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Q8XL57 (PFKA2_CLOPE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase 2

Short name=ATP-PFK 2
Short name=Phosphofructokinase 2
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase 2
Gene names
Name:pfkA2
Synonyms:pfk
Ordered Locus Names:CPE1185
OrganismClostridium perfringens (strain 13 / Type A) [Complete proteome] [HAMAP]
Taxonomic identifier195102 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length366 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_01976

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_01976

Cofactor

Magnesium By similarity. HAMAP-Rule MF_01976

Enzyme regulation

Subject to allosteric activation by ADP and other diphosphonucleosides, and inhibition by phosphoenolpyruvate By similarity. HAMAP-Rule MF_01976

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_01976

Subunit structure

Homodimer or homotetramer By similarity. HAMAP-Rule MF_01976

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01976.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processfructose 6-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_component6-phosphofructokinase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_function6-phosphofructokinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 366366ATP-dependent 6-phosphofructokinase 2 HAMAP-Rule MF_01976
PRO_0000111945

Regions

Nucleotide binding78 – 792ATP By similarity
Nucleotide binding119 – 1224ATP By similarity
Region142 – 1443Substrate binding By similarity
Region186 – 1883Substrate binding By similarity
Region290 – 2934Substrate binding By similarity

Sites

Active site1441Proton acceptor By similarity
Metal binding1201Magnesium; catalytic By similarity
Binding site151ATP; via amide nitrogen By similarity
Binding site1791Substrate; shared with dimeric partner By similarity
Binding site2391Substrate By similarity
Binding site2841Substrate; shared with dimeric partner By similarity
Site1211Important for substrate specificity; cannot use PPi as phosphoryl donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8XL57 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: C5614C41AE638C31

FASTA36639,514
        10         20         30         40         50         60 
MMQPIKKIAI LTGGGDCPGL NAVIRAVTRT AILKYGYEVI GYKFGYRGLY NNDFVKLDLD 

        70         80         90        100        110        120 
SVSGILHRGG TILHSSNKDN LFDYQVEDEN GKIVKKDVSD VGVENLKKEG VDALVVIGGD 

       130        140        150        160        170        180 
GTLTSARDFS RKGVNVIGVP KTIDNDLLAT DVTFGFNTAT EIATEALDRL HTTAESHHRI 

       190        200        210        220        230        240 
MLLEVMGRNA GWIALESGIA GSADVILLPE IPYDINKIVE KVKEREEAGK QFTIIVVAEG 

       250        260        270        280        290        300 
AKPKDGEVVV SKIVDDSPDP IRLGGIANKL AIDLEGLIKN HEIRSTVLGH IQRGGNTSTY 

       310        320        330        340        350        360 
DRILSTKYGV KAVELINSNL FGNMVALKGN KVSYESLENV IGHTKNVDPE GELVNTAKSI 


GISFAD 

« Hide

References

[1]"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater."
Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H.
Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 13 / Type A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000016 Genomic DNA. Translation: BAB80891.1.
RefSeqNP_562101.1. NC_003366.1.

3D structure databases

ProteinModelPortalQ8XL57.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING195102.CPE1185.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB80891; BAB80891; BAB80891.
GeneID989494.
KEGGcpe:CPE1185.
PATRIC19496320. VBICloPer59675_1257.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248869.
KOK00850.
OMAGLMHEDY.
OrthoDBEOG644ZRM.

Enzyme and pathway databases

BioCycCPER195102:GJFM-1230-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_01976. Phosphofructokinase_III.
InterProIPR012003. ATP_PFK_prok.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02483. PFK_mixed. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFKA2_CLOPE
AccessionPrimary (citable) accession number: Q8XL57
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways