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Q8XHE4

- SYI_CLOPE

UniProt

Q8XHE4 - SYI_CLOPE

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Clostridium perfringens (strain 13 / Type A)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei596 – 5961ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCPER195102:GJFM-2662-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CPE2541
OrganismiClostridium perfringens (strain 13 / Type A)
Taxonomic identifieri195102 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium
ProteomesiUP000000818: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10391039Isoleucine--tRNA ligasePRO_0000098534Add
BLAST

Proteomic databases

PRIDEiQ8XHE4.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi195102.CPE2541.

Structurei

3D structure databases

ProteinModelPortaliQ8XHE4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi593 – 5975"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKKRIELM.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8XHE4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYKKVELPKG FVGVEKEVAD LWKEKNIIKK NFDMNQDGEY FTFYDGPPTA
60 70 80 90 100
NGKPHVGHVL TRVMKDIIPR YKVMKGYKVI RKAGWDTHGL PVELEIEKKL
110 120 130 140 150
GISGKPQIED YGVEKFVTEC KDSVFTYVSM WEKMTEQIGY WVDMENPYVT
160 170 180 190 200
YHNPYIESVW WALKQMWDKK LLYKGYKTMP YCPRCGTSLS SHEVSQGYKD
210 220 230 240 250
VKDLTAVAKF KVKGEDNKYF LAWTTTPWTL PSNVALCINK AYDYVEAKQA
260 270 280 290 300
EGDEIFILAK ELAPKVLGEG FEIVREFKGE ELLGMEYEQL LPFVTPTEGK
310 320 330 340 350
AFVVVHGDYV TLSDGTGIVH IAPAYGEDDN LIGKQNGLAF LNLVDLSGNF
360 370 380 390 400
VEEVTPWAGK FVKKCDQQIV DYLKEQNKLF KAEKHTHSYP HCWRCDTPLL
410 420 430 440 450
YYPKESWFVA MTTLRDKLLA NNEKINWYPD NIKNGRFGKF LENVIDWGIS
460 470 480 490 500
RDRYWGTPLP IWECECGHRE CIGSIEELKT KGINVPEDIE LHKPYIDKVH
510 520 530 540 550
LNCPHCNKEM KRTAEVIDCW FDSGSMPFAQ HHYPFENKEL FEANYPAQFI
560 570 580 590 600
SEAVDQTRGW FYTLLAISTA IFDRNPFENC IVLGHVLDKK GLKMSKSKGN
610 620 630 640 650
VVDPFEVLDS QGADATRWHF YTASAPWLPT RFSIEDVAET QRKFLSTLWN
660 670 680 690 700
VYSFYVLYAE LDNFNPLDYK DFVSDNVMDK WIMSKLNTLV KDVDDHLNNY
710 720 730 740 750
RITQAALEIE EFTDELSNWY VRRNRARYWS EELTDDKIGA YTTLYRVLVT
760 770 780 790 800
LCKVAAPFVP FITEEIYQNL VVNLDENALE SIHLGAWPEV DEKAIDKKLE
810 820 830 840 850
EKMDLAYKIV KLGRSARNGA NIKNRQPLSK MLLSTSELPE YYGDIIKDEL
860 870 880 890 900
NIKEVELGAD LSKYVNFEIK PNLPVLGRAY GKLIPAIRKE IASRNQMELA
910 920 930 940 950
QKIQNGGVEV ITVDGNEIEL NAENLLVTMQ GLEGFAFAGE GSVGVVLDTT
960 970 980 990 1000
ITDELREEGH VREIISKIQN MRKESGFEVA DKITLYVAEN DMLLDVIKKF
1010 1020 1030
EDVIKKETLT EEIVYNGNAD YSEAKVNGEI LKMAVSKRA
Length:1,039
Mass (Da):119,383
Last modified:March 1, 2002 - v1
Checksum:i9383D2B89FF0844D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000016 Genomic DNA. Translation: BAB82247.1.
RefSeqiNP_563457.1. NC_003366.1.
WP_011010955.1. NC_003366.1.

Genome annotation databases

EnsemblBacteriaiBAB82247; BAB82247; BAB82247.
GeneIDi990918.
KEGGicpe:CPE2541.
PATRICi19499173. VBICloPer59675_2611.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000016 Genomic DNA. Translation: BAB82247.1 .
RefSeqi NP_563457.1. NC_003366.1.
WP_011010955.1. NC_003366.1.

3D structure databases

ProteinModelPortali Q8XHE4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 195102.CPE2541.

Proteomic databases

PRIDEi Q8XHE4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB82247 ; BAB82247 ; BAB82247 .
GeneIDi 990918.
KEGGi cpe:CPE2541.
PATRICi 19499173. VBICloPer59675_2611.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KKRIELM.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CPER195102:GJFM-2662-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 2 hits.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13 / Type A.

Entry informationi

Entry nameiSYI_CLOPE
AccessioniPrimary (citable) accession number: Q8XHE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3