Reviewed, UniProtKB/Swiss-Prot Q8XHB2 (CARA_CLOPE)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	CPE2573

Organism

Clostridium perfringens [Complete proteome] [HAMAP]

Taxonomic identifier

1502 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112268
Regions
	Domain	170 – 349	180	Glutamine amidotransferase type-1
	Region	1 – 166	166	CPSase HAMAP MF_01209
Sites
	Active site	245	1	Nucleophile By similarity
	Active site	327	1	By similarity
	Active site	329	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8XHB2-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 1DDC6BBD9FD2045B	FASTA	349	39,083
	10 20 30 40 50 60 MKAKLILENG VVFEGKAFGY LKECVGEVVF NTGMTGYQEV LTDPSYYGQI VTMTYPLIGN 70 80 90 100 110 120 YGINLEDLES KEPKVRGFIV REKCQYPNNF RCELELETYL AQNKVLGLDG IDTRALTKIL 130 140 150 160 170 180 RNNGTMKGII VLDNSNLEDV KDKLEAFSNR DAVSIVSTNE KYEISGEGKK VAIIDFGIKQ 190 200 210 220 230 240 NIIRNFVKRG CNVTVFPYDF KAEEVLEINP DLVFLSNGPG DPEDMGEAVN EIKKIVGKKP 250 260 270 280 290 300 IVGICLGHQL LALTLGGETK KLKFGHRGCN HPVKDLINNR VHITSQNHGY YVATLPENME 310 320 330 340 ITHVSMNDGT VEGMKHKELP IFSVQFHPEA CPGPKDSEYI FDEFMKYAL

References

[1]

"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater." Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H. Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 13 / Type A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000016 Genomic DNA. Translation: BAB82279.1.
RefSeq	NP_563489.1.
3D structure databases
HSSP	HSSP built from PDB template 1M6V based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	990950.
GenomeReviews	Gene locus CPE2573 in contig BA000016_GR.
KEGG	cpe:CPE2573.
NMPDR	fig|195102.1.peg.2636.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8XHB2.
OMA	Q8XHB2. LFDGSNC.
Enzyme and pathway databases
BioCyc	CPER195102:CPE2573-MON.
BRENDA	6.3.5.5. 2406.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_CLOPE

Accession

Primary (citable) accession number: Q8XHB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	March 1, 2002
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)