Q8XJE1 (DXS_CLOPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	CPE1819

Organism

Clostridium perfringens [Complete proteome] [HAMAP]

Taxonomic identifier

1502 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000189106

Sequences

	Sequence		Length	Mass (Da)
	Q8XJE1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: D4BE109383971A9C	FASTA	619	68,496
	10 20 30 40 50 60 MSEVLQRITD PKEIKDLDEK ELEILAEDLR EFLIESVSNT GGHFASNLGV IDLTVALFKN 70 80 90 100 110 120 FDFSEDRIIW DVGHQSYAYK ILTGRKDKFN TLRQYGGLCG FPKRTESEYD FFATGHSSTS 130 140 150 160 170 180 LSSAAGMARA QRLLGKDNKV IAVIGDGALT GGMALEALND IGYRKDNLII ILNDNQMSIC 190 200 210 220 230 240 KNVGGLATYL NKLRMGVGYN KLKSDIGSTL DTTSLGKRVK NSLSKLKDGI KKIVVPSMYF 250 260 270 280 290 300 EDIGLKYFGI VDGHNIRELN EVLSIAKNIK GPVIIHTVTK KGKGYELAEK NPNKYHGVSP 310 320 330 340 350 360 FDLGEGVISK FSSRNYSSTF GEEMIKLAKN DDKVVAITAA MPDGTGLKDF REEFPDRFFD 370 380 390 400 410 420 VGIAEQHAVT LAAGMAAEGL KPFFAVYSTF LQRAYDQVLH DVCIQKLPVT LCLDRAGLVG 430 440 450 460 470 480 EDGETHQGIF DISFLSPMPN MTIVAPKCID EMEVILKWAS NFNAPLAIRY PRGGDIDVNL 490 500 510 520 530 540 KPLSKIEYGK WEKVQEGEKI AIVATGKMVQ HAMIAAQKIK EEKNIDILII NATFIKPIDK 550 560 570 580 590 600 ELLNSLSKDG FKIVTIEDNI KKGGFGEGVL EYLNEIGHKE KIVTLAFNDK FIEHGKPDIL 610 YKINGLDAEG IKNTLIELL

References

[1]

"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000016 Genomic DNA. Translation: BAB81525.1.
RefSeq	NP_562735.1. NC_003366.1.
3D structure databases
ProteinModelPortal	Q8XJE1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	990128.
GenomeReviews	Gene locus CPE1819 in contig BA000016_GR.
KEGG	cpe:CPE1819.
NMPDR	fig|195102.1.peg.1882.
PATRIC	19497593. VBICloPer59675_1892.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571647.
OMA	RAHEFES.
PhylomeDB	Q8XJE1.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	CPER195102:CPE1819-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. PF00456. Transketolase_N. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_CLOPE

Accession

Primary (citable) accession number: Q8XJE1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program