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Protein

O-GlcNAcase NagJ

Gene

nagJ

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Binds carbohydrates. Capable of hydrolyzing the glycosidic link of O-GlcNAcylated proteins. Can bind and deglycosylate O-glycosylated peptides from mammals. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates (in vitro).By similarity

Catalytic activityi

[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O = [protein]-L-serine + N-acetyl-D-glucosamine.By similarity
[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O = [protein]-L-threonine + N-acetyl-D-glucosamine.By similarity
Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei187Substrate; via carbonyl oxygenBy similarity1
Binding sitei218SubstrateBy similarity1
Binding sitei297SubstrateBy similarity1
Active sitei298Proton donorBy similarityCurated1
Binding sitei335SubstrateBy similarity1
Binding sitei401SubstrateBy similarity1
Binding sitei429SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.52. 1503.

Protein family/group databases

CAZyiCBM32. Carbohydrate-Binding Module Family 32.
GH84. Glycoside Hydrolase Family 84.

Names & Taxonomyi

Protein namesi
Recommended name:
O-GlcNAcase NagJ (EC:3.2.1.169By similarity)
Alternative name(s):
Beta-N-acetylglucosaminidase (EC:3.2.1.52By similarity)
Beta-N-acetylhexosaminidase
Beta-hexosaminidase
Hexosaminidase B
N-acetyl-beta-D-glucosaminidase
N-acetyl-beta-glucosaminidase
Gene namesi
Name:nagJImported
Ordered Locus Names:CPE1234
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000818 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
ChainiPRO_000025798631 – 1001O-GlcNAcase NagJAdd BLAST971

Interactioni

Protein-protein interaction databases

DIPiDIP-46266N.

Structurei

3D structure databases

ProteinModelPortaliQ8XL08.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8XL08.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini916 – 1001Fibronectin type-IIIPROSITE-ProRule annotationAdd BLAST86

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni179 – 469Catalytic domainBy similarityAdd BLAST291
Regioni394 – 396Substrate bindingBy similarity3

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili515 – 543Sequence analysisAdd BLAST29
Coiled coili573 – 597Sequence analysisAdd BLAST25

Sequence similaritiesi

Belongs to the glycosyl hydrolase 84 family.Sequence analysis
Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal

Phylogenomic databases

HOGENOMiHOG000052598.
KOiK01197.
OMAiARMEEAC.
OrthoDBiPOG091H0IJT.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.120.260. 1 hit.
2.60.40.10. 1 hit.
2.60.40.680. 1 hit.
3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProiIPR011496. Beta-N-acetylglucosaminidase.
IPR008965. Carb-bd_dom.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR002102. Cohesin_dom.
IPR000421. FA58C.
IPR003961. FN3_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR015882. HEX_bac_N.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF00754. F5_F8_type_C. 1 hit.
PF00041. fn3. 1 hit.
PF02838. Glyco_hydro_20b. 1 hit.
PF07555. NAGidase. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49384. SSF49384. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8XL08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRKMLKRLL TSAFACMFIA NGLITTTVRA VGPKTGEENQ VLVPNLNPTP
60 70 80 90 100
ENLEVVGDGF KITSSINLVG EEEADENAVN ALREFLTANN IEINSENDPN
110 120 130 140 150
STTLIIGEVD DDIPELDEAL NGTTAENLKE EGYALVSNDG KIAIEGKDGD
160 170 180 190 200
GTFYGVQTFK QLVKESNIPE VNITDYPTVS ARGIVEGFYG TPWTHKDRLD
210 220 230 240 250
QIKFYGENKL NTYIYAPKDD PYHREKWREP YPENEMQRMQ ELIDASAENK
260 270 280 290 300
VDFVFGISPG IDIRFDGEAG EEDFNHLIAK AESLYDMGVR SFAIYWDDIQ
310 320 330 340 350
DKSAAKHAQV LNRFNEEFVK AKGDVKPLIT VPTEYDTGAM VSNGQPRTYT
360 370 380 390 400
RIFAETVDPS IEVMWTGPGV VTNEIPLSDA QLISGIYNRN MAVWWNYPVT
410 420 430 440 450
DYFKGKLALG PMHGLDKGLN QYVDFFTVNP MEHAELSKIS IHTAADYSWN
460 470 480 490 500
MDNYDYDKAW NRAIDMLYGD LAEDMKVFAN HSTRMDNKTW AKSGREDAPE
510 520 530 540 550
LRAKMDELWN KLSSKEDASA LIEELYGEFA RMEEACNNLK ANLPEVALEE
560 570 580 590 600
CSRQLDELIT LAQGDKASLD MIVAQLNEDT EAYESAKEIA QNKLNTALSS
610 620 630 640 650
FAVISEKVAQ SFIQEALSFD LTLINPRTVK ITASSEETSG ENAPASFASD
660 670 680 690 700
GDMNTFWHSK WSSPAHEGPH HLTLELDNVY EINKVKYAPR QDSKNGRITG
710 720 730 740 750
YKVSVSLDGE NFTEVKTGTL EDNAAIKFIE FDSVDAKYVR LDVTDSVSDQ
760 770 780 790 800
ANGRGKFATA AEVNVHGKLK EAAEVTGSVS LEALEEVQVG ENIEVGVGID
810 820 830 840 850
ELVNAEAFAY DFTLNYDENA FEYVEAISDD GVFVNAKKIE DGKVRVLVSS
860 870 880 890 900
LTGEPLPAKE VLAKVVLRAE AKTEGSNLSV TNSSVGDGEG LVHEIAGTEK
910 920 930 940 950
TVNIIEGTSP EIVVNPVRDF KASEINKKNV TVTWTEPETT EGLEGYILYK
960 970 980 990 1000
DGKKVAEIGK DETSYTFKKL NRHTIYNFKI AAKYSNGEVS SKESLTLRTA

R
Length:1,001
Mass (Da):111,109
Last modified:March 1, 2002 - v1
Checksum:iE0C0A1575073946E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA. Translation: BAB80940.1.
RefSeqiWP_003467250.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB80940; BAB80940; BAB80940.
KEGGicpe:CPE1234.
PATRICi19496418. VBICloPer59675_1306.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA. Translation: BAB80940.1.
RefSeqiWP_003467250.1. NC_003366.1.

3D structure databases

ProteinModelPortaliQ8XL08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46266N.

Protein family/group databases

CAZyiCBM32. Carbohydrate-Binding Module Family 32.
GH84. Glycoside Hydrolase Family 84.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB80940; BAB80940; BAB80940.
KEGGicpe:CPE1234.
PATRICi19496418. VBICloPer59675_1306.

Phylogenomic databases

HOGENOMiHOG000052598.
KOiK01197.
OMAiARMEEAC.
OrthoDBiPOG091H0IJT.

Enzyme and pathway databases

BRENDAi3.2.1.52. 1503.

Miscellaneous databases

EvolutionaryTraceiQ8XL08.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.120.260. 1 hit.
2.60.40.10. 1 hit.
2.60.40.680. 1 hit.
3.20.20.80. 1 hit.
3.30.379.10. 1 hit.
InterProiIPR011496. Beta-N-acetylglucosaminidase.
IPR008965. Carb-bd_dom.
IPR029018. Chitobiase/Hex_dom_2-like.
IPR002102. Cohesin_dom.
IPR000421. FA58C.
IPR003961. FN3_dom.
IPR008979. Galactose-bd-like.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR015882. HEX_bac_N.
IPR013783. Ig-like_fold.
[Graphical view]
PfamiPF00754. F5_F8_type_C. 1 hit.
PF00041. fn3. 1 hit.
PF02838. Glyco_hydro_20b. 1 hit.
PF07555. NAGidase. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49384. SSF49384. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF55545. SSF55545. 1 hit.
PROSITEiPS50853. FN3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOGA_CLOPE
AccessioniPrimary (citable) accession number: Q8XL08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.