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Protein

1,4-alpha-glucan branching enzyme GlgB 2

Gene

glgB2

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei342NucleophileBy similarity1
Active sitei395Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processCarbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164

Protein family/group databases

CAZyiCBM48 Carbohydrate-Binding Module Family 48
GH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB 2 (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2
Alpha-(1->4)-glucan branching enzyme 2
Glycogen branching enzyme 2
Short name:
BE 2
Gene namesi
Name:glgB2
Ordered Locus Names:CPE1588
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000818 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001886961 – 6641,4-alpha-glucan branching enzyme GlgB 2Add BLAST664

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8XK15
SMRiQ8XK15
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000283037
KOiK00700
OMAiHAVKNEV
OrthoDBiPOG091H08D1

Family and domain databases

Gene3Di2.60.40.10, 1 hit
2.60.40.1180, 1 hit
HAMAPiMF_00685 GlgB, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR037439 Branching_enzy
IPR006407 GlgB
IPR006047 Glyco_hydro_13_cat_dom
IPR004193 Glyco_hydro_13_N
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
IPR013783 Ig-like_fold
IPR014756 Ig_E-set
PANTHERiPTHR43651 PTHR43651, 1 hit
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PF02922 CBM_48, 1 hit
PIRSFiPIRSF000463 GlgB, 1 hit
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF81296 SSF81296, 1 hit
TIGRFAMsiTIGR01515 branching_enzym, 1 hit

Sequencei

Sequence statusi: Complete.

Q8XK15-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGKEMRNCK ELKHEKNGNV TEKVGKNKGK SKKVSKDESL LSFDLFLEGK
60 70 80 90 100
EHSAYKFMGA HFITENRKRG VRFTTWAPRA SKIYVIGDFN NWELKEEYSM
110 120 130 140 150
KKINERGIWS LFLPKLEEGI KYKFAVVNEC GNNTVYKADP YAFKSELRPN
160 170 180 190 200
TASVLTKIKS FRWGDKRWLN KREKEGLDNK PMNIYELHLG SWKRKDGEFM
210 220 230 240 250
TYEEISEVLV EYIKEMGYTH VEFMPINEHP LDASWGYQGV GYYSVTSRYG
260 270 280 290 300
DLNGLKTLIN KLHKNNIGVL LDWVPSHFCK DEHGLFMFDG SPTYEYEAWW
310 320 330 340 350
KANNEGWGTC NFDLGRPEVK SFLFSNAMYW INEFHVDGLR VDAVSNMLYL
360 370 380 390 400
DYGREYGEWE PNIYGGNGNL EAIAFLKELN TIIKKEGKGA ITVAEESTSW
410 420 430 440 450
EGITKPVEED GLGFDYKWNM GWMNDTLSYI ELDPIYRKYH HNKMNFSMMY
460 470 480 490 500
NYSEKFILPI SHDEVVHGKK SLINKMWGDD WKKYAGLRVY ASFMMGHPGK
510 520 530 540 550
KLMFMGCEFG QFVEWREWEE LQWNVIEEFD IHRKTKEYFK ALNKFYLENS
560 570 580 590 600
SLWSLDYEEE GFKWIDADNS EESVLSFIRI GKNKKEKLIF ICNFTPEVYY
610 620 630 640 650
DFKVGVPELG EYVEAFNSDA LEFGGAGNIV GDSILKATEE SFKDFDYSIS
660
VKVPPLGTLV LKVK
Length:664
Mass (Da):77,485
Last modified:March 1, 2002 - v1
Checksum:iDFFC9DBA4024081A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA Translation: BAB81294.1

Genome annotation databases

EnsemblBacteriaiBAB81294; BAB81294; BAB81294
KEGGicpe:CPE1588

Similar proteinsi

Entry informationi

Entry nameiGLGB2_CLOPE
AccessioniPrimary (citable) accession number: Q8XK15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: March 1, 2002
Last modified: February 28, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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