Reviewed, UniProtKB/Swiss-Prot Q8XK60 (BIOD_CLOPE)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	CPE1543

Organism

Clostridium perfringens [Complete proteome] [HAMAP]

Taxonomic identifier

1502 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP cobalamin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: InterPro dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	Dethiobiotin synthetase HAMAP MF_00336		PRO_0000187959
Regions
	Nucleotide binding	9 – 17	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XK60-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 336974B1360E727D	FASTA	228	25,668
	10 20 30 40 50 60 MEKGVYIIGT NTDIGKTFIS GLILKKLREE GRNAGYYKAV LSGAIKEKNG LIPLDCEEVM 70 80 90 100 110 120 KISGLKESYE NMVSYILENP YSPHLASEVE EVSISMEKIK KDYKSVRDKY DFILCEGSGG 130 140 150 160 170 180 IVCPISFSEK KLMLEDIIKE FNLQIILVSN SGLGTINYTV LTVSYLRNLG LKVKGIILNK 190 200 210 220 FNKSDIIHRD NKKIIKELTG VNNISTVPKI EDIEKYDLNE LNEVLYGI

References

[1]

"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000016 Genomic DNA. Translation: BAB81249.1.
RefSeq	NP_562459.1.
3D structure databases
SMR	Q8XK60. Positions 3-213.
ModBase	Search...
Genome annotation databases
GeneID	989853.
GenomeReviews	Gene locus CPE1543 in contig BA000016_GR.
KEGG	cpe:CPE1543.
NMPDR	fig|195102.1.peg.1606.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG650065.
OMA	LNNYNHE.
PhylomeDB	Q8XK60.
Enzyme and pathway databases
BioCyc	CPER195102:CPE1543-MONOMER.
BRENDA	6.3.3.3. 2406.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_CLOPE

Accession

Primary (citable) accession number: Q8XK60

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	March 1, 2002
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)