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Q8XJ01 (PBPA_CLOPE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Penicillin-binding protein 1A
Short name=PBP1

Including the following 2 domains:

  1. Penicillin-insensitive transglycosylase
    EC=2.4.2.-
    Alternative name(s):
    Peptidoglycan TGase
  2. Penicillin-sensitive transpeptidase
    EC=3.4.-.-
    Alternative name(s):
    DD-transpeptidase
Gene names
Name:pbpA
Ordered Locus Names:CPE1962
OrganismClostridium perfringens [Complete proteome] [HAMAP]
Taxonomic identifier1502 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length679 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Subcellular location

Cell membrane; Single-pass type II membrane protein Potential.

Sequence similarities

In the N-terminal section; belongs to the glycosyltransferase 51 family.

In the C-terminal section; belongs to the transpeptidase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 679679Penicillin-binding protein 1A
PRO_0000321877

Regions

Topological domain1 – 3030Cytoplasmic Potential
Transmembrane31 – 5121Helical; Signal-anchor for type II membrane protein; Potential
Topological domain52 – 679628Extracellular Potential
Region72 – 244173Transglycosylase By similarity
Region378 – 663286Transpeptidase By similarity

Sites

Active site4171Acyl-ester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8XJ01 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: FCEE7683E6C07A3B

FASTA67975,177
        10         20         30         40         50         60 
MTERKREHKD RKQNKNSPKN QSKVTKFLKW FFIGILLLGI TAVTVVGIYV LSIIRSSPEL 

        70         80         90        100        110        120 
DVQAIQSLNQ PSILYDDQGN FMDNVITREQ RYVVKSEEIP DNLKKAFVAI EDERFYEHKG 

       130        140        150        160        170        180 
IDIKRIFGVI ASNIKGKLSG SNTVQGASTI TQQLIKNAVL TNEVSYERKI KEMYLALELE 

       190        200        210        220        230        240 
KHLSKDEILT TYLNTIPMGG YQYGVSAAAQ RFFSKNVSDL NLVECAYLGG LTQAPTSYDG 

       250        260        270        280        290        300 
LSEANKENPS RYLNRTKSVL FKMHELGYIS SEQYNDAINE IDTNGIKFTP NNKLSKTNFE 

       310        320        330        340        350        360 
WFTRPAITQV KQDLMDKYKY TQEEVDKLIA NGGLKIYTSM DRNLQNNVQK VLDDPNNYKA 

       370        380        390        400        410        420 
ITNNPNEKNE DGVYKLQASA TIIDYKTGHV KALVGGRGEQ PAMSHNRAYY DLKSIGSATK 

       430        440        450        460        470        480 
PLTVYGPAID LGLGGAGSVV NDSPLSNKEL SSTGYKDQPK NEYNSYRGPL TFREAIKISS 

       490        500        510        520        530        540 
NLAAIKVANE VGVSNSIAYG EKLGLVYGPH SRGISTTALG QFQNDPNNPD GGNTYTLASA 

       550        560        570        580        590        600 
FGVFGNNGVK TNAKLYTKVL DSHGNVILDT STPEETKIFS PQASYIVYDM LKDQVESGSA 

       610        620        630        640        650        660 
KSAKFGNIPV AGKTGTTTGD KDYLFAGLTP YYSAAIWIGY DKPREMRTSS GTVTSPIFGK 

       670 
IMGLAHKDLQ YKEVDNLVE

« Hide

References

[1]"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater."
Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H.
Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 13 / Type A.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000016 Genomic DNA. Translation: BAB81668.1.
RefSeqNP_562878.1. NC_003366.1.

3D structure databases

ProteinModelPortalQ8XJ01.
ModBaseSearch...

Protein family/group databases

CAZyGT51. Glycosyltransferase Family 51.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID990276.
GenomeReviewsGene locus CPE1962 in contig BA000016_GR.
KEGGcpe:CPE1962.
NMPDRfig|195102.1.peg.2025.
PATRIC19497889. VBICloPer59675_2035.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG672432.
OMAMYLALEL.
ProtClustDBCLSK811156.

Enzyme and pathway databases

BioCycCPER195102:CPE1962-MONOMER.

Family and domain databases

InterProIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR011816. PBP_1a.
IPR001460. PCN-bd_Tpept.
[Graphical view]
Gene3DG3DSA:3.40.710.10. G3DSA:3.40.710.10. 1 hit.
KOK05366.
PfamPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
[Graphical view]
SUPFAMSSF56601. PBP_transp_fold. 1 hit.
TIGRFAMsTIGR02074. PBP_1a_fam. 1 hit.
ProtoNetSearch...

Other

DrugBankDB01060. Amoxicillin.
DB00415. Ampicillin.
DB01061. Azlocillin.
DB01602. Bacampicillin.
DB00578. Carbenicillin.
DB00833. Cefaclor.
DB01140. Cefadroxil.
DB00456. Cefalotin.
DB01326. Cefamandole.
DB01327. Cefazolin.
DB00535. Cefdinir.
DB01066. Cefditoren.
DB00671. Cefixime.
DB00267. Cefmenoxime.
DB00274. Cefmetazole.
DB01328. Cefonicid.
DB01329. Cefoperazone.
DB00923. Ceforanide.
DB00493. Cefotaxime.
DB00229. Cefotiam.
DB01331. Cefoxitin.
DB00430. Cefpiramide.
DB01416. Cefpodoxime.
DB01150. Cefprozil.
DB01333. Cefradine.
DB00438. Ceftazidime.
DB01415. Ceftibuten.
DB01332. Ceftizoxime.
DB01112. Cefuroxime.
DB00567. Cephalexin.
DB00689. Cephaloglycin.
DB01139. Cephapirin.
DB01147. Cloxacillin.
DB01000. Cyclacillin.
DB00485. Dicloxacillin.
DB00301. Flucloxacillin.
DB00739. Hetacillin.
DB00447. Loracarbef.
DB01603. Meticillin.
DB00948. Mezlocillin.
DB00607. Nafcillin.
DB00713. Oxacillin.
DB01053. Penicillin G.
DB00417. Penicillin V.
DB00319. Piperacillin.
DB01604. Pivampicillin.
DB01605. Pivmecillinam.

Entry information

Entry namePBPA_CLOPE
AccessionPrimary (citable) accession number: Q8XJ01
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: March 1, 2002
Last modified: January 25, 2012
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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