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Q8XK30

- GSHAB_CLOPE

UniProt

Q8XK30 - GSHAB_CLOPE

Protein

Glutathione biosynthesis bifunctional protein GshAB

Gene

gshAB

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine.UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
    ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi728 – 7281Magnesium or manganese 1UniRule annotation
    Metal bindingi747 – 7471Magnesium or manganese 1UniRule annotation
    Metal bindingi747 – 7471Magnesium or manganese 2UniRule annotation
    Metal bindingi749 – 7491Magnesium or manganese 2UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi548 – 60659ATPUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
    3. glutathione synthase activity Source: UniProtKB-HAMAP
    4. magnesium ion binding Source: UniProtKB-HAMAP
    5. manganese ion binding Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciCPER195102:GJFM-1619-MONOMER.
    UniPathwayiUPA00142; UER00209.
    UPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione biosynthesis bifunctional protein GshABUniRule annotation
    Alternative name(s):
    Gamma-GCS-GSUniRule annotation
    Short name:
    GCS-GSUniRule annotation
    Including the following 2 domains:
    Glutamate--cysteine ligaseUniRule annotation (EC:6.3.2.2UniRule annotation)
    Alternative name(s):
    Gamma-ECSUniRule annotation
    Short name:
    GCSUniRule annotation
    Gamma-glutamylcysteine synthetaseUniRule annotation
    Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
    Alternative name(s):
    GSH synthetaseUniRule annotation
    Short name:
    GSUniRule annotation
    Short name:
    GSH-SUniRule annotation
    Short name:
    GSHaseUniRule annotation
    Glutathione synthaseUniRule annotation
    Gene namesi
    Name:gshABUniRule annotation
    Synonyms:gshFUniRule annotation
    Ordered Locus Names:CPE1573
    OrganismiClostridium perfringens (strain 13 / Type A)
    Taxonomic identifieri195102 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000000818: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 778778Glutathione biosynthesis bifunctional protein GshABPRO_0000192549Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi195102.CPE1573.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8XK30.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini521 – 777257ATP-graspUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 354354Glutamate--cysteine ligaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.UniRule annotation
    Contains 1 ATP-grasp domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1181.
    HOGENOMiHOG000156471.
    KOiK01919.
    OMAiHVEYVKN.
    OrthoDBiEOG6BKJ7H.

    Family and domain databases

    Gene3Di3.30.470.20. 2 hits.
    HAMAPiMF_00782. Glut_biosynth.
    InterProiIPR011761. ATP-grasp.
    IPR013816. ATP_grasp_subdomain_2.
    IPR007370. Glu_cys_ligase.
    IPR006335. Glut_biosynth.
    [Graphical view]
    PfamiPF04262. Glu_cys_ligase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8XK30-1 [UniParc]FASTAAdd to Basket

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    MVNLDKGLLK IIKDESLEDY FIKANFGLEK ENVRVTESGN LALTPHPKAF    50
    GDREKNAYIK TDFSESQLEM VTPVCNTLEE VYSFICNLNK VVSLEIMKNG 100
    EFLWPQSNPP ILPREEEIPI AKLSNREDEL YRENLSYKYG KKKQVISGIH 150
    YNFSFKEEFI KLLYKELKVE KDFREFKDDI YLRMARNFQK YHWLLIYLTG 200
    ASPVFHESYI EEIKEEGEKL GEDSYYIKDD TSLRNSSYGY KNKKDYYVSY 250
    NSIEEYASDI KNLVKDKEIQ SIKEYYNPIR LKSLGSEDML ESLLHKGIDY 300
    LEVRLLDLDP LSIQGVSKET LYLLHLFMIY TLLKENKEIT YKDQEEFFKN 350
    HDMVALKGRN EEAVIYENGV PVLLKDKGRE ILSEMDEIVE ILFSNNEEFK 400
    NVIKRALEKI NNPHDTISEK LIKDIKEEGY INFHMRLAKE YLNNFKNKEF 450
    NLVGYEDLEL STQILILDAI KRGIEFNIMD RLENFISLSD GEKVEYVKQA 500
    TKTSKDSYIT ALIMENKLVT KDILRENNIR VPKGKDYDNI DEAKKDFRLF 550
    KDEKIVIKPK STNFGLGISI FPGEYSREDY DKAVEIAFRE DSSILIEEFM 600
    TGKEYRFLVI GEEVVGILHR EPANVIGNGE STIEELVSEK NKDPLRGKGY 650
    KTPLEKIKLG EIEEMFLKNQ GLSFKSIPKN GEKIYLRENS NISTGGDSID 700
    FTDKIHPSYK EVALKSAKAV KALICGVDMV IDNIEEEAKE KNHGIIELNF 750
    NPAIHIHCFP YKGENRKAGE KILDLLFN 778
    Length:778
    Mass (Da):90,463
    Last modified:March 1, 2002 - v1
    Checksum:i2008FF46AF06423E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000016 Genomic DNA. Translation: BAB81279.1.
    RefSeqiNP_562489.1. NC_003366.1.
    WP_011010516.1. NC_003366.1.

    Genome annotation databases

    EnsemblBacteriaiBAB81279; BAB81279; BAB81279.
    GeneIDi989883.
    KEGGicpe:CPE1573.
    PATRICi19497095. VBICloPer59675_1643.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000016 Genomic DNA. Translation: BAB81279.1 .
    RefSeqi NP_562489.1. NC_003366.1.
    WP_011010516.1. NC_003366.1.

    3D structure databases

    ProteinModelPortali Q8XK30.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 195102.CPE1573.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB81279 ; BAB81279 ; BAB81279 .
    GeneIDi 989883.
    KEGGi cpe:CPE1573.
    PATRICi 19497095. VBICloPer59675_1643.

    Phylogenomic databases

    eggNOGi COG1181.
    HOGENOMi HOG000156471.
    KOi K01919.
    OMAi HVEYVKN.
    OrthoDBi EOG6BKJ7H.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00209 .
    UPA00142 ; UER00210 .
    BioCyci CPER195102:GJFM-1619-MONOMER.

    Family and domain databases

    Gene3Di 3.30.470.20. 2 hits.
    HAMAPi MF_00782. Glut_biosynth.
    InterProi IPR011761. ATP-grasp.
    IPR013816. ATP_grasp_subdomain_2.
    IPR007370. Glu_cys_ligase.
    IPR006335. Glut_biosynth.
    [Graphical view ]
    Pfami PF04262. Glu_cys_ligase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01435. glu_cys_lig_rel. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 13 / Type A.

    Entry informationi

    Entry nameiGSHAB_CLOPE
    AccessioniPrimary (citable) accession number: Q8XK30
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 30, 2002
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3