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Protein

Penicillin-binding protein 1A

Gene

pbpA

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).By similarity

Catalytic activityi

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.By similarity
Preferential cleavage: (Ac)2-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei111Proton donor; for transglycosylase activityBy similarity1
Active sitei417Acyl-ester intermediate; for transpeptidase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Glycosyltransferase, Hydrolase, Multifunctional enzyme, Protease, Transferase
Biological processAntibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

UniPathwayiUPA00219

Protein family/group databases

CAZyiGT51 Glycosyltransferase Family 51

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1A
Short name:
PBP1
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.1.129By similarity)
Alternative name(s):
Peptidoglycan TGase
Penicillin-sensitive transpeptidase (EC:3.4.16.4By similarity)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:pbpA
Ordered Locus Names:CPE1962
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
Proteomesi
  • UP000000818 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30CytoplasmicSequence analysisAdd BLAST30
Transmembranei31 – 51Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini52 – 679ExtracellularSequence analysisAdd BLAST628

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

DrugBankiDB01060 Amoxicillin
DB01061 Azlocillin
DB01602 Bacampicillin
DB00833 Cefaclor
DB00456 Cefalotin
DB01139 Cefapirin
DB01066 Cefditoren
DB00267 Cefmenoxime
DB00229 Cefotiam
DB01112 Cefuroxime
DB01147 Cloxacillin
DB01000 Cyclacillin
DB04133 Degraded Cephaloridine
DB00301 Flucloxacillin
DB00447 Loracarbef
DB00948 Mezlocillin
DB00713 Oxacillin
DB00417 Phenoxymethylpenicillin
DB01604 Pivampicillin
DB01605 Pivmecillinam

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003218771 – 679Penicillin-binding protein 1AAdd BLAST679

Proteomic databases

PRIDEiQ8XJ01

Structurei

3D structure databases

ProteinModelPortaliQ8XJ01
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni72 – 244TransglycosylaseBy similarityAdd BLAST173
Regioni378 – 663TranspeptidaseBy similarityAdd BLAST286

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000041137
KOiK05366
OMAiWVGFNDS
OrthoDBiPOG091H01NC

Family and domain databases

Gene3Di1.10.3810.10, 1 hit
InterProiView protein in InterPro
IPR012338 Beta-lactam/transpept-like
IPR001264 Glyco_trans_51
IPR023346 Lysozyme-like_dom_sf
IPR036950 PBP_transglycosylase
IPR001460 PCN-bd_Tpept
PfamiView protein in Pfam
PF00912 Transgly, 1 hit
PF00905 Transpeptidase, 1 hit
SUPFAMiSSF53955 SSF53955, 1 hit
SSF56601 SSF56601, 2 hits

Sequencei

Sequence statusi: Complete.

Q8XJ01-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTERKREHKD RKQNKNSPKN QSKVTKFLKW FFIGILLLGI TAVTVVGIYV
60 70 80 90 100
LSIIRSSPEL DVQAIQSLNQ PSILYDDQGN FMDNVITREQ RYVVKSEEIP
110 120 130 140 150
DNLKKAFVAI EDERFYEHKG IDIKRIFGVI ASNIKGKLSG SNTVQGASTI
160 170 180 190 200
TQQLIKNAVL TNEVSYERKI KEMYLALELE KHLSKDEILT TYLNTIPMGG
210 220 230 240 250
YQYGVSAAAQ RFFSKNVSDL NLVECAYLGG LTQAPTSYDG LSEANKENPS
260 270 280 290 300
RYLNRTKSVL FKMHELGYIS SEQYNDAINE IDTNGIKFTP NNKLSKTNFE
310 320 330 340 350
WFTRPAITQV KQDLMDKYKY TQEEVDKLIA NGGLKIYTSM DRNLQNNVQK
360 370 380 390 400
VLDDPNNYKA ITNNPNEKNE DGVYKLQASA TIIDYKTGHV KALVGGRGEQ
410 420 430 440 450
PAMSHNRAYY DLKSIGSATK PLTVYGPAID LGLGGAGSVV NDSPLSNKEL
460 470 480 490 500
SSTGYKDQPK NEYNSYRGPL TFREAIKISS NLAAIKVANE VGVSNSIAYG
510 520 530 540 550
EKLGLVYGPH SRGISTTALG QFQNDPNNPD GGNTYTLASA FGVFGNNGVK
560 570 580 590 600
TNAKLYTKVL DSHGNVILDT STPEETKIFS PQASYIVYDM LKDQVESGSA
610 620 630 640 650
KSAKFGNIPV AGKTGTTTGD KDYLFAGLTP YYSAAIWIGY DKPREMRTSS
660 670
GTVTSPIFGK IMGLAHKDLQ YKEVDNLVE
Length:679
Mass (Da):75,177
Last modified:March 1, 2002 - v1
Checksum:iFCEE7683E6C07A3B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000016 Genomic DNA Translation: BAB81668.1

Genome annotation databases

EnsemblBacteriaiBAB81668; BAB81668; BAB81668
KEGGicpe:CPE1962

Similar proteinsi

Entry informationi

Entry nameiPBPA_CLOPE
AccessioniPrimary (citable) accession number: Q8XJ01
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 1, 2002
Last modified: May 23, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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