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Reviewed, UniProtKB/Swiss-Prot Q8XHB2 (CARA_CLOPE)

Last modified June 16, 2009. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain
Gene names
Name: carA
Ordered Locus Names: CPE2573
OrganismClostridium perfringens [Complete proteome] [HAMAP]
Taxonomic identifier1502 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Catalytic activity

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209

Subunit structure

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.

Sequence similarities

Belongs to the carA family.

Contains 1 glutamine amidotransferase type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349Carbamoyl-phosphate synthase small chain HAMAP MF_01209
PRO_0000112268

Regions

Domain170 – 349180Glutamine amidotransferase type-1
Region1 – 166166CPSase HAMAP MF_01209

Sites

Active site2451Nucleophile By similarity
Active site3271 By similarity
Active site3291 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8XHB2-1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 1DDC6BBD9FD2045B

FASTA34939,083
        10         20         30         40         50         60 
MKAKLILENG VVFEGKAFGY LKECVGEVVF NTGMTGYQEV LTDPSYYGQI VTMTYPLIGN 

        70         80         90        100        110        120 
YGINLEDLES KEPKVRGFIV REKCQYPNNF RCELELETYL AQNKVLGLDG IDTRALTKIL 

       130        140        150        160        170        180 
RNNGTMKGII VLDNSNLEDV KDKLEAFSNR DAVSIVSTNE KYEISGEGKK VAIIDFGIKQ 

       190        200        210        220        230        240 
NIIRNFVKRG CNVTVFPYDF KAEEVLEINP DLVFLSNGPG DPEDMGEAVN EIKKIVGKKP 

       250        260        270        280        290        300 
IVGICLGHQL LALTLGGETK KLKFGHRGCN HPVKDLINNR VHITSQNHGY YVATLPENME 

       310        320        330        340 
ITHVSMNDGT VEGMKHKELP IFSVQFHPEA CPGPKDSEYI FDEFMKYAL 

« Hide

References

[1]"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater."
Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H.
Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 13 / Type A.

Cross-references

Sequence databases

BA000016 Genomic DNA. Translation: BAB82279.1.
RefSeqNP_563489.1.

3D structure databases

HSSPHSSP built from PDB template 1M6V based on UniProtKB P00907.
ModBaseSearch...

Genome annotation databases

GeneID990950.
GenomeReviewsGene locus CPE2573 in contig BA000016_GR.
KEGGcpe:CPE2573.
NMPDRfig|195102.1.peg.2636.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ8XHB2.
OMAQ8XHB2. LFDGSNC.

Enzyme and pathway databases

BioCycCPER195102:CPE2573-MON.
BRENDA6.3.5.5. 2406.

Family and domain databases

HAMAPMF_01209.
[Tree]
InterProIPR006220. Anth_synthII.
IPR001317. CarbamoylP_synth_GATase.
IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR011702. GATASE.
IPR017926. GATASE_1.
IPR000991. GATase_class1_C.
[Graphical view]
PANTHERPTHR11405:SF4. CarA_synth_small. 1 hit.
PfamPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
PRINTSPR00097. ANTSNTHASEII.
PR00099. CPSGATASE.
PR00096. GATASE.
TIGRFAMsTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCARA_CLOPE
AccessionPrimary (citable) accession number: Q8XHB2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: March 1, 2002
Last modified: June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents