Q8XI68 (NTPA_CLOPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

CPE2253

Organism

Clostridium perfringens [Complete proteome] [HAMAP]

Taxonomic identifier

1502 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 204	204	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178155
Regions
	Region	8 – 13	6	Substrate binding By similarity
	Region	76 – 77	2	Substrate binding By similarity
Sites
	Metal binding	41	1	Magnesium or manganese By similarity
	Metal binding	76	1	Magnesium or manganese By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	182	1	Substrate By similarity
	Binding site	188	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XI68 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 2032381DE7349397	FASTA	204	22,847
	10 20 30 40 50 60 MKKFILASNN AHKVKEIKEI LKDFNLNILS LNEAGIDIDV EEDGKTFEEN SFKKANEIRK 70 80 90 100 110 120 YLLSKGESDF IVMADDSGLE VDYLNGAPGI YSARYAGEHG NDSKNNEKLL EELKNVKDDE 130 140 150 160 170 180 RKANFICVIV AVTDKGEKIV AEGKSYGLIL EALSGNEGFG YDPLFFVPEY KKTFAEMTSD 190 200 EKNAISHRGR ALEKLKDNLK GILK

References

[1]	"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000016 Genomic DNA. Translation: BAB81959.1.
RefSeq	NP_563169.1. NC_003366.1.
3D structure databases
ProteinModelPortal	Q8XI68.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	990606.
GenomeReviews	Gene locus CPE2253 in contig BA000016_GR.
KEGG	cpe:CPE2253.
NMPDR	fig|195102.1.peg.2316.
PATRIC	19498545. VBICloPer59675_2324.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG697237.
OMA	GVYTADW.
PhylomeDB	Q8XI68.
ProtClustDB	PRK00120.
Enzyme and pathway databases
BioCyc	CPER195102:CPE2253-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_CLOPE

Accession

Primary (citable) accession number: Q8XI68

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	March 1, 2002
Last modified:	January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program