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Q8XKS9 (DDLA_CLOPE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase A

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase A
D-alanylalanine synthetase A
Gene names
Name:ddlA
Ordered Locus Names:CPE1315
OrganismClostridium perfringens (strain 13 / Type A) [Complete proteome] [HAMAP]
Taxonomic identifier195102 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length301 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 301301D-alanine--D-alanine ligase A HAMAP-Rule MF_00047
PRO_0000177807

Regions

Domain99 – 293195ATP-grasp
Nucleotide binding126 – 18156ATP By similarity

Sites

Metal binding2481Magnesium or manganese 1 By similarity
Metal binding2601Magnesium or manganese 1 By similarity
Metal binding2601Magnesium or manganese 2 By similarity
Metal binding2621Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8XKS9 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: A3CA41C8E5C080AD

FASTA30133,182
        10         20         30         40         50         60 
MKVGVIMGGI SSEREISIQS GNSVVHALDK DKYEAIPIVL NEKEDLIEKV KGIDFALLAL 

        70         80         90        100        110        120 
HGKFGEDGTV QSVLKTLGIP FSGCGPLSSA ICMDKDMTKR ILAFGNVRTA RWEMVSSVDE 

       130        140        150        160        170        180 
IDYEKIENLG YPVFVKPNNG GSSVATTLVE SKEAVKDAVL EALKYDTEVM IEEYIKGDEI 

       190        200        210        220        230        240 
TCPIIDGKML PVLAIKPKGK FFDIASKYGD GGADEFIVEL NEDLHKEVEK MALETYKLLK 

       250        260        270        280        290        300 
CDVYARVDML VKDNIPYVLE VNTLPGMTKN SLFPKSAAGI NMSFEELLDT IIEKSLKVNR 


E 

« Hide

References

[1]"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater."
Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H.
Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 13 / Type A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000016 Genomic DNA. Translation: BAB81021.1.
RefSeqNP_562231.1. NC_003366.1.

3D structure databases

ProteinModelPortalQ8XKS9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING195102.CPE1315.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB81021; BAB81021; BAB81021.
GeneID989624.
KEGGcpe:CPE1315.
PATRIC19496576. VBICloPer59675_1385.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMATARWEMV.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycCPER195102:GJFM-1360-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDLA_CLOPE
AccessionPrimary (citable) accession number: Q8XKS9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2002
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways