Q8XK15 (GLGB2_CLOPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB 2
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2
Alpha-(1->4)-glucan branching enzyme 2
Glycogen branching enzyme 2
Short name=BE 2

Gene names

Name:	glgB2
Ordered Locus Names:	CPE1588

Organism

Clostridium perfringens [Complete proteome] [HAMAP]

Taxonomic identifier

1502 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	664 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 664	664	1,4-alpha-glucan branching enzyme GlgB 2 HAMAP MF_00685		PRO_0000188696
Sites
	Active site	342	1	Nucleophile By similarity
	Active site	395	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XK15 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: DFFC9DBA4024081A	FASTA	664	77,485
	10 20 30 40 50 60 MGGKEMRNCK ELKHEKNGNV TEKVGKNKGK SKKVSKDESL LSFDLFLEGK EHSAYKFMGA 70 80 90 100 110 120 HFITENRKRG VRFTTWAPRA SKIYVIGDFN NWELKEEYSM KKINERGIWS LFLPKLEEGI 130 140 150 160 170 180 KYKFAVVNEC GNNTVYKADP YAFKSELRPN TASVLTKIKS FRWGDKRWLN KREKEGLDNK 190 200 210 220 230 240 PMNIYELHLG SWKRKDGEFM TYEEISEVLV EYIKEMGYTH VEFMPINEHP LDASWGYQGV 250 260 270 280 290 300 GYYSVTSRYG DLNGLKTLIN KLHKNNIGVL LDWVPSHFCK DEHGLFMFDG SPTYEYEAWW 310 320 330 340 350 360 KANNEGWGTC NFDLGRPEVK SFLFSNAMYW INEFHVDGLR VDAVSNMLYL DYGREYGEWE 370 380 390 400 410 420 PNIYGGNGNL EAIAFLKELN TIIKKEGKGA ITVAEESTSW EGITKPVEED GLGFDYKWNM 430 440 450 460 470 480 GWMNDTLSYI ELDPIYRKYH HNKMNFSMMY NYSEKFILPI SHDEVVHGKK SLINKMWGDD 490 500 510 520 530 540 WKKYAGLRVY ASFMMGHPGK KLMFMGCEFG QFVEWREWEE LQWNVIEEFD IHRKTKEYFK 550 560 570 580 590 600 ALNKFYLENS SLWSLDYEEE GFKWIDADNS EESVLSFIRI GKNKKEKLIF ICNFTPEVYY 610 620 630 640 650 660 DFKVGVPELG EYVEAFNSDA LEFGGAGNIV GDSILKATEE SFKDFDYSIS VKVPPLGTLV LKVK

References

[1]	"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000016 Genomic DNA. Translation: BAB81294.1.
RefSeq	NP_562504.1. NC_003366.1.
3D structure databases
ProteinModelPortal	Q8XK15.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	989898.
GenomeReviews	Gene locus CPE1588 in contig BA000016_GR.
KEGG	cpe:CPE1588.
NMPDR	fig|195102.1.peg.1651.
PATRIC	19497125. VBICloPer59675_1658.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287139.
OMA	AHFITEN.
PhylomeDB	Q8XK15.
ProtClustDB	PRK05402.
Enzyme and pathway databases
BioCyc	CPER195102:CPE1588-MONOMER.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 2 hits. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB2_CLOPE

Accession

Primary (citable) accession number: Q8XK15

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program