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Q8XK15 (GLGB2_CLOPE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB 2

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2
Alpha-(1->4)-glucan branching enzyme 2
Glycogen branching enzyme 2
Short name=BE 2
Gene names
Name:glgB2
Ordered Locus Names:CPE1588
OrganismClostridium perfringens (strain 13 / Type A) [Complete proteome] [HAMAP]
Taxonomic identifier195102 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length664 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6646641,4-alpha-glucan branching enzyme GlgB 2 HAMAP-Rule MF_00685
PRO_0000188696

Sites

Active site3421Nucleophile By similarity
Active site3951Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8XK15 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: DFFC9DBA4024081A

FASTA66477,485
        10         20         30         40         50         60 
MGGKEMRNCK ELKHEKNGNV TEKVGKNKGK SKKVSKDESL LSFDLFLEGK EHSAYKFMGA 

        70         80         90        100        110        120 
HFITENRKRG VRFTTWAPRA SKIYVIGDFN NWELKEEYSM KKINERGIWS LFLPKLEEGI 

       130        140        150        160        170        180 
KYKFAVVNEC GNNTVYKADP YAFKSELRPN TASVLTKIKS FRWGDKRWLN KREKEGLDNK 

       190        200        210        220        230        240 
PMNIYELHLG SWKRKDGEFM TYEEISEVLV EYIKEMGYTH VEFMPINEHP LDASWGYQGV 

       250        260        270        280        290        300 
GYYSVTSRYG DLNGLKTLIN KLHKNNIGVL LDWVPSHFCK DEHGLFMFDG SPTYEYEAWW 

       310        320        330        340        350        360 
KANNEGWGTC NFDLGRPEVK SFLFSNAMYW INEFHVDGLR VDAVSNMLYL DYGREYGEWE 

       370        380        390        400        410        420 
PNIYGGNGNL EAIAFLKELN TIIKKEGKGA ITVAEESTSW EGITKPVEED GLGFDYKWNM 

       430        440        450        460        470        480 
GWMNDTLSYI ELDPIYRKYH HNKMNFSMMY NYSEKFILPI SHDEVVHGKK SLINKMWGDD 

       490        500        510        520        530        540 
WKKYAGLRVY ASFMMGHPGK KLMFMGCEFG QFVEWREWEE LQWNVIEEFD IHRKTKEYFK 

       550        560        570        580        590        600 
ALNKFYLENS SLWSLDYEEE GFKWIDADNS EESVLSFIRI GKNKKEKLIF ICNFTPEVYY 

       610        620        630        640        650        660 
DFKVGVPELG EYVEAFNSDA LEFGGAGNIV GDSILKATEE SFKDFDYSIS VKVPPLGTLV 


LKVK 

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References

[1]"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater."
Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H.
Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 13 / Type A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000016 Genomic DNA. Translation: BAB81294.1.
RefSeqNP_562504.1. NC_003366.1.

3D structure databases

ProteinModelPortalQ8XK15.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING195102.CPE1588.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB81294; BAB81294; BAB81294.
GeneID989898.
KEGGcpe:CPE1588.
PATRIC19497125. VBICloPer59675_1658.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAKPTYLGG.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycCPER195102:GJFM-1634-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB2_CLOPE
AccessionPrimary (citable) accession number: Q8XK15
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2002
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries