Q8XK60 (BIOD_CLOPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	CPE1543

Organism

Clostridium perfringens [Complete proteome] [HAMAP]

Taxonomic identifier

1502 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000187959
Regions
	Nucleotide binding	116 – 119	4	ATP By similarity
	Nucleotide binding	179 – 180	2	ATP By similarity
	Nucleotide binding	208 – 210	3	ATP By similarity
Sites
	Metal binding	13	1	Magnesium 1 By similarity
	Metal binding	17	1	Magnesium 2 By similarity
	Metal binding	55	1	Magnesium 2 By similarity
	Metal binding	116	1	Magnesium 2 By similarity
	Binding site	42	1	Substrate By similarity
	Binding site	55	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XK60 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 336974B1360E727D	FASTA	228	25,668
	10 20 30 40 50 60 MEKGVYIIGT NTDIGKTFIS GLILKKLREE GRNAGYYKAV LSGAIKEKNG LIPLDCEEVM 70 80 90 100 110 120 KISGLKESYE NMVSYILENP YSPHLASEVE EVSISMEKIK KDYKSVRDKY DFILCEGSGG 130 140 150 160 170 180 IVCPISFSEK KLMLEDIIKE FNLQIILVSN SGLGTINYTV LTVSYLRNLG LKVKGIILNK 190 200 210 220 FNKSDIIHRD NKKIIKELTG VNNISTVPKI EDIEKYDLNE LNEVLYGI

References

[1]

"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000016 Genomic DNA. Translation: BAB81249.1.
RefSeq	NP_562459.1. NC_003366.1.
3D structure databases
ProteinModelPortal	Q8XK60.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	989853.
GenomeReviews	Gene locus CPE1543 in contig BA000016_GR.
KEGG	cpe:CPE1543.
NMPDR	fig|195102.1.peg.1606.
PATRIC	19497033. VBICloPer59675_1612.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG650065.
OMA	LNNYNHE.
PhylomeDB	Q8XK60.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	CPER195102:CPE1543-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_CLOPE

Accession

Primary (citable) accession number: Q8XK60

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program