Reviewed, UniProtKB/Swiss-Prot Q8XM71 (DDLB_CLOPE)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase B
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetaseB
    D-Ala-D-Ala ligase B

Gene names

Name:	ddlB
Ordered Locus Names:	CPE0819

Organism

Clostridium perfringens [Complete proteome] [HAMAP]

Taxonomic identifier

1502 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP MF_00047
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00047
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	D-alanine--D-alanine ligase B HAMAP MF_00047		PRO_0000177808
Regions
	Domain	99 – 294	196	ATP-grasp
	Nucleotide binding	126 – 181	56	ATP By similarity
Sites
	Metal binding	248	1	Magnesium or manganese 1 By similarity
	Metal binding	261	1	Magnesium or manganese 1 By similarity
	Metal binding	261	1	Magnesium or manganese 2 By similarity
	Metal binding	263	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XM71-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 7656F554EA6CE03D	FASTA	302	33,877
	10 20 30 40 50 60 MKVGIIMGGI SSEREVSLAS GESIFNHIDK EKYEVSKIII NNKIDILEKV KGLDFALLAL 70 80 90 100 110 120 HGKFGEDGTV QAFLDIMDIP YSGCGALSSS LCMDKNLSKK VLKAENIRTA KWITVKSIEE 130 140 150 160 170 180 IDYEKIEEIG YPVFVKPNNG GSSVATFKVY KKEDIKNSVM EGLKYDEEVI IESFIKGREI 190 200 210 220 230 240 TCPIFNGELF PILEIKSKAD FYDYKQKYAA NGAEHLPVQL EKSLYDEVKE MALKTFEVLK 250 260 270 280 290 300 CEVYARVDMI ISEEGVPYIL EVNTLPGMTA TSLFPQSAES RGISYSKFID LIIETSLNKK IK

References

[1]

"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000016 Genomic DNA. Translation: BAB80525.1.
RefSeq	NP_561735.1.
3D structure databases
HSSP	HSSP built from PDB template 1EHI based on UniProtKB P71454.
ModBase	Search...
Genome annotation databases
GeneID	989078.
GenomeReviews	Gene locus CPE0819 in contig BA000016_GR.
KEGG	cpe:CPE0819.
NMPDR	fig|195102.1.peg.882.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8XM71.
OMA	Q8XM71. PLSIVPE.
Enzyme and pathway databases
BioCyc	CPER195102:CPE0819-MON.
BRENDA	6.3.2.4. 2406.
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDLB_CLOPE

Accession

Primary (citable) accession number: Q8XM71

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	March 1, 2002
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)