Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8XL57

- PFKA2_CLOPE

UniProt

Q8XL57 - PFKA2_CLOPE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

ATP-dependent 6-phosphofructokinase 2

Gene

pfkA2

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Subject to allosteric activation by ADP and other diphosphonucleosides, and inhibition by phosphoenolpyruvate.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei15 – 151ATP; via amide nitrogenUniRule annotation
Metal bindingi120 – 1201Magnesium; catalyticUniRule annotation
Sitei121 – 1211Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei144 – 1441Proton acceptorUniRule annotation
Binding sitei179 – 1791Substrate; shared with dimeric partnerUniRule annotation
Binding sitei239 – 2391SubstrateUniRule annotation
Binding sitei284 – 2841Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi78 – 792ATPUniRule annotation
Nucleotide bindingi119 – 1224ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPER195102:GJFM-1230-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase 2UniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFK 2UniRule annotation
Short name:
Phosphofructokinase 2UniRule annotation
Alternative name(s):
Phosphohexokinase 2UniRule annotation
Gene namesi
Name:pfkA2UniRule annotation
Synonyms:pfk
Ordered Locus Names:CPE1185
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000818: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366ATP-dependent 6-phosphofructokinase 2PRO_0000111945Add
BLAST

Interactioni

Subunit structurei

Homodimer or homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi195102.CPE1185.

Structurei

3D structure databases

ProteinModelPortaliQ8XL57.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni142 – 1443Substrate bindingUniRule annotation
Regioni186 – 1883Substrate bindingUniRule annotation
Regioni290 – 2934Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. Mixed-substrate PFK group III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248869.
KOiK00850.
OMAiGLMHEDY.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_01976. Phosphofructokinase_III.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02483. PFK_mixed. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8XL57-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMQPIKKIAI LTGGGDCPGL NAVIRAVTRT AILKYGYEVI GYKFGYRGLY
60 70 80 90 100
NNDFVKLDLD SVSGILHRGG TILHSSNKDN LFDYQVEDEN GKIVKKDVSD
110 120 130 140 150
VGVENLKKEG VDALVVIGGD GTLTSARDFS RKGVNVIGVP KTIDNDLLAT
160 170 180 190 200
DVTFGFNTAT EIATEALDRL HTTAESHHRI MLLEVMGRNA GWIALESGIA
210 220 230 240 250
GSADVILLPE IPYDINKIVE KVKEREEAGK QFTIIVVAEG AKPKDGEVVV
260 270 280 290 300
SKIVDDSPDP IRLGGIANKL AIDLEGLIKN HEIRSTVLGH IQRGGNTSTY
310 320 330 340 350
DRILSTKYGV KAVELINSNL FGNMVALKGN KVSYESLENV IGHTKNVDPE
360
GELVNTAKSI GISFAD
Length:366
Mass (Da):39,514
Last modified:March 1, 2002 - v1
Checksum:iC5614C41AE638C31
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000016 Genomic DNA. Translation: BAB80891.1.
RefSeqiNP_562101.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB80891; BAB80891; BAB80891.
GeneIDi989494.
KEGGicpe:CPE1185.
PATRICi19496320. VBICloPer59675_1257.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000016 Genomic DNA. Translation: BAB80891.1 .
RefSeqi NP_562101.1. NC_003366.1.

3D structure databases

ProteinModelPortali Q8XL57.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 195102.CPE1185.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB80891 ; BAB80891 ; BAB80891 .
GeneIDi 989494.
KEGGi cpe:CPE1185.
PATRICi 19496320. VBICloPer59675_1257.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248869.
KOi K00850.
OMAi GLMHEDY.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci CPER195102:GJFM-1230-MONOMER.

Family and domain databases

HAMAPi MF_01976. Phosphofructokinase_III.
InterProi IPR012003. ATP_PFK_prok-type.
IPR012829. PFK.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02483. PFK_mixed. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13 / Type A.

Entry informationi

Entry nameiPFKA2_CLOPE
AccessioniPrimary (citable) accession number: Q8XL57
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3