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Q8XK30

- GSHAB_CLOPE

UniProt

Q8XK30 - GSHAB_CLOPE

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Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi728 – 7281Magnesium or manganese 1UniRule annotation
Metal bindingi747 – 7471Magnesium or manganese 1UniRule annotation
Metal bindingi747 – 7471Magnesium or manganese 2UniRule annotation
Metal bindingi749 – 7491Magnesium or manganese 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi548 – 60659ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
  3. glutathione synthase activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPER195102:GJFM-1619-MONOMER.
UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshABUniRule annotation
Alternative name(s):
Gamma-GCS-GSUniRule annotation
Short name:
GCS-GSUniRule annotation
Including the following 2 domains:
Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-ECSUniRule annotation
Short name:
GCSUniRule annotation
Gamma-glutamylcysteine synthetaseUniRule annotation
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshABUniRule annotation
Synonyms:gshFUniRule annotation
Ordered Locus Names:CPE1573
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000818: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 778778Glutathione biosynthesis bifunctional protein GshABPRO_0000192549Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi195102.CPE1573.

Structurei

3D structure databases

ProteinModelPortaliQ8XK30.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini521 – 777257ATP-graspUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 354354Glutamate--cysteine ligaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000156471.
KOiK01919.
OMAiHVEYVKN.
OrthoDBiEOG6BKJ7H.

Family and domain databases

Gene3Di3.30.470.20. 2 hits.
HAMAPiMF_00782. Glut_biosynth.
InterProiIPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8XK30-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVNLDKGLLK IIKDESLEDY FIKANFGLEK ENVRVTESGN LALTPHPKAF
60 70 80 90 100
GDREKNAYIK TDFSESQLEM VTPVCNTLEE VYSFICNLNK VVSLEIMKNG
110 120 130 140 150
EFLWPQSNPP ILPREEEIPI AKLSNREDEL YRENLSYKYG KKKQVISGIH
160 170 180 190 200
YNFSFKEEFI KLLYKELKVE KDFREFKDDI YLRMARNFQK YHWLLIYLTG
210 220 230 240 250
ASPVFHESYI EEIKEEGEKL GEDSYYIKDD TSLRNSSYGY KNKKDYYVSY
260 270 280 290 300
NSIEEYASDI KNLVKDKEIQ SIKEYYNPIR LKSLGSEDML ESLLHKGIDY
310 320 330 340 350
LEVRLLDLDP LSIQGVSKET LYLLHLFMIY TLLKENKEIT YKDQEEFFKN
360 370 380 390 400
HDMVALKGRN EEAVIYENGV PVLLKDKGRE ILSEMDEIVE ILFSNNEEFK
410 420 430 440 450
NVIKRALEKI NNPHDTISEK LIKDIKEEGY INFHMRLAKE YLNNFKNKEF
460 470 480 490 500
NLVGYEDLEL STQILILDAI KRGIEFNIMD RLENFISLSD GEKVEYVKQA
510 520 530 540 550
TKTSKDSYIT ALIMENKLVT KDILRENNIR VPKGKDYDNI DEAKKDFRLF
560 570 580 590 600
KDEKIVIKPK STNFGLGISI FPGEYSREDY DKAVEIAFRE DSSILIEEFM
610 620 630 640 650
TGKEYRFLVI GEEVVGILHR EPANVIGNGE STIEELVSEK NKDPLRGKGY
660 670 680 690 700
KTPLEKIKLG EIEEMFLKNQ GLSFKSIPKN GEKIYLRENS NISTGGDSID
710 720 730 740 750
FTDKIHPSYK EVALKSAKAV KALICGVDMV IDNIEEEAKE KNHGIIELNF
760 770
NPAIHIHCFP YKGENRKAGE KILDLLFN
Length:778
Mass (Da):90,463
Last modified:March 1, 2002 - v1
Checksum:i2008FF46AF06423E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA. Translation: BAB81279.1.
RefSeqiNP_562489.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB81279; BAB81279; BAB81279.
GeneIDi989883.
KEGGicpe:CPE1573.
PATRICi19497095. VBICloPer59675_1643.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA. Translation: BAB81279.1 .
RefSeqi NP_562489.1. NC_003366.1.

3D structure databases

ProteinModelPortali Q8XK30.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 195102.CPE1573.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB81279 ; BAB81279 ; BAB81279 .
GeneIDi 989883.
KEGGi cpe:CPE1573.
PATRICi 19497095. VBICloPer59675_1643.

Phylogenomic databases

eggNOGi COG1181.
HOGENOMi HOG000156471.
KOi K01919.
OMAi HVEYVKN.
OrthoDBi EOG6BKJ7H.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00209 .
UPA00142 ; UER00210 .
BioCyci CPER195102:GJFM-1619-MONOMER.

Family and domain databases

Gene3Di 3.30.470.20. 2 hits.
HAMAPi MF_00782. Glut_biosynth.
InterProi IPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view ]
Pfami PF04262. Glu_cys_ligase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13 / Type A.

Entry informationi

Entry nameiGSHAB_CLOPE
AccessioniPrimary (citable) accession number: Q8XK30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: March 1, 2002
Last modified: November 26, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3