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Q8XHE4

- SYI_CLOPE

UniProt

Q8XHE4 - SYI_CLOPE

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Protein

Isoleucine--tRNA ligase

Gene
ileS, CPE2541
Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei596 – 5961ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCPER195102:GJFM-2662-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:CPE2541
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000818: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10391039Isoleucine--tRNA ligaseUniRule annotationPRO_0000098534Add
BLAST

Proteomic databases

PRIDEiQ8XHE4.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi195102.CPE2541.

Structurei

3D structure databases

ProteinModelPortaliQ8XHE4.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionUniRule annotationAdd
BLAST
Motifi593 – 5975"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKKRIELM.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8XHE4-1 [UniParc]FASTAAdd to Basket

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MYKKVELPKG FVGVEKEVAD LWKEKNIIKK NFDMNQDGEY FTFYDGPPTA     50
NGKPHVGHVL TRVMKDIIPR YKVMKGYKVI RKAGWDTHGL PVELEIEKKL 100
GISGKPQIED YGVEKFVTEC KDSVFTYVSM WEKMTEQIGY WVDMENPYVT 150
YHNPYIESVW WALKQMWDKK LLYKGYKTMP YCPRCGTSLS SHEVSQGYKD 200
VKDLTAVAKF KVKGEDNKYF LAWTTTPWTL PSNVALCINK AYDYVEAKQA 250
EGDEIFILAK ELAPKVLGEG FEIVREFKGE ELLGMEYEQL LPFVTPTEGK 300
AFVVVHGDYV TLSDGTGIVH IAPAYGEDDN LIGKQNGLAF LNLVDLSGNF 350
VEEVTPWAGK FVKKCDQQIV DYLKEQNKLF KAEKHTHSYP HCWRCDTPLL 400
YYPKESWFVA MTTLRDKLLA NNEKINWYPD NIKNGRFGKF LENVIDWGIS 450
RDRYWGTPLP IWECECGHRE CIGSIEELKT KGINVPEDIE LHKPYIDKVH 500
LNCPHCNKEM KRTAEVIDCW FDSGSMPFAQ HHYPFENKEL FEANYPAQFI 550
SEAVDQTRGW FYTLLAISTA IFDRNPFENC IVLGHVLDKK GLKMSKSKGN 600
VVDPFEVLDS QGADATRWHF YTASAPWLPT RFSIEDVAET QRKFLSTLWN 650
VYSFYVLYAE LDNFNPLDYK DFVSDNVMDK WIMSKLNTLV KDVDDHLNNY 700
RITQAALEIE EFTDELSNWY VRRNRARYWS EELTDDKIGA YTTLYRVLVT 750
LCKVAAPFVP FITEEIYQNL VVNLDENALE SIHLGAWPEV DEKAIDKKLE 800
EKMDLAYKIV KLGRSARNGA NIKNRQPLSK MLLSTSELPE YYGDIIKDEL 850
NIKEVELGAD LSKYVNFEIK PNLPVLGRAY GKLIPAIRKE IASRNQMELA 900
QKIQNGGVEV ITVDGNEIEL NAENLLVTMQ GLEGFAFAGE GSVGVVLDTT 950
ITDELREEGH VREIISKIQN MRKESGFEVA DKITLYVAEN DMLLDVIKKF 1000
EDVIKKETLT EEIVYNGNAD YSEAKVNGEI LKMAVSKRA 1039
Length:1,039
Mass (Da):119,383
Last modified:March 1, 2002 - v1
Checksum:i9383D2B89FF0844D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000016 Genomic DNA. Translation: BAB82247.1.
RefSeqiNP_563457.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB82247; BAB82247; BAB82247.
GeneIDi990918.
KEGGicpe:CPE2541.
PATRICi19499173. VBICloPer59675_2611.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000016 Genomic DNA. Translation: BAB82247.1 .
RefSeqi NP_563457.1. NC_003366.1.

3D structure databases

ProteinModelPortali Q8XHE4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 195102.CPE2541.

Proteomic databases

PRIDEi Q8XHE4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB82247 ; BAB82247 ; BAB82247 .
GeneIDi 990918.
KEGGi cpe:CPE2541.
PATRICi 19499173. VBICloPer59675_2611.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KKRIELM.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CPER195102:GJFM-2662-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13 / Type A.

Entry informationi

Entry nameiSYI_CLOPE
AccessioniPrimary (citable) accession number: Q8XHE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2002
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi