Q8XMP3 (SYQ_CLOPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamine--tRNA ligase
EC=6.1.1.18

Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS

Gene names

Name:	glnS
Synonyms:	gltX
Ordered Locus Names:	CPE0645

Organism

Clostridium perfringens (strain 13 / Type A) [Complete proteome] [HAMAP]

Taxonomic identifier

195102 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › Clostridium perfringens

Protein attributes

Sequence length	552 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 552	552	Glutamine--tRNA ligase HAMAP-Rule MF_00126		PRO_0000195831
Regions
	Motif	33 – 43	11	"HIGH" region HAMAP-Rule MF_00126
	Motif	266 – 270	5	"KMSKS" region HAMAP-Rule MF_00126
Sites
	Binding site	269	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XMP3 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: A349EA7211F66F1D	FASTA	552	63,933
	10 20 30 40 50 60 MSNAEHSSNF IRNIIIDDLD SKKHDTIITR FPPEPNGYLH IGHAKSIVLN FELGKEFNGR 70 80 90 100 110 120 TNLRFDDTNP TKEDTEYVES IKEDVHWLGY NWSELHFASN YFDEMYKRAL LLIKKGKAYV 130 140 150 160 170 180 CDLTPEEIKE YRGTLTEPGK ESPYRNRSIE ENLDLFERMR KGEFEDGSKV LRAKIDMSSP 190 200 210 220 230 240 NINFRDPIIY RIAHASHHNT GDKWCIYPMY DFAHPLEDAI EGITHSICTL EFADHRPLYD 250 260 270 280 290 300 WFVKECEMES VPRQIEFARL NITNTVMSKR KLKQLVDEGI VDGWDDPRMP TVAGLRRRGY 310 320 330 340 350 360 TPKSIRNFCK AIGVAKADST VDSQMLEHFI REDLQETAPR AMAVINPLKL VITNYPEGES 370 380 390 400 410 420 EILEIENNPK DESAGKRAVT FSREVYIERE DFMENPPKKY FRLFPGNEVR LKGAYFVKCN 430 440 450 460 470 480 EVIKDENGEV TEIHCTYDPE TKSGTGFTGR KVKGTIHWVD ANNCIPAEFR LYEPLILDDC 490 500 510 520 530 540 EENEGKHFLE QINPNSLTIC KGFIEPSAKD AKPQDKYQLF RHGYFNVDPN FTSDDNLVFN 550 RIVSLKSSFK LK

References

[1]

"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000016 Genomic DNA. Translation: BAB80351.1.
RefSeq	NP_561561.1. NC_003366.1.
3D structure databases
ProteinModelPortal	Q8XMP3.
SMR	Q8XMP3. Positions 8-548.
ModBase	Search...
Protein-protein interaction databases
STRING	195102.CPE0645.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB80351; BAB80351; BAB80351.
GeneID	988903.
KEGG	cpe:CPE0645.
PATRIC	19495214. VBICloPer59675_0707.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HOG000259232.
KO	K01886.
OMA	RMPTIAG.
ProtClustDB	PRK05347.
Enzyme and pathway databases
BioCyc	CPER195102:GJFM-687-MONOMER.
Family and domain databases
Gene3D	1.10.1160.10. 1 hit. 2.40.240.10. 2 hits. 3.40.50.620. 2 hits.
HAMAP	MF_00126. Gln_tRNA_synth.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ib. IPR022861. Gln_tRNA_ligase_bac. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10119. PTHR10119. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_CLOPE

Accession

Primary (citable) accession number: Q8XMP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 2, 2002
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program