGDKKA 

       310        320        330 
LEEIYKKGAE KANYAAMKTL RKMQKKIGLI PR 

« Hide

References

[1]"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater."
Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H.
Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 13 / Type A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000016 Genomic DNA. Translation: BAB80339.1.
RefSeqNP_561549.1. NC_003366.1.

3D structure databases

ProteinModelPortalQ8XMQ5.
SMRQ8XMQ5. Positions 6-330.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING195102.CPE0633.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB80339; BAB80339; BAB80339.
GeneID988891.
KEGGcpe:CPE0633.
PATRIC19495188. VBICloPer59675_0694.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000059940.
KOK01867.
OMAGWGQFKP.
OrthoDBEOG686NJQ.
ProtClustDBPRK00927.

Enzyme and pathway databases

BioCycCPER195102:GJFM-675-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYW_CLOPE
AccessionPrimary (citable) accession number: Q8XMQ5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: March 1, 2002
Last modified: February 19, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries