Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8XHE4

- SYI_CLOPE

UniProt

Q8XHE4 - SYI_CLOPE

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei596 – 5961ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCPER195102:GJFM-2662-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:CPE2541
    OrganismiClostridium perfringens (strain 13 / Type A)
    Taxonomic identifieri195102 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
    ProteomesiUP000000818: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10391039Isoleucine--tRNA ligasePRO_0000098534Add
    BLAST

    Proteomic databases

    PRIDEiQ8XHE4.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi195102.CPE2541.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8XHE4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi48 – 5811"HIGH" regionAdd
    BLAST
    Motifi593 – 5975"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiKKRIELM.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8XHE4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MYKKVELPKG FVGVEKEVAD LWKEKNIIKK NFDMNQDGEY FTFYDGPPTA     50
    NGKPHVGHVL TRVMKDIIPR YKVMKGYKVI RKAGWDTHGL PVELEIEKKL 100
    GISGKPQIED YGVEKFVTEC KDSVFTYVSM WEKMTEQIGY WVDMENPYVT 150
    YHNPYIESVW WALKQMWDKK LLYKGYKTMP YCPRCGTSLS SHEVSQGYKD 200
    VKDLTAVAKF KVKGEDNKYF LAWTTTPWTL PSNVALCINK AYDYVEAKQA 250
    EGDEIFILAK ELAPKVLGEG FEIVREFKGE ELLGMEYEQL LPFVTPTEGK 300
    AFVVVHGDYV TLSDGTGIVH IAPAYGEDDN LIGKQNGLAF LNLVDLSGNF 350
    VEEVTPWAGK FVKKCDQQIV DYLKEQNKLF KAEKHTHSYP HCWRCDTPLL 400
    YYPKESWFVA MTTLRDKLLA NNEKINWYPD NIKNGRFGKF LENVIDWGIS 450
    RDRYWGTPLP IWECECGHRE CIGSIEELKT KGINVPEDIE LHKPYIDKVH 500
    LNCPHCNKEM KRTAEVIDCW FDSGSMPFAQ HHYPFENKEL FEANYPAQFI 550
    SEAVDQTRGW FYTLLAISTA IFDRNPFENC IVLGHVLDKK GLKMSKSKGN 600
    VVDPFEVLDS QGADATRWHF YTASAPWLPT RFSIEDVAET QRKFLSTLWN 650
    VYSFYVLYAE LDNFNPLDYK DFVSDNVMDK WIMSKLNTLV KDVDDHLNNY 700
    RITQAALEIE EFTDELSNWY VRRNRARYWS EELTDDKIGA YTTLYRVLVT 750
    LCKVAAPFVP FITEEIYQNL VVNLDENALE SIHLGAWPEV DEKAIDKKLE 800
    EKMDLAYKIV KLGRSARNGA NIKNRQPLSK MLLSTSELPE YYGDIIKDEL 850
    NIKEVELGAD LSKYVNFEIK PNLPVLGRAY GKLIPAIRKE IASRNQMELA 900
    QKIQNGGVEV ITVDGNEIEL NAENLLVTMQ GLEGFAFAGE GSVGVVLDTT 950
    ITDELREEGH VREIISKIQN MRKESGFEVA DKITLYVAEN DMLLDVIKKF 1000
    EDVIKKETLT EEIVYNGNAD YSEAKVNGEI LKMAVSKRA 1039
    Length:1,039
    Mass (Da):119,383
    Last modified:March 1, 2002 - v1
    Checksum:i9383D2B89FF0844D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000016 Genomic DNA. Translation: BAB82247.1.
    RefSeqiNP_563457.1. NC_003366.1.

    Genome annotation databases

    EnsemblBacteriaiBAB82247; BAB82247; BAB82247.
    GeneIDi990918.
    KEGGicpe:CPE2541.
    PATRICi19499173. VBICloPer59675_2611.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000016 Genomic DNA. Translation: BAB82247.1 .
    RefSeqi NP_563457.1. NC_003366.1.

    3D structure databases

    ProteinModelPortali Q8XHE4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 195102.CPE2541.

    Proteomic databases

    PRIDEi Q8XHE4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB82247 ; BAB82247 ; BAB82247 .
    GeneIDi 990918.
    KEGGi cpe:CPE2541.
    PATRICi 19499173. VBICloPer59675_2611.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi KKRIELM.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CPER195102:GJFM-2662-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 2 hits.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 13 / Type A.

    Entry informationi

    Entry nameiSYI_CLOPE
    AccessioniPrimary (citable) accession number: Q8XHE4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3