Reviewed, UniProtKB/Swiss-Prot Q8XIY5 (ASNA_CLOPE)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	CPE1978

Organism

Clostridium perfringens [Complete proteome] [HAMAP]

Taxonomic identifier

1502 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Aspartate--ammonia ligase HAMAP MF_00555		PRO_0000195871

Sequences

	Sequence		Length	Mass (Da)
	Q8XIY5-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: D4DA0F2DD8C30563	FASTA	336	38,954
	10 20 30 40 50 60 MEKLFIPKGY KPLLSLRETE VAIKELKDFF EDSLAKNLNL TRVSAPLFVN KGSGLNDDLN 70 80 90 100 110 120 GIERPVSFDM KAMPEFNIQI VHSLAKWKRL ALHRYEFEHG EGLYTDMNAI RRDEDLDNIH 130 140 150 160 170 180 SIYVDQWDWE KIIDKEERNL ETLKETVKSI YGTFKATEDF IVAKYPHIEK ILPEDITFIT 190 200 210 220 230 240 SQELEDRYPD LTSKERETAI CKEFGAVFII GIGGKLASGE KHDDRSPDYD DWTLNGDLLF 250 260 270 280 290 300 YYPLFDEAVE LSSMGIRVDE ESLLKQLKIA ECEERKELPF HQMLLEGKLP YTIGGGIGQS 310 320 330 RICMFFLRKA HIGEVQASMW DEDMIRTCEE NNIHLL

References

[1]

"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000016 Genomic DNA. Translation: BAB81684.1.
RefSeq	NP_562894.1.
3D structure databases
HSSP	HSSP built from PDB template 12AS based on UniProtKB P00963.
ModBase	Search...
Genome annotation databases
GeneID	990292.
GenomeReviews	Gene locus CPE1978 in contig BA000016_GR.
KEGG	cpe:CPE1978.
NMPDR	fig|195102.1.peg.2041.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8XIY5.
OMA	LNDNLNG.
Enzyme and pathway databases
BioCyc	CPER195102:CPE1978-MON.
BRENDA	6.3.1.1. 2406.
Family and domain databases
HAMAP	MF_00555. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
ProDom	PD024629. AsnA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_CLOPE

Accession

Primary (citable) accession number: Q8XIY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	March 1, 2002
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)