Reviewed, UniProtKB/Swiss-Prot Q8XL57 (K6PF2_CLOPE)

Names and origin

Protein names

Recommended name:
    6-phosphofructokinase 2
    EC=2.7.1.11
Alternative name(s):
    Phosphofructokinase 2
    Phosphohexokinase 2

Gene names

Name:	pfkA2
Synonyms:	pfk
Ordered Locus Names:	CPE1185

Organism

Clostridium perfringens [Complete proteome] [HAMAP]

Taxonomic identifier

1502 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP MF_00339
Subcellular location	Cytoplasm By similarity HAMAP MF_00339.
Sequence similarities	Belongs to the phosphofructokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome
Gene Ontology (GO)
Biological process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	6-phosphofructokinase 2 HAMAP MF_00339		PRO_0000111945
Regions
	Nucleotide binding	25 – 29	5	ATP By similarity
	Nucleotide binding	171 – 175	5	ATP By similarity
	Nucleotide binding	188 – 204	17	ATP By similarity
Sites
	Active site	144	1	Proton acceptor By similarity
	Binding site	179	1	Substrate By similarity
	Binding site	284	1	Substrate By similarity
	Binding site	290	1	Substrate By similarity
	Binding site	293	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XL57-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: C5614C41AE638C31	FASTA	366	39,514
	10 20 30 40 50 60 MMQPIKKIAI LTGGGDCPGL NAVIRAVTRT AILKYGYEVI GYKFGYRGLY NNDFVKLDLD 70 80 90 100 110 120 SVSGILHRGG TILHSSNKDN LFDYQVEDEN GKIVKKDVSD VGVENLKKEG VDALVVIGGD 130 140 150 160 170 180 GTLTSARDFS RKGVNVIGVP KTIDNDLLAT DVTFGFNTAT EIATEALDRL HTTAESHHRI 190 200 210 220 230 240 MLLEVMGRNA GWIALESGIA GSADVILLPE IPYDINKIVE KVKEREEAGK QFTIIVVAEG 250 260 270 280 290 300 AKPKDGEVVV SKIVDDSPDP IRLGGIANKL AIDLEGLIKN HEIRSTVLGH IQRGGNTSTY 310 320 330 340 350 360 DRILSTKYGV KAVELINSNL FGNMVALKGN KVSYESLENV IGHTKNVDPE GELVNTAKSI GISFAD

References

[1]

"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000016 Genomic DNA. Translation: BAB80891.1.
RefSeq	NP_562101.1.
3D structure databases
SMR	Q8XL57. Positions 5-360.
ModBase	Search...
Genome annotation databases
GeneID	989494.
GenomeReviews	Gene locus CPE1185 in contig BA000016_GR.
KEGG	cpe:CPE1185.
NMPDR	fig|195102.1.peg.1248.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG405044.
OMA	ESHHRIM.
PhylomeDB	Q8XL57.
Enzyme and pathway databases
BioCyc	CPER195102:CPE1185-MONOMER.
BRENDA	2.7.1.11. 2406.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase. [Tree]
InterPro	IPR012003. ATP_PFK_prok. IPR012829. PFK. IPR000023. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
TIGRFAMs	TIGR02483. PFK_mixed. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF2_CLOPE

Accession

Primary (citable) accession number: Q8XL57

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)