Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

O-GlcNAcase NagJ

Gene

nagJ

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds carbohydrates. Capable of hydrolyzing the glycosidic link of O-GlcNAcylated proteins. Can bind and deglycosylate O-glycosylated peptides from mammals. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates (in vitro).By similarity

Catalytic activityi

[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O = [protein]-L-serine + N-acetyl-D-glucosamine.By similarity
[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O = [protein]-L-threonine + N-acetyl-D-glucosamine.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei187Substrate; via carbonyl oxygenBy similarity1
Binding sitei218SubstrateBy similarity1
Binding sitei297SubstrateBy similarity1
Active sitei298Proton donorBy similarityCurated1
Binding sitei335SubstrateBy similarity1
Binding sitei401SubstrateBy similarity1
Binding sitei429SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.52 1503

Protein family/group databases

CAZyiCBM32 Carbohydrate-Binding Module Family 32
GH84 Glycoside Hydrolase Family 84

Names & Taxonomyi

Protein namesi
Recommended name:
O-GlcNAcase NagJ (EC:3.2.1.169By similarity)
Alternative name(s):
Beta-N-acetylglucosaminidase
Beta-N-acetylhexosaminidase
Beta-hexosaminidase
Hexosaminidase B
N-acetyl-beta-D-glucosaminidase
N-acetyl-beta-glucosaminidase
Gene namesi
Name:nagJImported
Ordered Locus Names:CPE1234
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000818 Componenti: Chromosome

Pathology & Biotechi

Chemistry databases

DrugBankiDB04699 gamma-butyrolactone

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000025798631 – 1001O-GlcNAcase NagJAdd BLAST971

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
nagHP268313EBI-15722628,EBI-15722600

Protein-protein interaction databases

DIPiDIP-46266N
IntActiQ8XL08, 3 interactors

Structurei

3D structure databases

ProteinModelPortaliQ8XL08
SMRiQ8XL08
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8XL08

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini916 – 1001Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST86

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni179 – 469Catalytic domainBy similarityAdd BLAST291
Regioni394 – 396Substrate bindingBy similarity3

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili515 – 543Sequence analysisAdd BLAST29
Coiled coili573 – 597Sequence analysisAdd BLAST25

Sequence similaritiesi

Belongs to the glycosyl hydrolase 84 family.Sequence analysis

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

HOGENOMiHOG000052598
KOiK01197
OMAiARMEEAC
OrthoDBiPOG091H0IJT

Family and domain databases

CDDicd00063 FN3, 1 hit
Gene3Di2.60.120.260, 1 hit
2.60.40.10, 1 hit
3.30.379.10, 1 hit
InterProiView protein in InterPro
IPR011496 Beta-N-acetylglucosaminidase
IPR008965 CBM2/CBM3_carb-bd_dom_sf
IPR000421 FA58C
IPR003961 FN3_dom
IPR036116 FN3_sf
IPR008979 Galactose-bd-like_sf
IPR017853 Glycoside_hydrolase_SF
IPR029018 Hex-like_dom2
IPR015882 HEX_bac_N
IPR013783 Ig-like_fold
PfamiView protein in Pfam
PF00754 F5_F8_type_C, 1 hit
PF00041 fn3, 1 hit
PF02838 Glyco_hydro_20b, 1 hit
PF07555 NAGidase, 1 hit
SMARTiView protein in SMART
SM00060 FN3, 1 hit
SUPFAMiSSF49265 SSF49265, 1 hit
SSF49384 SSF49384, 1 hit
SSF49785 SSF49785, 1 hit
SSF51445 SSF51445, 1 hit
SSF55545 SSF55545, 1 hit
PROSITEiView protein in PROSITE
PS50853 FN3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8XL08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRKMLKRLL TSAFACMFIA NGLITTTVRA VGPKTGEENQ VLVPNLNPTP
60 70 80 90 100
ENLEVVGDGF KITSSINLVG EEEADENAVN ALREFLTANN IEINSENDPN
110 120 130 140 150
STTLIIGEVD DDIPELDEAL NGTTAENLKE EGYALVSNDG KIAIEGKDGD
160 170 180 190 200
GTFYGVQTFK QLVKESNIPE VNITDYPTVS ARGIVEGFYG TPWTHKDRLD
210 220 230 240 250
QIKFYGENKL NTYIYAPKDD PYHREKWREP YPENEMQRMQ ELIDASAENK
260 270 280 290 300
VDFVFGISPG IDIRFDGEAG EEDFNHLIAK AESLYDMGVR SFAIYWDDIQ
310 320 330 340 350
DKSAAKHAQV LNRFNEEFVK AKGDVKPLIT VPTEYDTGAM VSNGQPRTYT
360 370 380 390 400
RIFAETVDPS IEVMWTGPGV VTNEIPLSDA QLISGIYNRN MAVWWNYPVT
410 420 430 440 450
DYFKGKLALG PMHGLDKGLN QYVDFFTVNP MEHAELSKIS IHTAADYSWN
460 470 480 490 500
MDNYDYDKAW NRAIDMLYGD LAEDMKVFAN HSTRMDNKTW AKSGREDAPE
510 520 530 540 550
LRAKMDELWN KLSSKEDASA LIEELYGEFA RMEEACNNLK ANLPEVALEE
560 570 580 590 600
CSRQLDELIT LAQGDKASLD MIVAQLNEDT EAYESAKEIA QNKLNTALSS
610 620 630 640 650
FAVISEKVAQ SFIQEALSFD LTLINPRTVK ITASSEETSG ENAPASFASD
660 670 680 690 700
GDMNTFWHSK WSSPAHEGPH HLTLELDNVY EINKVKYAPR QDSKNGRITG
710 720 730 740 750
YKVSVSLDGE NFTEVKTGTL EDNAAIKFIE FDSVDAKYVR LDVTDSVSDQ
760 770 780 790 800
ANGRGKFATA AEVNVHGKLK EAAEVTGSVS LEALEEVQVG ENIEVGVGID
810 820 830 840 850
ELVNAEAFAY DFTLNYDENA FEYVEAISDD GVFVNAKKIE DGKVRVLVSS
860 870 880 890 900
LTGEPLPAKE VLAKVVLRAE AKTEGSNLSV TNSSVGDGEG LVHEIAGTEK
910 920 930 940 950
TVNIIEGTSP EIVVNPVRDF KASEINKKNV TVTWTEPETT EGLEGYILYK
960 970 980 990 1000
DGKKVAEIGK DETSYTFKKL NRHTIYNFKI AAKYSNGEVS SKESLTLRTA

R
Length:1,001
Mass (Da):111,109
Last modified:March 1, 2002 - v1
Checksum:iE0C0A1575073946E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA Translation: BAB80940.1
RefSeqiWP_003467250.1, NC_003366.1

Genome annotation databases

EnsemblBacteriaiBAB80940; BAB80940; BAB80940
KEGGicpe:CPE1234

Similar proteinsi

Entry informationi

Entry nameiOGA_CLOPE
AccessioniPrimary (citable) accession number: Q8XL08
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: March 1, 2002
Last modified: March 28, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health