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Protein

Penicillin-binding protein 1A

Gene

pbpA

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (By similarity).By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei417 – 4171Acyl-ester intermediateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciCPER195102:GJFM-2015-MONOMER.
UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Short name:
PBP1
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.2.-)
Alternative name(s):
Peptidoglycan TGase
Penicillin-sensitive transpeptidase (EC:3.4.-.-)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:pbpA
Ordered Locus Names:CPE1962
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000818 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3030CytoplasmicSequence analysisAdd
BLAST
Transmembranei31 – 5121Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini52 – 679628ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

DrugBankiDB01060. Amoxicillin.
DB01061. Azlocillin.
DB01602. Bacampicillin.
DB00833. Cefaclor.
DB00456. Cefalotin.
DB01139. Cefapirin.
DB01066. Cefditoren.
DB00267. Cefmenoxime.
DB00229. Cefotiam.
DB01112. Cefuroxime.
DB01147. Cloxacillin.
DB01000. Cyclacillin.
DB00301. Flucloxacillin.
DB00948. Mezlocillin.
DB00713. Oxacillin.
DB00417. Phenoxymethylpenicillin.
DB01604. Pivampicillin.
DB01605. Pivmecillinam.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 679679Penicillin-binding protein 1APRO_0000321877Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ8XJ01.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni72 – 244173TransglycosylaseBy similarityAdd
BLAST
Regioni378 – 663286TranspeptidaseBy similarityAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000041137.
KOiK05366.
OMAiFYFNKSV.
OrthoDBiEOG6KDKQS.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8XJ01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTERKREHKD RKQNKNSPKN QSKVTKFLKW FFIGILLLGI TAVTVVGIYV
60 70 80 90 100
LSIIRSSPEL DVQAIQSLNQ PSILYDDQGN FMDNVITREQ RYVVKSEEIP
110 120 130 140 150
DNLKKAFVAI EDERFYEHKG IDIKRIFGVI ASNIKGKLSG SNTVQGASTI
160 170 180 190 200
TQQLIKNAVL TNEVSYERKI KEMYLALELE KHLSKDEILT TYLNTIPMGG
210 220 230 240 250
YQYGVSAAAQ RFFSKNVSDL NLVECAYLGG LTQAPTSYDG LSEANKENPS
260 270 280 290 300
RYLNRTKSVL FKMHELGYIS SEQYNDAINE IDTNGIKFTP NNKLSKTNFE
310 320 330 340 350
WFTRPAITQV KQDLMDKYKY TQEEVDKLIA NGGLKIYTSM DRNLQNNVQK
360 370 380 390 400
VLDDPNNYKA ITNNPNEKNE DGVYKLQASA TIIDYKTGHV KALVGGRGEQ
410 420 430 440 450
PAMSHNRAYY DLKSIGSATK PLTVYGPAID LGLGGAGSVV NDSPLSNKEL
460 470 480 490 500
SSTGYKDQPK NEYNSYRGPL TFREAIKISS NLAAIKVANE VGVSNSIAYG
510 520 530 540 550
EKLGLVYGPH SRGISTTALG QFQNDPNNPD GGNTYTLASA FGVFGNNGVK
560 570 580 590 600
TNAKLYTKVL DSHGNVILDT STPEETKIFS PQASYIVYDM LKDQVESGSA
610 620 630 640 650
KSAKFGNIPV AGKTGTTTGD KDYLFAGLTP YYSAAIWIGY DKPREMRTSS
660 670
GTVTSPIFGK IMGLAHKDLQ YKEVDNLVE
Length:679
Mass (Da):75,177
Last modified:March 1, 2002 - v1
Checksum:iFCEE7683E6C07A3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA. Translation: BAB81668.1.
RefSeqiWP_011010706.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB81668; BAB81668; BAB81668.
GeneIDi990276.
KEGGicpe:CPE1962.
PATRICi19497889. VBICloPer59675_2035.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA. Translation: BAB81668.1.
RefSeqiWP_011010706.1. NC_003366.1.

3D structure databases

ProteinModelPortaliQ8XJ01.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

DrugBankiDB01060. Amoxicillin.
DB01061. Azlocillin.
DB01602. Bacampicillin.
DB00833. Cefaclor.
DB00456. Cefalotin.
DB01139. Cefapirin.
DB01066. Cefditoren.
DB00267. Cefmenoxime.
DB00229. Cefotiam.
DB01112. Cefuroxime.
DB01147. Cloxacillin.
DB01000. Cyclacillin.
DB00301. Flucloxacillin.
DB00948. Mezlocillin.
DB00713. Oxacillin.
DB00417. Phenoxymethylpenicillin.
DB01604. Pivampicillin.
DB01605. Pivmecillinam.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB81668; BAB81668; BAB81668.
GeneIDi990276.
KEGGicpe:CPE1962.
PATRICi19497889. VBICloPer59675_2035.

Phylogenomic databases

HOGENOMiHOG000041137.
KOiK05366.
OMAiFYFNKSV.
OrthoDBiEOG6KDKQS.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciCPER195102:GJFM-2015-MONOMER.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR001460. PCN-bd_Tpept.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13 / Type A.

Entry informationi

Entry nameiPBPA_CLOPE
AccessioniPrimary (citable) accession number: Q8XJ01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 1, 2002
Last modified: November 11, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.