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Q8XK30

- GSHAB_CLOPE

UniProt

Q8XK30 - GSHAB_CLOPE

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Protein
Glutathione biosynthesis bifunctional protein GshAB
Gene
gshAB, gshF, CPE1573
Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Synthesizes glutathione from L-glutamate and L-cysteine via gamma-L-glutamyl-L-cysteine By similarity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation
ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi728 – 7281Magnesium or manganese 1 By similarity
Metal bindingi747 – 7471Magnesium or manganese 1 By similarity
Metal bindingi747 – 7471Magnesium or manganese 2 By similarity
Metal bindingi749 – 7491Magnesium or manganese 2 By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi548 – 60659ATP By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP
  3. glutathione synthase activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP
  5. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCPER195102:GJFM-1619-MONOMER.
UniPathwayiUPA00142; UER00209.
UPA00142; UER00210.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione biosynthesis bifunctional protein GshAB
Alternative name(s):
Gamma-GCS-GS
Short name:
GCS-GS
Including the following 2 domains:
Glutamate--cysteine ligase (EC:6.3.2.2)
Alternative name(s):
Gamma-ECS
Short name:
GCS
Gamma-glutamylcysteine synthetase
Glutathione synthetase (EC:6.3.2.3)
Alternative name(s):
GSH synthetase
Short name:
GS
Short name:
GSH-S
Short name:
GSHase
Glutathione synthase
Gene namesi
Name:gshAB
Synonyms:gshF
Ordered Locus Names:CPE1573
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000818: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 778778Glutathione biosynthesis bifunctional protein GshABUniRule annotation
PRO_0000192549Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi195102.CPE1573.

Structurei

3D structure databases

ProteinModelPortaliQ8XK30.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini521 – 777257ATP-grasp
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 354354Glutamate--cysteine ligaseUniRule annotation
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glutamate--cysteine ligase type 1 family. Type 2 subfamily.
Contains 1 ATP-grasp domain.

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000156471.
KOiK01919.
OMAiHVEYVKN.
OrthoDBiEOG6BKJ7H.

Family and domain databases

Gene3Di3.30.470.20. 2 hits.
HAMAPiMF_00782. Glut_biosynth.
InterProiIPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8XK30-1 [UniParc]FASTAAdd to Basket

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MVNLDKGLLK IIKDESLEDY FIKANFGLEK ENVRVTESGN LALTPHPKAF    50
GDREKNAYIK TDFSESQLEM VTPVCNTLEE VYSFICNLNK VVSLEIMKNG 100
EFLWPQSNPP ILPREEEIPI AKLSNREDEL YRENLSYKYG KKKQVISGIH 150
YNFSFKEEFI KLLYKELKVE KDFREFKDDI YLRMARNFQK YHWLLIYLTG 200
ASPVFHESYI EEIKEEGEKL GEDSYYIKDD TSLRNSSYGY KNKKDYYVSY 250
NSIEEYASDI KNLVKDKEIQ SIKEYYNPIR LKSLGSEDML ESLLHKGIDY 300
LEVRLLDLDP LSIQGVSKET LYLLHLFMIY TLLKENKEIT YKDQEEFFKN 350
HDMVALKGRN EEAVIYENGV PVLLKDKGRE ILSEMDEIVE ILFSNNEEFK 400
NVIKRALEKI NNPHDTISEK LIKDIKEEGY INFHMRLAKE YLNNFKNKEF 450
NLVGYEDLEL STQILILDAI KRGIEFNIMD RLENFISLSD GEKVEYVKQA 500
TKTSKDSYIT ALIMENKLVT KDILRENNIR VPKGKDYDNI DEAKKDFRLF 550
KDEKIVIKPK STNFGLGISI FPGEYSREDY DKAVEIAFRE DSSILIEEFM 600
TGKEYRFLVI GEEVVGILHR EPANVIGNGE STIEELVSEK NKDPLRGKGY 650
KTPLEKIKLG EIEEMFLKNQ GLSFKSIPKN GEKIYLRENS NISTGGDSID 700
FTDKIHPSYK EVALKSAKAV KALICGVDMV IDNIEEEAKE KNHGIIELNF 750
NPAIHIHCFP YKGENRKAGE KILDLLFN 778
Length:778
Mass (Da):90,463
Last modified:March 1, 2002 - v1
Checksum:i2008FF46AF06423E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000016 Genomic DNA. Translation: BAB81279.1.
RefSeqiNP_562489.1. NC_003366.1.
WP_011010516.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB81279; BAB81279; BAB81279.
GeneIDi989883.
KEGGicpe:CPE1573.
PATRICi19497095. VBICloPer59675_1643.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000016 Genomic DNA. Translation: BAB81279.1 .
RefSeqi NP_562489.1. NC_003366.1.
WP_011010516.1. NC_003366.1.

3D structure databases

ProteinModelPortali Q8XK30.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 195102.CPE1573.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB81279 ; BAB81279 ; BAB81279 .
GeneIDi 989883.
KEGGi cpe:CPE1573.
PATRICi 19497095. VBICloPer59675_1643.

Phylogenomic databases

eggNOGi COG1181.
HOGENOMi HOG000156471.
KOi K01919.
OMAi HVEYVKN.
OrthoDBi EOG6BKJ7H.

Enzyme and pathway databases

UniPathwayi UPA00142 ; UER00209 .
UPA00142 ; UER00210 .
BioCyci CPER195102:GJFM-1619-MONOMER.

Family and domain databases

Gene3Di 3.30.470.20. 2 hits.
HAMAPi MF_00782. Glut_biosynth.
InterProi IPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR007370. Glu_cys_ligase.
IPR006335. Glut_biosynth.
[Graphical view ]
Pfami PF04262. Glu_cys_ligase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01435. glu_cys_lig_rel. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13 / Type A.

Entry informationi

Entry nameiGSHAB_CLOPE
AccessioniPrimary (citable) accession number: Q8XK30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: March 1, 2002
Last modified: September 3, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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